A5047945584- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Atomic and Subatomic Physics Research
- Enzyme Structure and Function
- Solid-state spectroscopy and crystallography
- Prostate Cancer Treatment and Research
- Medical Imaging Techniques and Applications
- NMR spectroscopy and applications
- Lanthanide and Transition Metal Complexes
- Boron Compounds in Chemistry
- Hormonal and reproductive studies
- Liver Disease Diagnosis and Treatment
- Protein Structure and Dynamics
- Nuclear Physics and Applications
- Prostate Cancer Diagnosis and Treatment
- Radiopharmaceutical Chemistry and Applications
- Neuroendocrine Tumor Research Advances
- MRI in cancer diagnosis
- Protease and Inhibitor Mechanisms
- Ultrasound and Hyperthermia Applications
- Mechanisms of cancer metastasis
- Immunotherapy and Immune Responses
- Amino Acid Enzymes and Metabolism
- Renal cell carcinoma treatment
University of California, San Francisco
2016-2025
Neurological Surgery
2010-2025
University of California System
2016-2025
Laboratoire d’Imagerie Biomédicale
2015
New York University
2011
Graduate Theological Union
2010
Advanced Imaging Research (United States)
2009
Lawrence Livermore National Laboratory
2007
Lawrence Berkeley National Laboratory
2007
UCSF Helen Diller Family Comprehensive Cancer Center
2001-2005
Metabolic imaging with hyperpolarized pyruvate was used to safely and noninvasively visualize prostate tumors in patients.
An extraordinary new technique using hyperpolarized (13)C-labeled pyruvate and taking advantage of increased glycolysis in cancer has the potential to improve way magnetic resonance imaging is used for detection characterization prostate cancer. The aim this study was quantify, first time, differences [1-(13)C] its metabolic products between various histologic grades transgenic adenocarcinoma mouse (TRAMP) model. Fast spectroscopic techniques were image lactate, alanine, total carbon (THC =...
Abstract The transgenic adenocarcinoma of mouse prostate (TRAMP) is a well‐studied murine model cancer with histopathology and disease progression that mimic the human disease. To investigate differences in cellular bioenergetics between normal epithelial cells tumor cells, vivo MR spectroscopic (MRS) studies non‐proton nuclei, such as 13 C, TRAMP would be extremely useful. recent development method for retaining dynamic nuclear polarization (DNP) solution permits high signal‐to‐noise ratio...
We present for the first time dynamic spectra and spectroscopic images acquired in normal rats at 3T following injection of (13)C-1-pyruvate that was hyperpolarized by nuclear polarization (DNP) method. Spectroscopic sampling optimized signal-to-noise ratio (SNR) spectral resolution its metabolic products (13)C-1-alanine, (13)C-1-lactate, (13)C-bicarbonate. Dynamic were collected with a temporal 3 s from 90-mm axial slab using dual (1)H-(13)C quadrature birdcage coil to observe combined...
In order to compare in vivo metabolism between malignant gliomas and normal brain, 13C magnetic resonance (MR) spectroscopic imaging data were acquired from rats with human glioblastoma xenografts (U-251 MG U-87 MG) rats, following injection of hyperpolarized [1-13C]-pyruvate. The median signal-to-noise ratio (SNR) lactate, pyruvate, total observed carbon-13 resonances, as well their relative ratios, calculated voxels containing Gadolinium-enhanced tissue T1 postcontrast images for tumors...
Reduction and oxidation (redox) chemistry is involved in both normal abnormal cellular function, processes as diverse circadian rhythms neurotransmission. Intracellular redox maintained by coupled reactions involving NADPH, glutathione (GSH), vitamin C, well their corresponding oxidized counterparts. In addition to functioning enzyme cofactors, these reducing agents have a critical role dealing with reactive oxygen species (ROS), the toxic products of oxidative metabolism seen culprits...
Purpose Hyperpolarized carbon‐13 ( 13 C) metabolic imaging is a noninvasive modality for evaluating real‐time metabolism. The purpose of this study was to develop and implement experimental strategies using [1‐ C]pyruvate probe in vivo metabolism patients with brain tumors other neurological diseases. Methods C radiofrequency coils pulse sequences were tested phantom performed 3 Tesla whole‐body scanner. Samples polarized SPINlab system. Dynamic data acquired from 8 previously diagnosed...
Abstract High polarization of nuclear spins in liquid state through hyperpolarized technology utilizing dynamic has enabled the direct monitoring 13 C metabolites vivo at a high signal‐to‐noise ratio. Acquisition time limitations due to T 1 decay signal require accelerated imaging methods, such as compressed sensing, for optimal speed and spatial coverage. In this paper, design testing new echo‐planar three‐dimensional magnetic resonance spectroscopic (MRSI) sensing sequence is presented....
Abstract Purpose: To demonstrate dynamic imaging of a diffusible perfusion tracer, hyperpolarized [ 13 C]urea, for regional measurement blood flow in preclinical cancer models. Materials and Methods: A pulse sequence using balanced steady state free precession (bSSFP) was developed, with progressively increasing flip angles efficient sampling the magnetization. This allowed temporal volumetric C]urea signal. Regional signal dynamics were quantified kidneys liver, estimates relative flows...
MRI using hyperpolarized (HP) carbon‐13 pyruvate is being investigated in clinical trials to provide non‐invasive measurements of metabolism for cancer and cardiac imaging. In this project, we applied HP [1‐ 13 C]pyruvate dynamic prostate measure the conversion from lactate, which expected increase aggressive cancers. The goal work was develop test analysis methods improved quantification metabolic conversion. work, compared specialized kinetic modeling estimate pyruvate‐to‐lactate rate, k P...
Abstract Background Hyperpolarized (HP) 13 C-pyruvate MRI is a stable-isotope molecular imaging modality that provides real-time assessment of the rate metabolism through glycolytic pathways in human prostate cancer. Heretofore this has been successfully utilized cancer only localized disease. This pilot clinical study investigated feasibility and performance HP MR metabolic patients with metastases to bone and/or viscera. Methods Six who had metastatic castration-resistant were recruited....
Abstract MRI with hyperpolarized (HP) 13 C agents, also known as HP MRI, can measure processes such localized metabolism that is altered in numerous cancers, liver, heart, kidney diseases, and more. It has been translated into human studies during the past 10 years, recent rapid growth largely based on increasing availability of agent preparation methods suitable for use humans. This paper aims to capture current successful practices [1‐ C]pyruvate—by far most commonly used agent, which sits...
PURPOSE: PC-SPES is an herbal supplement for which there are anecdotal reports of anti–prostate cancer activity. This phase II study was undertaken to assess the efficacy and toxicity in prostate patients. PATIENTS AND METHODS: Thirty-three patients with androgen-dependent (ADPCa) 37 androgen-independent (AIPCa) were treated at a dose nine capsules daily. Clinical outcome assessed serial serum prostate-specific androgen (PSA) level measurement imaging studies. RESULTS: One hundred percent...
Abstract Formulation, polarization, and dissolution conditions were developed to obtain a stable hyperpolarized solution of [1‐ 13 C]‐ethyl pyruvate. A maximum tolerated concentration injection rate determined, C spectroscopic imaging was used compare the uptake pyruvate relative C]‐pyruvate into anesthetized rat brain. Hyperpolarized metabolic in normal brain is demonstrated quantified this feasibility range‐finding study. Magn Reson Med 63:1137–1143, 2010. © 2010 Wiley‐Liss, Inc.
To evaluate the herbal combination, PC-SPES, and diethylstilbestrol (DES) in patients with androgen independent prostate cancer (AIPC).A randomized phase II study was conducted cross-over design. Patients were randomly assigned to receive either three PC-SPES capsules orally times a day or DES 3 mg once day. Prophylactic warfarin administered. At clinical prostate-specific antigen progression, received other therapy. The closed prematurely after withdrawn from market. Chemical analyses...
Abstract One of the challenges optimizing signal‐to‐noise ratio (SNR) and image quality in 13 C metabolic imaging using hyperpolarized C‐pyruvate is associated with different MR signal time‐courses for pyruvate its products, lactate alanine. The impact acquisition time window, variation flip angles, order phase encoding on SNR were evaluated mathematical simulations rat experiments, based multishot fast chemical shift (CSI) three‐dimensional echo‐planar spectroscopic (3DEPSI) sequences....
Hyperpolarized 13C labeled molecular probes have been used to investigate metabolic pathways of interest as well facilitate in vivo spectroscopic imaging by taking advantage the dramatic signal enhancement provided DNP. Due limited lifetime hyperpolarized nucleus, with decay dependent on T1 relaxation, carboxylate carbons primary targets for development probes. The use these carbon nuclei makes it difficult upstream glycolytic processes, which related both cancer metabolism other...
Abstract Purpose: To demonstrate the feasibility of using DNP hyperpolarized [1‐ 13 C]‐pyruvate to measure early response temozolomide (TMZ) therapy an orthotopic human glioblastoma xenograft model. Materials and Methods: Twenty athymic rats with intracranial implantation cells were divided into two groups: one group received oral administration 100 mg/kg TMZ ( n = 10) control vehicle only 10). C 3D magnetic resonance spectroscopic imaging (MRSI) data acquired following injection 2.5 mL (100...