A5111883256- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Biosimilars and Bioanalytical Methods
- Pregnancy and Medication Impact
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- ATP Synthase and ATPases Research
- Hematopoietic Stem Cell Transplantation
- Preterm Birth and Chorioamnionitis
- Acute Myeloid Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Neonatal Respiratory Health Research
- Macrophage Migration Inhibitory Factor
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neutropenia and Cancer Infections
- Sepsis Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Immune responses and vaccinations
- Acute Lymphoblastic Leukemia research
- Neonatal and Maternal Infections
- Renal function and acid-base balance
- Nanofabrication and Lithography Techniques
- Advancements in Semiconductor Devices and Circuit Design
Memorial Sloan Kettering Cancer Center
2021-2024
Rosalind Franklin University of Medicine and Science
2004-2015
American Society for Reproductive Medicine
2015
American Society for Reproductive Immunology
2015
Translational Research Institute
2015
Foundation for Blood Research
2012
Charité - Universitätsmedizin Berlin
2008
Circulating microRNAs (miRNAs) have emerged as candidate biomarkers of various diseases and conditions including malignancy pregnancy. This approach requires sensitive accurate quantitation miRNA concentrations in body fluids. Herein we report that enzyme-based quantitation, which is currently the mainstream for identifying differences abundance among samples, skewed by endogenous serum factors co-purify with miRNAs anticoagulant agents used during collection. Of importance, different were...
The aim of this study was to investigate the functional status and immunophenotypic characteristics natural killer (NK) cells in women who suffer recurrent spontaneous abortions (RSA) or have infertility unknown aetiology. Peripheral blood mononuclear (PBMC) were obtained from 40 patients 13 normal healthy multiparous controls. NK identified using anti-CD56 anti-CD16 monoclonal antibodies (mAb). expression CD69, CD25, CD122, CD30, CD154, CD128 CD94 on detected specific mAb analysed by flow...
Decidual natural killer (NK) cells express inhibitory receptors (killer immunoglobulin-like receptors, KIRs), which bind to ligands on trophoblast (human leucocyte antigen, HLA-C). This interaction appears block NK cytotoxicity against cells. In this study, we investigated the expression of and activating in peripheral blood women with recurrent spontaneous abortion (RSA) or implantation failures.CD56(dim)/CD16(+), CD56(bright)/CD16(-) CD56(+)/CD3(+) NKT RSA vitro fertilization (IVF)...
<p>Supplementary Figure 3. Kaplan-Meier survival curves for OS and PFS. Comparisons include (A) (B) PFS CD4 <155 !155 CD4+ T cell counts above below the median day 30 metric of 155 cells/μL. P-values are derived from multivariate Cox proportional hazards model stratified by CAR-T product adjusted confounders, including pre-CAR-T age, LDH pre-lymphodepletion, bridging.</p>
<p>Supplementary Figure 5. Gating strategy for the TBNK assay. (A) FSC vs SSC dot plot was used to identify debris, characterized as low and FSC. Non-debris events are subsequent gating. (B) Using a FSC-A FSC-H plot, singlet gate isused include remove doublet events. (C) Singlet in CD45 is set around CD45+ lymphocyte events, by bright CD45. (D) arethen passed through CD3 CD19 CD19+ B cells CD3+ T cells. (E) further identified CD3+CD4+ helper CD3+CD8+ cytotoxic CD4 CD8 plot. (F)...
<div>Abstract<p>Patients treated with chimeric antigen receptor T-cell (CAR-T) therapy are subject to profound immunosuppression. Dynamics of immune reconstitution (IR) and impacts IR on outcomes following infusion across CAR-T products not well understood. In this study, we profiled in 263 patients relapsed/refractory large B-cell lymphoma receiving (axicabtagene ciloleucel 44.9%, lisocabtagene maraleucel 30.4%, tisagenlecleucel 24.7%). Following infusion, remain persistently...
<p>Supplementary Figure 2. Boxplots with superimposed dot plots demonstrate immune subset distributions at clinically significant timepoints, including days 30, 100, 180, and 365 post-CAR-T. Axi-cel in highlighted blue, liso-cel green, tisa-cel red. Subsets shown are (A) CD4+CCR7-45RA+ effector cells, (B) CD4+CCR7+45RA+ naïve (C) CD4+CCR7+45RA- CM (D) CD8+CCR7+45RA+ (E) CD8+CCR7-45RA+ TEMRA (F) CD8+CCR7-45RA- EM (G) CD8+CCR7+45RA- cells. Ranges of 20 to 45, 80 120, 150 210, 300 400...
<p>Supplementary Figure 1. Immune subset trajectories from time of CAR-T (time 0) through one year 365) following with superimposed boxplots at predefined time-points. Subsets include (A) CD3+ T cells, (B) CD3-19+ B (C) CD3-56+16+ NK (D) CD4+ (E) CD4+CCR7-45RA- EM (F) CD4+CCR7-45RA+ effector (G) CD4+CCR7+45RA- CM (H) CD4+CCR7+45RA+ naïve (I) CD8+ (J) CD8+CCR7-45RA- (K) CD8+CCR7-45RA+ TEMRA (L) CD8+CCR7+45RA- and (M) CD8+CCR7+45RA+ cells. Trajectories depicted are estimated using LOESS...
<p>Supplementary Figure 7. Gating strategy for the T regulatory cells assay. (A, B) CD3+CD4+ helper are passed through a CD25 vs CD127 dot plot to identify CD4+CD127-CD25+ cells, and (C) CCR4 CCR4+CD25+ cells. defined as CD4+CD127-CCR4+CD25+ via series of AND gates.</p>
<p>Supplementary Figure 4. Hazard ratios for OS (in blue) and PFS red) derived from (A) unadjusted Cox proportional hazards models, (B) models stratified by CAR-T product, (C) both product adjusted confounders, including pre-CAR-T age, LDH pre- lymphodepletion, bridging. Each landmark time on the X-axis represents at which are landmarked. For instance, hazard corresponding to 35 utilizes day 20 metrics (using latest measurement within pre-specified range of days 10–35). Similarly, 75...
<p>Supplementary Figure 6. Gating strategy for the T cell subsets assay. (A, B) CD3+CD4+ helper cells are passed through a CCR7 vs CD45RA dot plot to identify CD4+CCR7+CD45RA+ naïve cells, CD4+CCR7+CD45RA- central memory (CM) CD4+CCR7-CD45RA- effector (EM) and CD4+CCR7-CD45RA+ cells. (C, D) Similarly, CD3+CD8+ cytotoxic CD8+CCR7+CD45RA+ CD8+CCR7+CD45RA- CM CD8+CCR7-CD45RA- EM CD8+CCR7-CD45RA+ (TEMRA) cells.</p>
Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell and cytokine production. We investigated whether women with recurrent pregnancy losses (RPLs) implantation failures have aberrant correlation between NCRs intracellular expression of cells.Peripheral blood cells (CD56(dim) CD56(bright)) were analyzed for (NKp46, NKp44 NKp30) (TNF-alpha, IFN-gamma, IL-4, IL-10) using flow cytometry in RPL (n = 22), 23) or controls 15).In type 1 studies, CD56(bright)/NKp30(+) (r...
We aim to determine whether peripheral blood T cell activation is associated with repeated implantation failures or recurrent pregnancy losses (RPLs).Women a history of failure (n = 18) RPLs 17) comprise the study group. Normal fertile women 11) are included as controls. Proportion activated cells (CD69(+), CD154(+)) and Th1/Th2 ratios measured by flow cytometric analysis.Proportions (%) CD4(+)/154(+) CD4(+) CD8(+)/154(+) CD8(+) were significantly higher in group than those Proportions...
Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell and cytokine production. a2V‐ATPase is expressed on subsets of PBMC regulates the extracellular environment, facilitates or secretion. In this study, we aim to investigate expression NCRs in peripheral blood cells women with recurrent spontaneous abortions (RSA) implantation failures. Method study Peripheral (CD56 dim CD56 bright were analyzed for (NKp46, NKp44 NKp30) using 3‐color flow cytometry RSA...
a2V-ATPase is the a2 isoform of vacuolar ATPase and expressed in human trophoblast cells. resides as a 70-kDa molecule intracellular vesicles. Upon cell stimulation, it migrates to surface 50-kDa molecule, after 20-kDa portion [N-terminus domain (a2NTD)] cleaved secreted extracellular environment. Previous studies showed that a2NTD-regulated cytokine production from stimulated T The aim this study was determine if a2NTD can regulate immune cells were contact with JEG-3 cells.Peripheral blood...
Abstract Neutrophils kill microorganisms by inducing exocytosis of granules with antibacterial properties. Four isoforms the “a” subunit V-ATPase—a1V, a2V, a3V, and a4V—have been identified. a2V is expressed in white blood cells, that is, on surface monocytes or activated lymphocytes. Neutrophil associated-a2V was found membranes primary (azurophilic) less often secondary (specific) granules, tertiary (gelatinase granules), secretory vesicles. However, it not resting neutrophils. Following...
Problem: Regeneration and tolerance factor (RTF) has been recently suggested to contribute the control of fetal‐ablating immunity at maternal–fetal interface through induction T helper 2 (Th2)‐dominated response. The protein consists a membrane‐associated domain an extracellular portion which is proteolitically cleaved yield soluble peptide. In humans, it shown be expressed by invading cytotrophoblasts decidual lymphoid cells, increased on peripheral blood B lymphocytes during normal...