A5070414794- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Solid-state spectroscopy and crystallography
- NMR spectroscopy and applications
- Atomic and Subatomic Physics Research
- Nuclear Physics and Applications
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Radiation Detection and Scintillator Technologies
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Photochemistry and Electron Transfer Studies
- Particle Detector Development and Performance
- Meat and Animal Product Quality
- Identification and Quantification in Food
- Molecular spectroscopy and chirality
- Metal and Thin Film Mechanics
- High-pressure geophysics and materials
- Photosynthetic Processes and Mechanisms
- Viral gastroenteritis research and epidemiology
- Biochemical effects in animals
- Diamond and Carbon-based Materials Research
Technical University of Denmark
2016-2025
Ørsted (Denmark)
2017-2023
Institut Néel
2020
European Spallation Source
2020
Aarhus University
2020
Université Grenoble Alpes
2020
Lawrence Berkeley National Laboratory
2020
Centre National de la Recherche Scientifique
2020
Resonance Research (United States)
2016-2019
Robert Bosch (Germany)
2019
A method for obtaining strongly polarized nuclear spins in solution has been developed. The uses low temperature, high magnetic field, and dynamic polarization (DNP) to polarize the solid state. sample is subsequently dissolved rapidly a suitable solvent create of molecules with hyperpolarized spins. performed DNP polarizer, consisting super-conducting magnet (3.35 T) liquid-helium cooled space. irradiated microwaves at approximately 94 GHz. Subsequent polarization, by an injection system...
The "Warburg effect," an elevation in aerobic glycolysis, may be a fundamental property of cancer cells. For diagnosis and treatment, it would valuable if elevated glycolytic metabolism could quantified image animals humans. pyruvate molecule is at the metabolic crossroad for energy delivery inside cell, with noninvasive measurement relative transformation into lactate alanine within biologically relevant time frame (seconds), possible to quantify status We have examined after i.v. injection...
Dynamic nuclear polarization of (13)C-labeled cell substrates has been shown to massively increase their sensitivity detection in NMR experiments. The gain is sufficiently large that if these polarized molecules are injected intravenously, spatial distribution and subsequent conversion into other metabolites can be imaged. We have used this method image the fumarate malate a murine lymphoma tumor vivo after i.v. injection hyperpolarized [1,4-(13)C(2)]fumarate. In isolated cells, rate labeled...
Abstract Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of 13 C MR spectroscopy (MRS). [5‐ 1 ]Glutamine was hyperpolarized using this by up to 5%, representing a 6000‐fold increase in sensitivity. The conversion glutamine glutamate mitochondrial glutaminase demonstrated C‐MRS measurements cultured human hepatoma cells (HepG2). These results represent first step developing imaging detecting metabolism vivo. Furthermore, since utilization has been...
An overview is provided of the 15 neutron beam instruments making up initial instrument suite European Spallation Source (ESS), and being made available to user community. The ESS source consists a high-power accelerator target station, providing unique long-pulse time structure slow neutrons. design considerations behind structure, moderator geometry layout are presented. 15-instrument two small-angle instruments, reflectometers, an imaging beamline, single-crystal diffractometers; one for...
Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility reactions and reaction mechanisms in vivo with subsecond resolution.
Greater than 60% solution-state polarization of [1-<sup>13</sup>C]pyruvic acid polarized at 7 T and 1.0 K can be measured after rapid transfer to an MRI scanner magnet.
The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used study cerebral metabolism, 13C magnetic resonance spectroscopy (MRS) allows following fate of 13C-enriched substrates through pathways. We herein demonstrate that it is possible measure metabolism in vivo with sub-second time resolution using hyperpolarized MRS. In particular, dynamic 13C-labeling pyruvate and lactate formed from 13C-glucose was observed real time....
<title>Abstract</title> Hyperpolarized Magnetic Resonance Imaging (HypMRI) offers valuable insights into dynamic physiological processes <italic>in vivo</italic>. We introduce hyperpolarized <sup>15</sup>N,d<sub>9</sub>-Betaine (trimethyl-<sup>2</sup>H<sub>9</sub>-<sup>15</sup>N-glycine) as an endogenous MRI contrast agent with a long-lived signal suited for comprehensive molecular tracking. With detectable vivo</italic> lasting more than 14 minutes and high polarization efficiency, supports...
Abstract The structure of a nonspecific lipid transfer protein from barley (ns‐LTP ) in complex with palmitate has been determined by NMR spectroscopy. compared to the ns‐LTP absence palmitate, palmitoyl coenzyme A, ofns‐LTP maize its free form, and complexed palmitate. Binding only affects moderately contrast binding which leads considerable expansion protein. modes are different. Although neither case there major conformational changes protein, orientation two proteins is exactly opposite.
Abstract Powerful analytical tools are vital for characterizing the complex molecular changes underlying oncogenesis and cancer treatment. This is particularly true, if information to be collected in vivo by noninvasive approaches. In recent past, hyperpolarized 13 C magnetic resonance (MR) spectroscopy has been employed quickly collect detailed spectral on chemical fate of tracer molecules different tissues at high sensitivity. Here, we report a preclinical study showing that...
Abstract Dynamic nuclear polarization can be used to increase the sensitivity of solution state 13 C magnetic resonance spectroscopy by four orders magnitude. We show here that [1‐ C]glutamate polarized 28%, representing a 35,000‐fold in its detection at 9.4 T and 37°C. The metabolism hyperpolarized glutamate α‐ketoglutarate, catalyzed enzyme alanine transaminase, was detected vitro human hepatoma cells (HepG2). Incubation with sodium pyruvate increased level label α‐ketoglutarate pool, an...
The promising dynamic nuclear polarization (DNP) for hyperpolarized 13 C‐MRI/MRS of real‐time metabolism in vivo is challenged by the limited number agents with required physical and biological properties. requirement a liquid‐state T 1 tens seconds mostly found C‐carbons small molecules that have no direct protons attached, i.e. carbonyl, carboxyl certain quaternary carbons. Unfortunately, such carbon positions do not exist large metabolic agents, chemical shift dispersion often limits...