A5101729690- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
- Pneumonia and Respiratory Infections
- Lipid Membrane Structure and Behavior
- Microbial bioremediation and biosurfactants
- Mine drainage and remediation techniques
- Groundwater and Watershed Analysis
- Computational Drug Discovery Methods
- Water resources management and optimization
- Infections and bacterial resistance
- Metal Extraction and Bioleaching
- Water Quality and Pollution Assessment
- Effects and risks of endocrine disrupting chemicals
- Metabolism and Genetic Disorders
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Toxic Organic Pollutants Impact
- Microbial Community Ecology and Physiology
Wockhardt (India)
2020-2023
National University of Singapore
2017-2020
Indian Institute of Technology Bombay
2007-2011
Biogenesis of the outer membrane (OM) in Gram-negative bacteria, which is essential for viability, requires coordinated transport and assembly proteins lipids, including lipopolysaccharides (LPS) phospholipids (PLs), into membrane. While pathways LPS OM protein are well-studied, how PLs transported to from not clear. Mechanisms that ensure stability homeostasis also unknown. The trans-envelope Tol-Pal complex, whose physiological role has remained elusive, important stability. Here, we...
Compared to other genera of Gram-negative pathogens,
Abstract Background Recent reports reveal the emergence of Escherichia coli isolates harbouring a novel resistance mechanism based on four-amino-acid inserts in PBP3. These organisms concomitantly expressed ESBLs or/and serine-/metallo-carbapenemases and were phenotypically detected by elevated aztreonam/avibactam MICs. Objectives The vitro activities investigational antibiotic cefepime/zidebactam approved antibiotics (ceftazidime/avibactam, ceftolozane/tazobactam, imipenem/relebactam...
Pseudomonas putida CSV86 shows preferential utilization of aromatic compounds over glucose. Protein analysis and [¹⁴C]glucose-binding studies the outer membrane fraction cells grown on different carbon sources revealed a 40 kDa protein that was transcriptionally induced by glucose repressed aromatics succinate. Based 2D gel electrophoresis liquid chromatography-tandem mass spectrometry analysis, closely resembled porin B P. KT2440 carbohydrate-selective OprB various strains. The purified...
Pseudomonas putida CSV86 utilizes aromatic compounds in preference to glucose and coutilizes aromatics organic acids. Protein analysis of cells grown on different carbon sources, either alone or combination, revealed that a 43-kDa periplasmic-space protein was induced by repressed succinate. Two-dimensional gel electrophoresis liquid chromatography-tandem mass spectrometry identified this as closely resembling the sugar ABC transporter KT2440. A partially purified showed binding activity...
Metabolic pathways for phenylaceticacid (PA) and 4-hydroxyphenylacetic acid (4-HPA) from Pseudomonas putida CSV86 were elucidated. PA grown strain cells showed higher oxygen-uptake on as compared to hydroxyphenylacetic acids. Detection of phenylacetyl-CoA ligase absence hydroxylase (4HPAH) 3,4-dihydroxyphenylacetic acid-dioxygenase (3,4-DHPADO) activity supports this observation. 4-HPA (3,4-DHPA) but significantly lower respiration rates 2,5-dihydroxyphenylacetic PA. 4HPAH 3,4-DHPADO...
The outer membrane (OM) is an essential component of the Gram-negative bacterial envelope that protects cells against external threats. To maintain a functional OM, require distinct mechanisms to ensure balance proteins and lipids in membrane. Mutations OM biogenesis and/or homeostasis pathways often result permeability defects, but how molecular changes affect barrier function unclear. Here, we seek potential mechanism(s) can alleviate defects Escherichia coli lacking Tol-Pal complex, which...
Water is a unique substance. Its liquid face predominates on the Earth. of Kolar reservoir was investigated to assess its water quality status. Study reveals that physico – chemical characteristics largely varied through change season, degree anthropogenic activities in and around ,the composition runoff catchment area. samples were collected during winter summer analysed for various parameters with special reference heavy metals e.g. Iron Manganese. A perusual analysis revealed Manganese...
Summary Biogenesis of the outer membrane (OM) in Gram-negative bacteria, which is essential for viability, requires coordinated transport and assembly proteins lipids, including lipopolysaccharides (LPS) phospholipids (PLs), into membrane. While pathways LPS OM protein are well-studied, how PLs transported to from not clear. Mechanisms that ensure stability homeostasis also unknown. The trans-envelope Tol-Pal complex, whose physiological role has remained elusive, important stability. Here,...
Summary The outer membrane (OM) is an essential component of the Gram-negative bacterial envelope that protects cells against external threats. To maintain a functional OM, require distinct mechanisms to ensure balance proteins and lipids in membrane. Mutations OM biogenesis and/or homeostasis pathways often result permeability defects, but how molecular changes affect barrier function unclear. Here, we seek potential mechanism(s) can alleviate defects Escherichia coli lacking Tol-Pal...