A5032899856- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Fungal Biology and Applications
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Genetically Modified Organisms Research
- Vibrio bacteria research studies
- Identification and Quantification in Food
- Urological Disorders and Treatments
- Pediatric Urology and Nephrology Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Viral gastroenteritis research and epidemiology
- Plant biochemistry and biosynthesis
- Microbial Community Ecology and Physiology
- Transgenic Plants and Applications
- Food composition and properties
- Mycorrhizal Fungi and Plant Interactions
- Phytochemicals and Antioxidant Activities
- Urinary Tract Infections Management
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Phytase and its Applications
- Molecular Biology Techniques and Applications
Shanghai Academy of Agricultural Sciences
2014-2024
Qingdao University
2022-2024
Ministry of Agriculture and Rural Affairs
2019-2024
Biotechnology Research Institute
2011-2024
Qingdao Women and Children's Hospital
2023-2024
Anhui Agricultural University
2010-2024
Changsha Medical University
2024
XinHua Hospital
2023
Shanghai Jiao Tong University
2016-2023
Lanzhou University of Technology
2023
The phytohormone salicylic acid (SA) plays essential roles in biotic and abiotic responses, plant development, leaf senescence. 2,5-Dihydroxybenzoic (2,5-DHBA or gentisic acid) is one of the most commonly occurring aromatic acids green plants assumed to be generated from SA, but enzymes involved its production remain obscure. DMR6 (Downy Mildew Resistant6; At5g24530) has been proven immunity Arabidopsis (Arabidopsis thaliana), biochemical properties are not well understood. Here, we report...
The environmental impacts of genetically modified (GM) plants remain a controversial global issue. To address these issues, comprehensive risk assessments GM is critical for the sustainable development and application transgenic technology. In this paper, significant differences were not observed between microbial metagenomic metabolomic profiles in surface waters Bt rice (T1C-1, line) non-Bt cultivars (Minghui 63 (the isogenic Zhonghua 11 conventional japonica cultivar)). contrast, apparent...
The salty oligopeptides from
Through virtual screening, electronic tongue verification, and molecular docking technology, the structure-taste activity relationship of 47 kinds umami peptides (octapeptide - undecapeptide) from Stropharia rugosoannulata prepared by simultaneous ultrasonic-assisted directional enzymatic hydrolysis was analyzed. The S.rugosoannulata can form hydrogen bond interaction electrostatic with receptors T1R1/T1R3. amino acid residues at peptides' N-terminal C-terminal play a vital role in binding...
Salicylic acid (SA) is a crucial hormone involved in plant immunity. Rice (Oryza sativa) maintains high SA levels that are not induced by pathogens. However, the roles of rice immunity and yield remain largely unknown. Here, we identified 5-hydroxylases 1 (OsS5H1) 2 (OsS5H2) as primary enzymes engaged catalysing to 2,5-dihydroxybenzoic (2,5-DHBA) rice. were significantly increased oss5h mutants, while they dramatically decreased OsS5H1 OsS5H2 overexpression lines. The mutants resistant,...
Abstract Purpose Plant growth–promoting (PGP) bacteria are an environment-friendly alternative to chemical fertilizers for promoting plant growth and development. We isolated characterized a PGP endophyte, YSD YN2, from the leaves of Cyperus esculentus L. var. sativus . Methods Specific characteristics this strain, such as phosphate solubilization ability, potassium-dissolving siderophore indole-3-acetic acid (IAA) production, salt tolerance, were determined in vitro. In addition, positive...
The aim of this study was to evaluate the effect microencapsulated essential oils (MEO) on laying performance, egg quality, immunity, intestinal morphology, and oxidative status hens. A total 640 Hy-line Brown hens, 41 wk age, were randomly divided into 4 groups, each with 8 replicates containing 20 birds per replicate. dietary conditions tested included a basal diet (Control) or supplemented various levels MEO at 100 mg/kg (MEO100), 300 (MEO300), 500 (MEO500). three treatment groups...
Abstract To identify the characteristic taste components of common cultivated mushroom (brown; Portobello), Agaricus bisporus , in stipe and pileus Portobello harvested at different growth stages were extracted identified, principal component analysis (PCA) active value (TAV) used to reveal during each mushroom. In pileus, 20 14 respectively, they considered as fruit bodies, which included most amino acids 5′‐nucleotides. Some that found high levels, such lactic acid citric acid, not...
Undecapeptide is the central peptide molecule in base material of Stropharia rugosoannulata, and angiotensin-converting enzyme (ACE) plays a crucial role hypertension. To fully explore interaction mechanism ACE-inhibitory activity long-chain peptides from binding conformations twenty-seven undecapeptides with ACE receptor were revealed by docking. The undecapeptide GQEDYDRLRPL better capacity higher secondary mass spectral abundance was screened. All amino acid residues except proline...
Staphylococcus aureus exists widely in the natural environment and is one of main food-borne pathogenic microorganisms causing human bacteremia. For safe food management, a rapid, high-specificity, sensitive method for detection S. should be developed. In this study, platform detecting (nuc gene) based on isothermal amplification (loop-mediated amplification-LAMP, recombinase polymerase amplification-RPA) clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated...
In contrast to other transgenic Bacillus thuringiensis (Bt) crops (e.g. Bt maize and cotton), risk assessments of rice on soil ecosystem are few. To assess the influence rhizosphere ecosystems, samples from Bt, non-Bt controls were taken at seedling, tillering, booting, heading maturing stages. The activities dehydrogenases, invertase, phenol oxidases, acid phosphatases, ureases proteases showed no significant differences between rice. A Biolog system was used evaluate effect functional...
Abstract Aroma is an important factor affecting mushroom character and quality. According to the different reaction pathway, key aroma metabolites (sulfur eight‐carbon volatiles) formation can be classified into enzymatic reactions nonenzymatic reactions. volatiles are generated from precursors via biocatalytic activities of various synthases during growth stages shiitake mushrooms. Understanding specific relationships between their improving At same time, reduce forest logging burning...
Traditional transgenic detection methods require high test conditions and struggle to be both sensitive efficient. In this study, a one-tube dual RPA reaction system for <i>CP4-EPSPS</i> <i>Cry1Ab/Ac</i> was proposed combined with lateral flow immunochromatographic assay, named "Dual-RPA-LFD", visualize the of GM crops. which, herbicide tolerance gene insect resistance were selected as targets taking into account current status most widespread application traits their stacked traits....
Abstract Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture recent decades. Rapid detection of PCV2 is very important for effective prophylaxis treatment PMWS. To establish a sensitive, specific assay quantitation PCV2, we designed synthesized primers probe open reading frame 2. The had wide dynamic range with excellent linearity reliable reproducibility, detected between 10 copies...
Abstract BACKGROUND Metabolite formation is a physiological stress response during the growth and development of shiitake mushrooms ( Lentinula edodes ). The characteristic flavor metabolites are important quality components in mushrooms. To investigate mechanisms metabolites, transcriptome analyses were performed on harvested at different stages. RESULTS In total, 30 genes related to synthesis volatiles identified via screening. Through KEGG (Kyoto Encyclopedia Genes Genomes) enrichment...