A5051798578- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Atomic and Subatomic Physics Research
- Solid-state spectroscopy and crystallography
- NMR spectroscopy and applications
- Enzyme Structure and Function
- Lanthanide and Transition Metal Complexes
- Medical Imaging Techniques and Applications
- Radio Frequency Integrated Circuit Design
- Superconducting Materials and Applications
- Metabolomics and Mass Spectrometry Studies
- Particle accelerators and beam dynamics
- VLSI and Analog Circuit Testing
- Ultrasound and Hyperthermia Applications
- Particle Accelerators and Free-Electron Lasers
- Gyrotron and Vacuum Electronics Research
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- Photochemistry and Electron Transfer Studies
- Integrated Circuits and Semiconductor Failure Analysis
- Analog and Mixed-Signal Circuit Design
- Molecular spectroscopy and chirality
- Physics of Superconductivity and Magnetism
- Acoustic Wave Resonator Technologies
Technical University of Denmark
2015-2024
Ørsted (Denmark)
2015-2023
Hvidovre Hospital
2012-2019
Copenhagen University Hospital
2012-2019
General Electric (Spain)
2008-2019
General Electric (Denmark)
2012-2019
Resonance Research (United States)
2015-2019
Robert Bosch (Germany)
2019
General Electric (Canada)
2019
Capital Region of Denmark
2017
A method for obtaining strongly polarized nuclear spins in solution has been developed. The uses low temperature, high magnetic field, and dynamic polarization (DNP) to polarize the solid state. sample is subsequently dissolved rapidly a suitable solvent create of molecules with hyperpolarized spins. performed DNP polarizer, consisting super-conducting magnet (3.35 T) liquid-helium cooled space. irradiated microwaves at approximately 94 GHz. Subsequent polarization, by an injection system...
Metabolic imaging with hyperpolarized pyruvate was used to safely and noninvasively visualize prostate tumors in patients.
The "Warburg effect," an elevation in aerobic glycolysis, may be a fundamental property of cancer cells. For diagnosis and treatment, it would valuable if elevated glycolytic metabolism could quantified image animals humans. pyruvate molecule is at the metabolic crossroad for energy delivery inside cell, with noninvasive measurement relative transformation into lactate alanine within biologically relevant time frame (seconds), possible to quantify status We have examined after i.v. injection...
Dynamic nuclear polarization has enabled hyperpolarization of nuclei such as 13C and 15N in endogenous substances. The resulting high makes it possible to perform subsecond MRI. By using the dynamic technique, 10% was obtained an aqueous solution 100 mM 13C-labeled urea, ready for injection. vivo T1 relaxation time urea determined 20 +/- 2 s. Due long time, is use hyperpolarized substance medical imaging. A series high-resolution ( approximately 1-mm) magnetic resonance images were acquired,...
A novel polarizer based on the dissolution‐dynamic nuclear polarization (DNP) method has been designed, built and tested. The differs from those previously described by being designed with sterile use intent compatible clinical use. main features are: (1) an integral, disposable fluid path containing all pharmaceuticals constituting a barrier, (2) closed‐cycle cryogenic system to eliminate consumption of liquid cryogens (3) multi‐sample increase throughput. consists vial agent be polarized,...
An efficient noninvasive method for in vivo imaging of tumor oxygenation by using a low-field magnetic resonance scanner and paramagnetic contrast agent is described. The methodology based on Overhauser enhanced (OMRI), functional technique. OMRI experiments were performed tumor-bearing mice (squamous cell carcinoma) i.v. administration the Oxo63 (a highly derivatized triarylmethyl radical) at nontoxic doses range 2-7 mmol/kg either as bolus or continuous infusion. Spatially resolved pO(2)...
Purpose A novel dissolution dynamic nuclear polarization (dDNP) polarizer platform is presented. The meets a number of key requirements for in vitro, preclinical, and clinical applications. Method It uses no liquid cryogens, operates continuous mode, accommodates wide range sample sizes up to including those required human studies, fully automated. Results offers operational window both terms magnetic field, 10.1 T, temperature, from room temperature down 1.3 K. delivers 13 C state [1‐...
Abstract Purpose: To demonstrate dynamic imaging of a diffusible perfusion tracer, hyperpolarized [ 13 C]urea, for regional measurement blood flow in preclinical cancer models. Materials and Methods: A pulse sequence using balanced steady state free precession (bSSFP) was developed, with progressively increasing flip angles efficient sampling the magnetization. This allowed temporal volumetric C]urea signal. Regional signal dynamics were quantified kidneys liver, estimates relative flows...
Dynamic nuclear polarization (DNP) has evolved as the method of choice to enhance NMR signal intensities and address a variety otherwise inaccessible chemical, biological physical questions. Despite its success, there is no detailed understanding how large electron transferred surrounding nuclei or where these are located relative polarizing agent. To questions we perform an analysis three-spin solid effect, show that it exquisitely sensitive electron-nuclear distances. We exploit this...
The strong enhancement of NMR signals achieved by hyperpolarization decays, at best, with a time constant few minutes. Here, we show that combination long-lived singlet states, molecular design, magnetic field cycling, and specific radiofrequency pulse sequences allows repeated observation the same batch polarized nuclei over period 30 min more. We report recycling protocol in which enhanced nuclear polarization dissolution-DNP is observed full intensity then returned to order. MRI...
Abstract Background This experimental study explores a novel magnetic resonance imaging/spectroscopic (MRI/MRS) method that measures changes in renal metabolism diabetic rat model. hyperpolarized metabolic MRI/MRS allows monitoring of processes seconds by >10 000‐fold enhancement the MR signal. The has shown conversion pyruvate to bicarbonate, i.e. dehydrogenase (PDH) activity, is significantly altered myocardium already at onset diabetes, and predominant Warburg effect valuable cancer...
Abstract Within the last decade hyperpolarized [1‐ 13 C] pyruvate chemical‐shift imaging has demonstrated impressive potential for metabolic MR a wide range of applications in oncology, cardiology, and neurology. In this work, highly efficient pulse sequence is described time‐resolved, multislice chemical shift injected substrate obtained downstream metabolites. Using spectral‐spatial excitation combination with single‐shot spiral data acquisition, overall encoding evenly distributed between...
Abstract Metabolic reprogramming is one of the defining features cancer and abnormal metabolism associated with many other pathologies. Molecular imaging techniques capable detecting such changes have become essential for diagnosis, treatment planning, surveillance. In particular, 18 F-FDG (fluorodeoxyglucose) PET has emerged as an modality because its unique ability to detect a disturbed molecular pathway through measurements glucose uptake. However, FDG-PET limitations that restrict...