A5071133382- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Animal Behavior and Reproduction
- Insect and Arachnid Ecology and Behavior
- Sleep and Wakefulness Research
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Adipose Tissue and Metabolism
- Neural dynamics and brain function
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Earth Systems and Cosmic Evolution
- Educational Methods and Impacts
- Olfactory and Sensory Function Studies
- Microbial infections and disease research
- Cellular Mechanics and Interactions
- Invertebrate Immune Response Mechanisms
- Paranormal Experiences and Beliefs
- Plant Molecular Biology Research
- Axon Guidance and Neuronal Signaling
- Physiological and biochemical adaptations
- Protein Hydrolysis and Bioactive Peptides
- Photoreceptor and optogenetics research
Harvard University
2015-2023
Rockefeller University
2012
Rutgers, The State University of New Jersey
2002-2008
Howard Hughes Medical Institute
2008
In bacteria, promoter recognition depends on the RNA polymerase σ subunit, which combines with catalytically proficient core to form holoenzyme. The major class of bacterial promoters is defined by two conserved elements (the –10 and –35 elements, are 10 35 nucleotides upstream initiation point, respectively) that contacted in We show this “flexible flap” domain β subunit. flap interacts region 4 triggers a conformational change moves into correct position for interaction element. Because...
Sleeping Around the Clock Fruit flies do not sleep all night like humans, but they manage to spend about half of their day in bouts inactivity that have similarity other species. Rogulja and Young (p. 1617 ) searched for genes neurons whose products regulated sleep. Depletion protein regulator cyclin A1 (Rca1) caused be slow go get less total a because episodes were shorter. The mechanism by which Rca1 influences is yet clear. It appears disrupt circadian rhythms. However, express fly brain...
Newborns and hatchlings can perform incredibly sophisticated behaviors, but many animals abstain from sexual activity at the beginning of life. Hormonal changes have long been known to drive both physical behavioral during adolescence, leading largely untested assumption that sexuality emerges organizational neuronal circuitry. We show transition in male Drosophila is controlled by hormonal changes, this regulation functional rather than structural. In very young males, a broadly acting...
Abstract Recent studies reported that METTL4 regulates DNA 6mA in vivo and therefore is a candidate m6A methyltransfease. However, the enzymatic activity of vitro has not been demonstrated part due to difficulties obtaining well-folded proteins. Here we show mettl4 major methyltransfase responsible for methylation U2 snRNA both fly, identify adenosine at 29th position as site methylation. This study answered long-standing question regarding METTL4, thus paved way further investigating...
Daily physical remodeling of neurons changes the internal sense daytime and nighttime.
SUMMARY Newborns and hatchlings of many species perform incredibly sophisticated behaviors, but all vertebrates invertebrates selectively abstain from sexual activity at the beginning life. Hormonal changes have long been associated with adolescence, it is not clear how these circulating factors create a new motivation drive its behaviors. We show that transition to sexuality in male Drosophila controlled by juvenile hormone, which spikes eclosion declines over days as propensity for...
Since sensory information is always present in the environment, animals need to internally regulate their responsiveness fit context. During sleep, threshold for arousal increased so that only stimuli of sufficient magnitude can cross it. The mechanisms make arousability flexible are largely mysterious, but they must integrate with about physiology. We discovered a gut-to-brain signaling pathway uses ingested nutrients control from without affecting sleep duration. Protein ingestion causes...
We used yeast two-hybrid and in vitroco-immobilization assays to study the interaction between theEscherichia coli RNA polymerase (RNAP) α β subunits during formation of α2β, a physiological RNAP assembly intermediate. show that 430-amino acid-long fragment containing conserved segments F, G, H, short part segment I forms minimal domain capable specific with α. The α-interacting is held together by protein-protein interactions F I. Residues catalytically important H directly participate...
Abstract Recent studies reported that METTL4 regulates DNA 6mA in vivo and therefore is a candidate m6A methyltransfease. However, the enzymatic activity of vitro has not been demonstrated part due to difficulties obtaining well-folded proteins. Here we show mettl4 major methyltransfase responsible for methylation U2 snRNA both fly, identify adenosine at 29th position as site methylation. This study answered long-standing question regarding METTL4, thus paved way further investigating...