Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques that allow for accurate diagnoses. Currently, the fastest volumetric technique is Fourier-domain full-field optical coherence tomography (FD-FF-OCT), which uses a camera rapidly tunable laser source. Here, we demonstrate high-resolution, high-speed, non-contact corneal vivo with FD-FF-OCT can acquire single 3D volume voxel rate of 7.8 GHz. The spatial source was suppressed to prevent it from...

Despite the rapid development of optical imaging methods, high-resolution in vivo with penetration into deeper tissue layers is still a major challenge. Optical coherence tomography (OCT) has been used successfully for non-invasive human retinal volumetric vivo, advancing detection, diagnosis, and monitoring various diseases. However, there are important limitations OCT imaging, especially coherent noise limited axial range over which high resolution images can be acquired. The prevents...

Droplet microfluidics disrupted analytical biology with the introduction of digital polymerase chain reaction and single-cell sequencing. The same technology may also bring important innovation in analysis bacteria, including antibiotic susceptibility testing at level. Still, despite promising demonstrations, lack a high-throughput label-free method detecting bacteria nanoliter droplets prohibits most interesting strains widespread use droplet technologies microbiology. We sensitive fast...

In this Letter, we introduce an algorithm that overcomes limitations in shape measurement by holographic microscopic methods cases of micro-optical elements with high NA, such as microlenses. The presented provides a simple method for reconstruction from interferometrically measured phase. is based on the analysis local ray transition through object. We develop algorithms configurations working transmission and reflection. accuracy developed proved experiments extensive simulations. present...

High-precision topography measurement of micro-objects using interferometric and holographic techniques can be realized provided that the in-focus plane an imaging system is very accurately determined. Therefore, in this paper we propose accurate technique for determination, which based on coherent incoherent light. The proposed method consists two major steps. First, a calibration with amplitude object performed common autofocusing illumination, allows localization position. In second step,...

In this paper a new high NA shape measurement technique working with an arbitrary spherical wave illumination is presented. The main contribution of work are formulas, derived from exact reflection and refraction laws for both the transmission configurations, which enable accurate calculations in systems location illuminating point source. proposed algorithms permit multiple samples shapes using single hologram. An accuracy method confirmed numerical simulations, show superiority approach...

In this Letter, a novel concept based on superresolution technique that enables the measurement of high gradient and deep topography objects using digital holographic (DH) microscopy is introduced. The major problem DH systems limited NA prohibits metrological characterization object features frequencies. proposed has ability to extend spatial frequency spectrum measured by applying multidirectional plane wave illumination, which experimentally realized with grating. recovers sample from set...

Spatio-Temporal Optical Coherence Tomography (STOC-T) is a novel imaging technique using light with controlled spatial and temporal coherence. Retinal images obtained the STOC-T system maintain high resolution in all three dimensions, on sample of about 700 μm, without need for mechanical scanning. In present work, we use known data processing algorithms optical coherence tomography angiography (OCTA) modify them to improve rendering vasculature human retina at different depths by...

Spatiotemporal optical coherence tomography (STOC-T) is the novel modality for high-speed, crosstalk- and aberration-free volumetric imaging of biological tissue in vivo. STOC-T extends Fourier-Domain holographic Optical Coherence Tomography by spatial phase modulation that enables reduction tunable laser. By reducing laser, we suppress coherent noise, and, consequently, improve depth. Furthermore, remove geometrical aberrations computationally postprocessing. We recently demonstrated...

In this paper we present a method for topography measurement of high gradient reflective microstructures that overcomes the limited numerical aperture (NA) digital holographic (DH) system working in reflection. We consider case when DH is unable to register light reflected from full sample area due insufficient NA. To overcome problem, propose holography microscope configuration with an afocal imaging and modified object arm setup. The proposed modification includes application spatial...

In this paper the concept, design and realization of an integrated laser interferometer are presented. The Twyman–Green-type is based on micro-optical diffraction gratings in resonance domain. fabrication process these their assembly functional will be discussed detail. interferometer, available array configuration 5 × channels, part a test set-up for fast characterization M(O)EMS devices at wafer level. first results interferometric measurements MEMS object

The degeneration of Fraunhofer diffraction conditions in the optical system with converging spherical wave illumination for bacteria species identification based on patterns is analyzed by digital holographic methods. obtained results have shown that colonies act as biological lenses time-dependent light focusing properties, which are characterized and monitored means phase retrieval from sequentially captured holograms. This significantly affects location observation plane, continuously...

The paper is devoted to characterization of topography micro optical elements with very high numerical aperture using digital holographic microscope. For we mean the one larger than system conjugating object plane detector plane. In this case not capable capturing any information about element areas (high shape gradients). are presenting method that can be used for recovering from few measurements microscope working in transmission and reflective configuration. We focusing on metrology...

The paper presents modifications of full-field optical methods commonly used to test the surface quality components for forming a high power laser beam and tests final wavefront. in reference measurements rely on implementation novel fringe pattern processing including improvement initial interferogram analysis reconstructed phase based Hilbert-Huang transform aided by principal component analysis. Also Point Diffraction Interferometer as efficient tool elements with NA is introduced. In...

Despite the rapid development of OCT, high-resolution in vivo imaging human eye with penetration into deeper retinal layers and choroid is still a major challenge due to its sensitivity coherent noise, such as speckle crosstalk. To address that, we have developed technique termed Spatio-Temporal Optical Coherence Tomography (STOC-T) that uses light controlled spatial temporal coherence obtain high-contrasted coronal projection images at various depths including choriocapillaris. It can also...

Almost hidden residual defects of a test surface can be revealed using high precision instrument such as point diffraction interferometer (PDI). In general, PDI is engaged to display the figure or wavefront with subnanometer accuracy paying attention low-frequency configurations. Such technique suited EUV X-ray optics. The tool described in paper able map absolute profile deviations several angstroms and therefore it provides new vision under various quality, e.g. detects specific...

This work focuses on the topography measurement of high NA microlenses using digital holography (DH) in microscopic setup. The necessary modifications are discussed to provide DH with capability accurate characterization microlens NA.

This paper presents the study on accuracy of topography measurement high numerical aperture focusing microobjects in digital holographic microscope setup. The system works reflective configuration with spherical wave illumination. For reconstruction NA we are using two algorithms: Thin Element Approximation (TEA) and Spherical Local Ray (SLRA). In this show comparison results these algorithms. We superiority SLRA method. However, to obtain accurate experimental conditions have be determined:...

The most suited techniques for quantitative and accurate determination of the phase distribution in a photonic microstructures are based on interferometry, especially digital holography (DH) microscopic configuration. However there is well known limitation coherent full- field interferometric measurements: difference between neighboring samples cannot be larger than 2π, or objects shape have to generate light that can collected by used optical system. This might overcame use well-known...

Optical coherence tomography (OCT) is a well-established modality providing cross-sectional images of the human retina. OCT, however, does not provide high-resolution en face outer retinal layers due to eye aberrations and tradeoff between imaging depth transverse resolution. Recently, we demonstrated spatiotemporal optical (STOC) that provides high-resolution, aberration-free volumetric retina cornea with voxel rate 8 GHz. Here, show STOC can be used for angiography, allows us estimate...

Fourier-domain full-field optical coherence tomography (FD-FF-OCT) is currently the fastest volumetric imaging technique that able to generate a single 3D data volume in less than 10 ms. FD-FF-OCT based on fast camera, rapidly tunable laser source and signal detection. However, crosstalk corrupts images with speckle therefore lowers image quality. Corneal also challenging since focuses down retina causing safety concerns. We present spatially incoherent system reduces noise enables corneal...