A5043978878- Immune Cell Function and Interaction
- Exercise and Physiological Responses
- Congenital Heart Disease Studies
- Dermatology and Skin Diseases
- T-cell and B-cell Immunology
- Cancer, Stress, Anesthesia, and Immune Response
- Cardiac Arrhythmias and Treatments
- Cardiovascular Conditions and Treatments
- Cardiovascular Effects of Exercise
- Cardiac Arrest and Resuscitation
- Ultrasound in Clinical Applications
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- Pulmonary Hypertension Research and Treatments
- Hemodynamic Monitoring and Therapy
- Cardiac, Anesthesia and Surgical Outcomes
- Mechanical Circulatory Support Devices
- Vascular Procedures and Complications
- Healthcare Policy and Management
- Phonocardiography and Auscultation Techniques
- IL-33, ST2, and ILC Pathways
- Cardiac Structural Anomalies and Repair
- Cardiac pacing and defibrillation studies
- Central Venous Catheters and Hemodialysis
- Lysosomal Storage Disorders Research
University of Arizona
2014-2024
Vanderbilt University Medical Center
2024
University of Florence
2023
Washington University in St. Louis
2020-2022
St. Louis Children's Hospital
2022
University of Virginia Children's Hospital
2018-2019
University of Virginia
2017
Sudden cardiac arrest is a rare but devastating cause of death in young adults. Electrocardiograms may detect many causes sudden arrest, are not routinely included pre-athletic screening the United States America partly because high rates false-positive interpretation. To improve electrocardiogram specificity for identifying conditions associated with an expert panel developed refined criteria known as Seattle Criteria. Ours first study to compare standard Criteria 11- 13-year-olds. In...
Exercise mobilizes cytotoxic lymphocytes to blood which may allow superior cell products be harvested and manufactured for cancer therapy. Gamma-Delta (γδ) T-cells have shown promise treating solid tumors, but there is a need increase their potency against hematologic malignancies. Here, we show that human γδ mobilized in response just 20 minutes of graded exercise surface phenotypes transcriptomic profiles associated with cytotoxicity, adhesion, migration, cytokine signaling. Following 14...
Use of ultrasound (US) to facilitate vascular access has increased compared landmark-based procedures despite ergonomic challenges and need for extrapolation 2-dimensional images understand needle position. The MantUS™ system (Sentiar, Inc.,) uses a mixed reality (MxR) interface display US integrate real-time tracking.The purpose this prospective preclinical study was evaluate the feasibility usability MantUS in simulated environment.Participants were recruited from pediatric cardiology...
Background: The FUEL trial (Fontan Udenafil Exercise Longitudinal) demonstrated statistical improvements in exercise capacity following 6 months of treatment with udenafil (87.5 mg po BID). effect on echocardiographic measures single ventricle function this cohort has not been studied. Methods: 400 enrolled participants were randomized 1:1 to or placebo. Protocol echocardiograms obtained at baseline and 26 weeks after initiation udenafil/placebo. Linear regression compared change from...
Telemedicine is used with increasing frequency to improve patient care in remote areas. The interpretation of medical imaging on iPad(®) (Apple, Cupertino, CA) tablets has been reported be accurate. There are no studies the use iPads for pediatric echocardiograms. We compared quality echo images, diagnostic accuracy, and review time using three different modalities: access an iPad Air (iPad), via a computer (Remote), direct linked through Ethernet server, "gold standard" (Direct).Fifty...
Acyanotic congenital heart diseases or left-to-right shunting lesions are the most common form of disease. Although resolve spontaneously, many will remain hemodynamically significant, particularly in premature infant. Understanding difference pathophysiology, diagnosis, and management between term preterm infant is imperative to minimize risk secondary organ dysfunction ensure proper growth development.
<p>Interactions between DNAM-1 and PVR/Nectin-2 enhances the antileukemic activity of exercise expanded Vγ9Vδ2+ T-cells. <b>A,</b> Illustration receptors expressed on T-cells their corresponding ligands cancer cells. <b>B,</b> Heat map surface expression MFI K562 <b>C</b> <b>D,</b> The specific lysis cells by under two conditions: unstained control (−) respective blocking antibody (+), DNAM-1, PVR, Nectin-2, TRAIL, TRAIL-R1, TRAIL-R2...
<p>Acute exercise enhances the antileukemic activity of <i>ex vivo</i> expanded Vγ9Vδ2+ T-cells <i>in vitro</i> and vivo</i>. <b>A,</b> Schematic experimental design for experiments. <b>B,</b> The total number (1 × 10<sup>6</sup>) cells isolated before (REST) during final 5 minutes (EX) at day 0, generated in cell products after stimulation with ZOL+IL2 14 days. relative to γδ PBMC fractions 0. cellular composition...
<p>Supplemental Figure S1: The percentage or MFI of activating, inhibitory, and chemokine receptors among Vd2+ Vd1+ T-cells before during acute exercise</p>
<p>Exercise preferentially mobilizes γδ T-cells with phenotypic and transcriptomic profiles associated anti-tumor immunity. <b>A,</b> The percentage or MFI of activating, inhibitory, chemokine receptors among Vδ2+ Vδ1+ before (REST) during (EX) acute exercise (<i>n</i> = 8). Significance is indicated by * (<i>P</i> < 0.05). <b>B,</b> GSEA performed using KEGG GO terms showing enriched pathways on mobilized 3). Graphs show upregulated...
<p>Systemic β-AR activation through either acute exercise or ISO infusion evoke similar transcriptomic and surface phenotype shifts among <i>ex vivo</i> expanded Vγ9Vδ2+ T-cells. <b>A,</b> The percentage of activating, inhibitory, chemokine receptors T-cells before (REST) during (EX) infusion. (<i>n</i> = 8). Statistical significance set as *, <i>P</i> < 0.05. GSEA performed using KEGG GO terms showing enriched pathways on that were...
<p>Supplemental Figure S3: Schematic of the overall study design and conclusions.</p>
<p>The effect of acute exercise on lymphocyte subsets</p>
<p>ISO infusion enhances the antileukemic activity of <i>ex vivo expanded</i> Vγ9Vδ2+ T-cells <i>in vitro</i> and vivo</i>. <b>A,</b> Schematic experimental design for vivo</i> experiments. <b>B,</b> The total number (1 × 10<sup>6</sup>) cells isolated before (REST) during final 5 minutes ISO at day 0, generated in expanded cell products after stimulation with ZOL+IL2 14 days. relative to γδ PBMC fractions 0....
<p>Supplemental Figure S2: The specific lysis of K562 cells by expanded Vγ9Vδ2+ T-cells with respective blocking TCR-γδ or CMA.</p>
<p>ISO infusion enhances the antileukemic activity of <i>ex vivo expanded</i> Vγ9Vδ2+ T-cells <i>in vitro</i> and vivo</i>. <b>A,</b> Schematic experimental design for vivo</i> experiments. <b>B,</b> The total number (1 × 10<sup>6</sup>) cells isolated before (REST) during final 5 minutes ISO at day 0, generated in expanded cell products after stimulation with ZOL+IL2 14 days. relative to γδ PBMC fractions 0....
<p>Systemic β-AR activation through either acute exercise or ISO infusion evoke similar transcriptomic and surface phenotype shifts among <i>ex vivo</i> expanded Vγ9Vδ2+ T-cells. <b>A,</b> The percentage of activating, inhibitory, chemokine receptors T-cells before (REST) during (EX) infusion. (<i>n</i> = 8). Statistical significance set as *, <i>P</i> < 0.05. GSEA performed using KEGG GO terms showing enriched pathways on that were...
<div>Abstract<p>Exercise mobilizes cytotoxic lymphocytes to blood which may allow superior cell products be harvested and manufactured for cancer therapy. Gamma-Delta (γδ) T-cells have shown promise treating solid tumors, but there is a need increase their potency against hematologic malignancies. Here, we show that human γδ mobilized in response just 20 minutes of graded exercise surface phenotypes transcriptomic profiles associated with cytotoxicity, adhesion, migration,...
<p>Supplemental Figure S3: Schematic of the overall study design and conclusions.</p>
<p>Acute exercise enhances the antileukemic activity of <i>ex vivo</i> expanded Vγ9Vδ2+ T-cells <i>in vitro</i> and vivo</i>. <b>A,</b> Schematic experimental design for experiments. <b>B,</b> The total number (1 × 10<sup>6</sup>) cells isolated before (REST) during final 5 minutes (EX) at day 0, generated in cell products after stimulation with ZOL+IL2 14 days. relative to γδ PBMC fractions 0. cellular composition...
<p>Exercise preferentially mobilizes γδ T-cells with phenotypic and transcriptomic profiles associated anti-tumor immunity. <b>A,</b> The percentage or MFI of activating, inhibitory, chemokine receptors among Vδ2+ Vδ1+ before (REST) during (EX) acute exercise (<i>n</i> = 8). Significance is indicated by * (<i>P</i> < 0.05). <b>B,</b> GSEA performed using KEGG GO terms showing enriched pathways on mobilized 3). Graphs show upregulated...
<p>Blockade of β<sub>2</sub>-AR blunts the mobilization and expansion Vγ9Vδ2+ T-cells. <b>A,</b> The physiologic effects a placebo, β1-AR antagonist (10 mg bisoprolol), or nonselective β1+β2 (80 mg, nadolol) on VO<sub>2</sub> (mL/minute), systolic blood pressure (mmHg), HR (bpm), epinephrine (ng/mL), norepinephrine (ng/mL) during last minutes acute exercise (<i>n</i> = 9). antagonist, total number T-cells mobilized with...
<p>Supplemental Figure S2: The specific lysis of K562 cells by expanded Vγ9Vδ2+ T-cells with respective blocking TCR-γδ or CMA.</p>