Palmitoleic acid (PMA) has anti-inflammatory and antidiabetic activities. Here we tested whether these effects of PMA on glucose homeostasis liver inflammation, in mice fed with high-fat diet (HFD), are PPAR-α dependent. C57BL6 wild-type (WT) PPAR-α-knockout (KO) a standard (SD) or HFD for 12 weeks were treated after the 10th week oleic (OLA, 300 mg/kg b.w.) b.w. Steatosis induced by was associated inflammation only KO mice, as shown increased hepatic levels IL1-beta, IL-12, TNF-α; however,...

Background: Palmitoleic acid, since described as lipokine, increases glucose uptake by modulation of 5′AMP‐activated protein kinase (AMPK), well increasing lipolysis activation peroxisome proliferator‐activated receptor‐α (PPARα), in adipose tissue. However, liver, the effects palmitoleic acid on metabolism and role PPARα remain unknown. Objective: To investigate whether improved hepatic insulin sensitivity obese mice. Methods: C57BL6 knockout (KO) mice were fed for 12 weeks with a standard...

Abstract Probiotic supplementation arises as playing an immune-stimulatory role. High-intensity and -volume exercise can inhibit immune cell function, which threatens athletic performance recovery. We hypothesized that 30 days of probiotic could stabilize the system athletes preventing suppression after a marathon race. Twenty-seven male marathonists were double-blinded randomly into ( Bifidobacterium-animalis-subsp.-Lactis (10 × 10 9 ) Lactobacillus-Acidophilus + 5 g maltodextrin) placebo...

Background Every bout of exercise mobilizes and redistributes large numbers effector lymphocytes with a cytotoxic tissue migration phenotype. The frequent redistribution these cells is purported to increase immune surveillance play mechanistic role in reducing cancer risk slowing tumor progression physically active survivors. Our aim was provide the first detailed single cell transcriptomic analysis exercise-mobilized test their effectiveness as donor lymphocyte infusion (DLI) xenogeneic...

Abstract Marathon runners, subjected to intense training regimens and prolonged, exhaustive exercises, often experience a compromised immune response. Probiotic supplementation has emerged as potential remedy mitigate the impact of prolonged exercise on athletes. Consequently, this study sought assess influence probiotic monocyte functionality both before after official marathon race. Twenty-seven runners were randomly double-blindly assigned two groups: placebo ( n 13) (PRO) 14). Over 30 d,...

Nonalcoholic fatty liver disease (NAFLD) is one of the main diseases today, and may progress to steatohepatitis, cirrhosis, hepatocellular carcinoma. Some studies have shown beneficial effects aerobic exercise on reversing NAFLD. To verify whether chronic improves insulin resistance, inflammation, steatohepatitis caused by a high fat diet (HF) PPARα involved in these actions. C57BL6 wild type (WT) PPAR-α knockout (KO) mice were fed with standard (SD) or HF during 12 weeks; trained treadmill...

Abstract The aim of this study was to analyze the metabolic and molecular profile according physical fitness status (Low or High VO 2max ) its impacts on peripheral cellular inflammatory responses in healthy men. First ( Phase I) , (TNF-α, IL-6, IL-10) analyzed at baseline post-acute exercise sessions performed low (< 60% high (> 90% intensities considering individual endotoxin concentrations. Next II), monocyte cell cultures were treated with LPS alone associated Rosiglitazone (PPAR-γ...

Doxorubicin (DX) is a chemotherapeutic drug that used in clinical practice promotes deleterious side effects non-tumor tissues such as adipose tissue. We showed DX leads to extensive damage tissue via disruption 5'-adenosine monophosphate-activated protein kinase (AMPK) and PPAR-gamma signaling. Thus, we investigated whether co-treatment with the biguanide metformin (MET) could prevent of through activation AMPK The goal present study was verify adjuvant MET treatment subcutaneous (SAT)...

The aim of this study was to evaluate the inflammatory (peripheral and lipopolysaccharide (LPS)-stimulated released from whole blood) metabolic (glucose insulin) profile inactive obese men in response two isoenergetic models aerobic exercise training (~300 kcal each session). Twenty-two participants (28.7 ± 1.6 years; BMI = 34.4 0.1 kg/m2) were randomized into groups: I) HIIT: high-intensity interval (10× 1 bout: min - 100% Maximal Aerobic Velocity) or II) MICT: moderate-intensity continuous...

Evidence is emerging that exercise and physical activity provides protection against severe COVID-19 disease in patients infected with SARS-CoV-2, but it not known how affects immune responses to the virus. A healthy man completed a graded cycling ergometer test prior after SARS-CoV-2 infection, then again receiving an adenovirus vector-based vaccine. Using whole blood peptide stimulation assays, IFN-γ ELISPOT flow cytometry, ex vivo viral-specific T-cell expansion assays deep receptor (TCR)...

Athletes are advised to receive the COVID-19 vaccination protect themselves from SARS-CoV-2 infection during major competitions. Despite this, many athletes reluctant get vaccine due concerns that symptoms of vaccinosis may impair athletic performance. This study aimed determine effects on physiological responses graded exercise. Healthy physically active participants completed a 20-min bout cycling exercise at intensities corresponding 50%, 60%, 70%, and 80% predetermined V̇O

Exercise is a powerful tool for prevention and treatment of many conditions related to the cardiovascular system also chronic low-grade inflammation. Peroxisome proliferator-activated receptors γ (PPARγ) exerts an import role on regulation metabolic profile subsequent inflammatory response, especially in macrophages.To investigate effects 8-week moderate-exercise training parameters mice with PPARγ deficiency myeloid cells.Twelve-week old bearing deletion exclusively cells (PPARγlox/lox Lys...

Background Artistic Gymnastics is a sport where athletes are frequently fatigued. One element that might influence this aspect carbohydrate, an important energy substrate for the muscles and CNS. Our goal was to investigate of fatigue over artistic gymnastics athlete’s performance effects carbohydrate supplementation on their performance.Methods We evaluated 15 divided in 2 groups (control fatigue) from 12 14 years old two different experimental days. On first day (water day), they did 5...

Peroxisome proliferator‐activated receptors (PPARs) play a major role in metabolism and inflammatory control. Exercise can modulate PPAR expression skeletal muscle, adipose tissue, macrophages. Little is known about the effects of PPAR‐α metabolic profile cytokine secretion after acute exercise In this context, aim study was to understand influence on exercise‐mediated immune parameters peritoneal Mice C57BL/6 (WT) knockout (KO) were examined non‐exercising control ( n = 4) or 24 hours...

Circadian rhythm is essential for cellular regulation of physiological, metabolic, and immune functions. Perturbations circadian rhythms have been correlated with increased susceptibility to cancer poor prognosis in the treatment. Our aim investigate role doxorubicin (DOX) treatment on clock genes expression inflammation intraperitoneal macrophages antitumoral response.Macrophages were extracted from cavity mice without or Lewis lung carcinoma (LLC) treated DOX totaling four groups (CTL,...

Epidemiological data suggest that physical activity protects against severe COVID-19 and improves clinical outcomes, but how exercise augments the SARS-CoV-2 viral immune response has yet to be elucidated. Here we determine antigen-specific CD4 CD8 T-cell humoral immunity in non-vaccinated individuals with natural SARS CoV-2, using whole-blood peptide stimulation assays, IFN-γ ELISPOT 8-color flow cytometry, deep receptor (TCR) β sequencing, anti-RBD-1 neutralizing antibody serology. We...

CD3+/CD56+ Natural killer (NK) cell-like T-cells (NKT-like cells) represent <5% of blood lymphocytes, display a cytotoxic phenotype, and can kill various cancers. NKT-like cells be expanded ex vivo into cytokine-induced (CIK) cells, however this therapeutic cell product has had mixed results against hematological malignancies in clinical trials. The aim study was to determine if mobilized during acute cycling exercise could used generate more potent anti-tumor CIK from healthy donors. An...

Exercise mobilizes cytotoxic lymphocytes to blood which may allow superior cell products be harvested and manufactured for cancer therapy. Gamma-Delta (γδ) T-cells have shown promise treating solid tumors, but there is a need increase their potency against hematologic malignancies. Here, we show that human γδ mobilized in response just 20 minutes of graded exercise surface phenotypes transcriptomic profiles associated with cytotoxicity, adhesion, migration, cytokine signaling. Following 14...