A5064577517- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Meat and Animal Product Quality
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Animal Nutrition and Physiology
- Diet and metabolism studies
- Identification and Quantification in Food
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Food Quality and Safety Studies
Shanghai Academy of Agricultural Sciences
2022-2025
Ministry of Agriculture and Rural Affairs
2022-2025
Biotechnology Research Institute
2022-2023
To study the differences in meat quality and nutritional components between local Shanghai pig breeds Meishan (MS), Shawutou (SWT), Fengjing (FJ), commercial Duroc × Landrace Yorkshire (DLY) crossbred pigs, to provide data support for selection breeding of superior breeds, this selected 30 piglets each three DLY with similar birth ages weights around 25 kg, fed them same daily ration uniform components, slaughtered ten at 100 kg weight evaluation indicators (primarily intramuscular fat...
Rapid, accurate and visual point-of-care testing (POCT) methods for pathogenic bacteria detection are essential avoiding foodborne diseases caused by pathogens or their toxins. In this study, we proposed a rapid method that named “Cas12aVIP”. By combining recombinase polymerase amplification (RPA), CRISPR/Cas12a system cationic-conjugated polythiophene derivative (poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrochloride] (PMNT) mixed with single-stranded DNA...
With the increasing acreage of genetically modified crops worldwide, rapid and efficient detection technologies have become very important for regulation screening GM organisms. We constructed a method based on loop-mediated isothermal amplification (LAMP), CRISPR-Cas12a lateral flow assay (LAMP-CRISPR-Cas12a-LFA). It is an intuitive, sensitive specific fluorescence test strip system to detect CP4-EPSPS Cry1Ab/Ac genes in field screening. The LAMP-CRISPR-Cas12a-LFA has limit (LOD) 100 copies...
In order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) specific genomic regions from five selected species specificity amplification efficiency determined. The mixed DNA template an equal copy number was used detecting the accuracy limit multiplex PCR. results showed that probes had good minimum detection copies: 0.15 copies/µL (Bos taurus), 0.28 copies/μL duck...
Luchuan (LC) pigs are a Chinese breed renowned for their distinctive black and white coloring, superior meat quality rapid reproduction, but growth rate is slow. Over the course of approximately two decades controlled breeding, LC maintained at Shanghai Academy Agricultural Sciences (Shanghai, China) have diverged into phenotypes: one characterized by obesity (FLC) other leanness (LLC). Recent studies indicate correlation between microorganisms differentiation host phenotypes. In this study,...