A5066529154- Photosynthetic Processes and Mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- Metal complexes synthesis and properties
- Hemoglobin structure and function
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Crystallization and Solubility Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- X-ray Diffraction in Crystallography
- Magnetism in coordination complexes
- Biochemical Analysis and Sensing Techniques
- Electrochemical Analysis and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Lipid Membrane Structure and Behavior
- Oxidative Organic Chemistry Reactions
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- melanin and skin pigmentation
- Crystallography and molecular interactions
- RNA regulation and disease
- Mass Spectrometry Techniques and Applications
- Radiation Therapy and Dosimetry
- Material Dynamics and Properties
- Porphyrin and Phthalocyanine Chemistry
- Advanced Electron Microscopy Techniques and Applications
Saitama University
2022-2024
RIKEN
2018-2019
University of Hyogo
2009-2018
Royal Society of Chemistry
2015
Hyogo University
2011-2012
Kyushu Lutheran College
2007
A series of Ru(IV)-oxo complexes (4–6) were synthesized from the corresponding Ru(II)-aqua (1–3) and fully characterized by 1H NMR resonance Raman spectroscopies, ESI-MS spectrometry. Based on diamagnetic character confirmed spectroscopy in D2O, spin states 5 6 determined to be S = 0 d4 configuration, sharp contrast that 4 being 1 state. The aqua-complexes 1–3 catalyzed oxidation alcohols olefins using (NH4)2[CeIV(NO3)6] (CAN) as an electron-transfer oxidant acidic aqueous solutions....
Abstract Square‐planar nickel(II) complexes of salen ligands, N , ′‐bis(3‐ tert ‐butyl‐(5 R )‐salicylidene)‐1,2‐cyclohexanediamine), in which R= ‐butyl ( 1 ), OMe 2 and NMe 3 were prepared the electronic structure one‐electron‐oxidized species [ – ] +. was investigated solution. Cyclic voltammograms showed two quasi‐reversible redox waves that assigned to oxidation phenolate moieties phenoxyl radicals. From difference between first second potentials, trend delocalization > obtained. The...
Bovine cytochrome c oxidase (CcO), a 420-kDa membrane protein, pumps protons using electrostatic repulsion between transferred through water channel and net positive charges created by oxidation of heme (Fe ) for reduction O2 at a3 a3). For this process to function properly, timing is essential: The must be closed after collection the pumped before Fe oxidation. If were remain open, spontaneous backflow collected would occur. elucidation closure mechanism, opening channel, which occurs upon...
Tyrosinase (EC 1.14.18.1), a copper-containing monooxygenase, catalyzes the conversion of phenol to corresponding ortho-quinone. The Streptomyces tyrosinase is generated as complex with “caddie” protein that facilitates transport two copper ions into active center. In our previous study, Tyr98 residue in caddie protein, which accommodated pocket center tyrosinase, has been found be converted reactive quinone through formations μ-η2:η2-peroxo-dicopper(II) and Cu(II)-dopasemiquinone...
Multiparameter single-molecule fluorescence measurement is a promising technique for detecting and quantifying heterogeneity in freely diffusing molecules, albeit with challenges data analysis due to limited photon numbers. Here, we present universal analytical framework that leverages independent component unmixing multiparameter signals. Applications static dynamic mixture systems demonstrate its potential allowing model-free separation of subpopulations microsecond time resolution...
Mechanistic insights were gained into hydrogen-atom transfer reactions from benzyl alcohol derivatives with different oxidation potentials to a highly reactive Cr(<sc>v</sc>)–oxo complex reveal switching of reaction mechanisms.
Ruthenium(II)-acetonitrile complexes having η(3)-tris(2-pyridylmethyl)amine (TPA) with an uncoordinated pyridine ring and diimine such as 2,2'-bipyridine (bpy) 2,2'-bipyrimidine (bpm), [Ru(II)(η(3)-TPA)(diimine)(CH(3)CN)](2+), reacted m-chloroperbenzoic acid to afford corresponding Ru(II)-acetonitrile pyridine-N-oxide arm, [Ru(II)(η(3)-TPA-O)(diimine)(CH(3)CN)](2+), retention of the coordination environment. Photoirradiation acetonitrile η(3)-TPA arm afforded a mixture...
本研究は、就学前の知的障害児通園施設の在籍児童の母親230名を対象に、就学前の自閉症児をもつ母親のストレッサーについて明らかにした。230名の母親は、自閉症児をもつ母親(自閉群)115名とそれ以外の母親(非自閉群)115名に分けられ、ストレッサーの相違が明らかにされた。質問紙法による調査の結果は次のようになった。(1)就学前の自閉症児をもつ母親のストレッサーには、「問題行動」「サポート不足」「愛着困難」「否定的感情」という4つの因子が見いだされた。(2)「問題行動」「愛着困難」因子に関しては、自閉群のほうが非自閉群よりも因子得点が有意に高かった。「サポート不足」「否定的感情」因子は両群に有意差はみられなかった。(3)ストレス反応の得点は、自閉群のほうが非自閉群に比べて有意に高かった。本研究によって、愛着の視点も含まれた就学前の自閉症児をもつ母親のストレッサー尺度が新たに作成された。
The geometric and electronic structure of a bimetallic Cu Schiff-base complex its one-electron oxidized form have been investigated. two salen units in the neutral 1 are linked via bridging catecholate function, coupling between Cu(II) d(9) centres was determined to be weakly antiferromagnetic on basis solid-state magnetic studies (J = -3 cm(-1)), variable-temperature electron paramagnetic resonance (EPR) cm(-1)). Theoretical calculations (DFT) were agreement with experimental results -7...
Tyrosinase (EC 1.14.18.1), which possesses two copper ions at the active center, catalyzes a rate-limiting reaction of melanogenesis, that is, conversion phenol to corresponding ortho-quinone. The enzyme from genus Streptomyces is generated as complex with "caddie" protein assists transport into center. In this complex, Tyr98 residue in caddie was found be accommodated pocket center tyrosinase, probably manner similar l-tyrosine genuine substrate tyrosinase. Under physiological conditions,...
Abstract We present an experimental and theoretical study of Raman spectroscopy isotopically pure diluted amorphous as well crystalline ices at 30 K. Our experiments comprise polarized (VV) depolarized (VH) spectra in the OH‐stretch region, whereas our calculations involve molecular dynamics (MD) simulation high‐ low‐density through vitrification subsequent formulation vibrational Hamiltonian coupled OH oscillators hydrogen‐bond network ices. VV VH each isotopic dilution are good agreement...
A new chromium(V)–oxo complex, [CrV(O)(6-COO–-py-tacn)]2+ (1; 6-COO–-py-tacn = 1-(6-carboxylato-2-pyridylmethyl)-4,7-dimethyl-1,4,7-triazacyclononane), was synthesized and characterized to evaluate the reactivity of CrV(O) complexes in a hydrogen-atom transfer (HAT) reaction by comparing it with that previously reported [CrV(O)(6-COO–-tpa)]2+ (2; 6-COO–-tpa N,N-bis(2-pyridylmethyl)-N-(6-carboxylato-2-pyridylmethyl)amine). Definitive differences these two were observed resonance Raman...
Objective: The SQ house dust mite (HDM) sublingual immunotherapy (SLIT)-tablet has demonstrated effective treatment of HDM-induced allergic asthma in patients 18 years or older European trials. This study investigated its safety and immunology profile Japanese adult with mild-to-moderate asthma. Methods: In this randomized, double-blind, placebo-controlled study, 48 were randomly assigned to a daily HDM SLIT-tablet placebo (3:1) for 14 d without an up-dosing regimen. Active groups comprised...
UV–visible absorption spectroscopy is useful for probing the electronic and structural changes of protein active sites, thus on-line combination X-ray diffraction spectroscopic analysis increasingly being applied. Herein, a novel spectrometer was developed at SPring-8 BL26B2 with nearly on-axis geometry between optical axes. A small prism mirror placed near beamstop to pass light only 2° off beam, enabling X-ray-exposed volume crystal during data collection. The applied NO reductase, heme...
Leaflet-specific lipid diffusion analysis revealed that polylysine adsorption on one leaflet of a bilayer is sensed by the opposite leaflet.
A lipid bilayer is a building block of cellular membranes. Understanding physicochemical properties and their composition dependence are thus inevitable to infer biological functions lipids...
This article is Open Access All publication charges for this have been paid by the Royal Society of Chemistry
Protein sample preparation is exactly the first step in every biophysical study.Curious proteins, however, are often difficult to be expressed by popular systems including E. coli and Pichia.Actin has been recognized as one of such challenging proteins.So far, employment various analytical methods requiring recombinant proteins impeded for studying actin.To overcome this issue, we have developing a new protein expression protocol with an inducible viral vector infection system...