A5047542570- Advanced NMR Techniques and Applications
- Electron Spin Resonance Studies
- Advanced MRI Techniques and Applications
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Atomic and Subatomic Physics Research
- Brain Tumor Detection and Classification
- Lower Extremity Biomechanics and Pathologies
- Balance, Gait, and Falls Prevention
- Cardiomyopathy and Myosin Studies
- Molecular spectroscopy and chirality
- Neonatal Health and Biochemistry
- Mitochondrial Function and Pathology
- Physiological and biochemical adaptations
- Metabolism, Diabetes, and Cancer
- NMR spectroscopy and applications
- Diet and metabolism studies
- Viral Infectious Diseases and Gene Expression in Insects
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Radiomics and Machine Learning in Medical Imaging
- Muscle activation and electromyography studies
- Genetics, Aging, and Longevity in Model Organisms
Enzo Life Sciences (United States)
2022
École Polytechnique Fédérale de Lausanne
2013-2022
Hacettepe University
2009
Various tumors develop addiction to glutamine support uncontrolled cell proliferation. Here we identify the nuclear receptor liver homolog 1 (LRH-1) as a key regulator in process of hepatic tumorigenesis through coordination noncanonical pathway that is reliant on mitochondrial and cytosolic transaminases glutamate pyruvate transaminase 2 (GPT2) oxaloacetate (GOT1), which fuel anabolic metabolism. In particular, show gain loss function LRH-1 modulate expression activity glutaminase (GLS2),...
Greater than 60% solution-state polarization of [1-<sup>13</sup>C]pyruvic acid polarized at 7 T and 1.0 K can be measured after rapid transfer to an MRI scanner magnet.
Abstract The prevalence of cardiovascular disease increases exponentially with age, highlighting the contribution aging mechanisms to cardiac diseases. Although model organisms which share human pathologies can elucidate driving disease, they do not provide us innate examples how might be slowed or attenuated. identification animal models that preserve function throughout most life offers an alternative approach study slow aging. One such species may naked mole-rat (NMR), a mouse-sized (40...
Abstract The γ‐glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis several pathologies. We propose new molecular probe, γ‐Glu‐[1‐ 13 C]Gly, monitoring vivo by hyperpolarized (HP) C magnetic resonance (MR). properties C]Gly are suitable HP metabolic analysis since chemical shift between and its product, [1‐ is large (4.3 ppm) T 1 both compounds relatively long (30 s 45 s,...
The metabolic shift induced in human CD4+ T lymphocytes by stimulation is characterized an upregulation of glycolysis, leading to augmentation lactate production. This adaptation has already been highlighted with various techniques and reported several previous studies. We herein propose a method rapidly noninvasively detect the associated increase flux from pyruvate catalyzed dehydrogenase using hyperpolarized 13C magnetic resonance, technique which can be used for vivo imaging. It was...
Abstract Hyperpolarized [1- 13 C]pyruvate enables direct in vivo assessment of real-time liver enzymatic activities by C magnetic resonance. However, the technique usually requires injection a highly supraphysiological dose pyruvate. We herein demonstrate that metabolism can be measured with hyperpolarized administered at two- to three-fold basal plasma concentration. The flux through pyruvate dehydrogenase, assessed C-labeling bicarbonate fed condition, was found saturated or partially...
Abstract The γ‐glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis several pathologies. We propose new molecular probe, γ‐Glu‐[1‐ 13 C]Gly, monitoring vivo by hyperpolarized (HP) C magnetic resonance (MR). properties C]Gly are suitable HP metabolic analysis since chemical shift between and its product, [1‐ is large (4.3 ppm) T 1 both compounds relatively long (30 s 45 s,...
NAD+ is required for vital cellular processes such as redox regulation, DNA damage repair and cell signaling. There has been a growing interest in modifying concentrations the hope that it might increase longevity. However, there no clear consensus on changes concentration due to healthy aging. Commonly used vitro assays NAD quantification are not suitable longitudinal studies. We therefore implemented an vivo downfield 1H MRS method characterize during aging mice showed can detect hypoxia...
This paper represents an artificial neural network that captures knee angle variations for adult gait scenarios. Back propagation algorithm is used to train the network. The data set are needed training have been obtained artificially. Gait cycle analysed in eight different phases. With model, phase and subsequent value predicted suggested model trained inclinations walking speed, results recorded discussed.
Dysregulation of cellular metabolism is a key feature major diseases such as diabetes and cancer. Monitoring metabolic alterations critical importance for improved understanding disease progression the development novel therapeutics. Carbon-13 magnetic resonance spectroscopy (13C MRS), which can detect quantitate metabolites in non-invasive manner, well suited clinical pre-clinical studies. The low natural abundance 13C allows one to trace exogenously administered 13C-labeled substrates by...