A5068183875- Ginger and Zingiberaceae research
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Animal Virus Infections Studies
- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Neurobiology and Insect Physiology Research
- Plant biochemistry and biosynthesis
- Virus-based gene therapy research
- Silkworms and Sericulture Research
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Insect and Arachnid Ecology and Behavior
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Viral Infections and Immunology Research
- Insect Utilization and Effects
- RNA Interference and Gene Delivery
- Garlic and Onion Studies
- Plant Gene Expression Analysis
- Plant responses to water stress
- Yersinia bacterium, plague, ectoparasites research
Chongqing University of Arts and Sciences
2015-2024
Southwest University
2007-2016
State Key Laboratory of Silkworm Genomic Biology
2013-2016
Chongqing University
2014
Shanghai Academy of Agricultural Sciences
2007-2013
Abstract Ginger (Zingiber officinale), the type species of Zingiberaceae, is one most widespread medicinal plants and spices. Here, we report a high-quality, chromosome-scale reference genome ginger ‘Zhugen’, traditionally cultivated in Southwest China used as fresh vegetable, assembled from PacBio long reads, Illumina short high-throughput chromosome conformation capture (Hi-C) reads. The was phased into two haplotypes, haplotype 1 (1.53 Gb with contig N50 4.68 M) 0 (1.51 5.28 M)....
The silkworm Bombyx mori is a lepidopteran insect with four developmental stages: egg, larva (caterpillar), pupa, and adult. hemolymph of the in an open system that circulates among all organs, functions nutrient hormone transport, injury, immunity. To understand intricate mechanisms metamorphosis, from different stages, including 3rd day fifth instar, 6th pupation, 8th pupal stage first moth stage, was investigated by two-dimensional electrophoresis mass spectrometry.Two-dimensional...
Porcine epidemic diarrhea virus (PEDV) causes severe economic losses in the swine industry China and other Asian countries. Infection usually leads to an acute, often lethal piglets. Despite impact of disease, no system is yet available manipulate viral genome which has severely hampered research on this until today. We have established a reverse genetics for PEDV based targeted RNA recombination that allows modification 3′-end genome, encodes structural proteins ORF3 protein. Using system,...
Abstract Background AP2/ERF transcription factors (TFs) constitute one of the largest TF families in plants, which play crucial roles plant metabolism, growth, and development as well biotic abiotic stresses responses. Although family has been thoroughly identified many species several TFs have functionally characterized, little is known about this ginger ( Zingiber officinale Roscoe), an important affinal drug diet vegetable. Recent completion genome sequencing provides opportunity to...
Ginger (Zingiber officinale Rosc.) is a popular flavoring that widely used in Asian, and the volatile oil ginger rhizomes adds special fragrance taste to foods. The bioactive compounds ginger, such as gingerols, diarylheptanoids, flavonoids, are of significant value human health because their anticancer, anti-oxidant, anti-inflammatory properties. However, non-model plant, knowledge about genome sequences extremely limited, this limits molecular studies on plant. In study, de novo...
The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by middle and posterior glands. In view of significant role ASG in liquid–crystal spinning process, shotgun proteomics approach was taken to study relationship between function proteins silkworm mechanism. A total 1132 with 7647 unique peptides were identified dataset including some involved cuticle, ion transportation, energy metabolism, apoptosis. Two putative cuticle-specific highly...
Abstract Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture recent decades. Rapid detection of PCV2 is very important for effective prophylaxis treatment PMWS. To establish a sensitive, specific assay quantitation PCV2, we designed synthesized primers probe open reading frame 2. The had wide dynamic range with excellent linearity reliable reproducibility, detected between 10 copies...
Ginger ( Zingiber officinale Roscoe), a perennial herb, is one of the most economically valuable plants in Zingiberaceae family. Gingerol, as major constituents ginger essential oil, contributes to unique flavor and pharmaceutical value ginger. However, pathway gingerol biosynthesis has not been verified described help understand secondary metabolites nonmodel species. In this study, concentrations gingerols were quantified at different stages rhizome development tissues. The results...
Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS), and porcine dermatitis nephropathy (PDNS). It has caused heavy losses in global agriculture recent decades. Rapid detection PCV2 very important for effective prophylaxis treatment PMWS.A loop-mediated isothermal amplification (LAMP) assay was used to detect this study. Three pairs primers were specially designed recognizing eight distinct sequences ORF2 gene. This gene lies virus...
The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion protects gut epithelium. We used gel electrophoresis mass spectrometry to identify extracted proteins from silkworm PM obtain an in-depth understanding of biological function components. A total 305 proteins, molecular weights ranging 8.02 kDa 788.52 isoelectric points 3.39 12.91, were successfully identified. also found several major...
Eucalyptus dunnii is an important macrophanerophyte with high economic value. However, low temperature stress limits its productivity and distribution. To study the cold response mechanisms of E. dunnii, 5 cDNA libraries were constructed from mRNA extracted leaves exposed to for varying lengths time evaluated by RNA-Seq analysis. The assembly Illumina datasets was optimized using various programs parameters. final generated 205,325 transcripts average length 1,701 bp N50 2,627 bp,...
Alternaria alternata is a pathogen in wide range of agriculture crops and causes significant economic losses. A strain A. (Y784-BC03) was isolated identified from “Hongyang” kiwifruit demonstrated to cause black spot infections on fruits. The genome sequence Y784-BC03 obtained using Nanopore MinION technology. assembled composed 33,869,130bp (32.30Mb) comprising 10 chromosomes 11,954 genes. total 2,180 virulence factors were predicted be present the sequence. comprised genes encoding...
Bacterial infections are the cause of rhizome rot in ginger (Zingiber officinale). Key members endophytic microbial community rhizomes have not been identified, and their impact on decay during activation adventitious bud development has investigated. High-throughput, 16S rRNA amplicon sequencing inoculation experiments were used to analyze diversity, structure composition, pathogenicity isolated bacteria. Our results indicated that composition microbiota underwent a shift progression...
Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion osmoregulation. In order to highlight the important genes pathways involved in multi-functions MTs, we performed a systematic proteomic analysis silkworm MTs present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, as well Trans Proteomic Pipeline (TPP) Absolute protein expression...
Sericin is a kind of water-soluble protein expressed specifically in the middle silk gland Bombyx mori. When sericin-1 gene promoter was cloned and transgenic vector constructed to express foreign protein, specific Helitron, Bmhel-8, identified sequence some genotypes mori mandarina. Given that Bmhel-8 Helitron transposon present only genotypes, it could be source allelic variation promoter. The length approximately 1063 or 643 bp. larger size allele ascribed presence Bmhel-8. Silkworm can...
Abstract The silkworm, Bombyx mori , is an economically important insect with a 5 000‐year history of domestication. During evolution, the silkworm has developed highly effective defenses against invasion and parasitization by microorganisms. In this study, two microorganisms Escherichia coli Bacillus bombyseptieus were orally infected to larvae. After infection E. B. for 24 h, we investigated polypeptide changes in hemolymph, midgut integument using two‐dimensional gel electrophoresis...