How chromosomes are folded, spatially organized and regulated in three dimensions inside the cell nucleus among longest standing questions biology. Genome-wide chromosome conformation capture (Hi-C) technique allowed identifying characterizing spatial chromatin compartments several mammalian species. Here, we present first genome-wide analysis of interactions chicken embryonic fibroblasts (CEF) adult erythrocytes. We showed that genome CEF is partitioned into topologically associated domains...

Wolbachia bacteria are common endosymbionts of many arthropods found in gonads and various somatic tissues. They manipulate host reproduction to enhance their transmission confer complex effects on fitness-related traits. Some these can serve increase the survival efficiency population. The Wolbachia-Drosophila melanogaster system represents a powerful model study evolutionary dynamics host-microbe interactions infections. Over past decades, there has been replacement ancestral wMelCS...

Wolbachia are maternally transmitted intracellular bacteria that not only restricted to the reproductive organs but also found in various somatic tissues of their native hosts. The abundance endosymbiont soma, usually a dead end for vertically bacteria, causes multitude effects on life history traits hosts, which still well understood. Thus, deciphering host-symbiont interactions cellular level throughout host's cycle is great importance understand homeostatic nature, persistence, and...

Endothelial and smooth muscle cells are considered promising resources for regenerative medicine cell replacement therapy. It has been shown that both types of heterogeneous depending on the type vessels organs in which they located. Therefore, isolation endothelial from tissues relevant to area research is necessary adequate study specific pathologies. However, sources specialized human limited, search new still relevant. The main goal our demonstrate functional can be obtained an available...

Objective. To identify cell phenotypes in vertebral body growth plates from patients with idiopathic scoliosis. Material and Methods . Cells were isolated both on convex concave sides of the deformity operated for cultured identified by methods common morphology, neuromorphology, electron microscopy, immunohistochemistry, PCR analysis. Results Cultured cells obtained side as chondroblasts. described neuro- glioblasts. formed synapses, contained neurofilaments, expressed neural glial...

Microbial symbionts are universal entities of all living organisms that can significantly affect host fitness traits in manifold ways but, even more fascinating, also their behaviour. Although better known from parasitic symbionts, we currently lack any cases where 'neurotrophic' have co-evolved mutualistic behavioural interactions which both partners profit. By theory, most associations originated ancestral ones during long-term co-evolution towards a cost–benefit equilibrium. To manipulate...

Abstract Temperature plays a fundamental role in the fitness of all organisms. In particular, it strongly affects metabolism and reproduction ectotherms that have limited physiological capabilities to regulate their body temperature. The influence temperature variation on physiology behaviour is well studied but we still know little about symbiotic interactions thermal preference ( T p ) host. A growing number studies focusing Wolbachia ‐ Drosophila host‐symbiont system found can laboratory...

The pathogenic Wolbachia strain wMelPop rapidly over-replicates in the brain, muscles, and retina of Drosophila melanogaster, causing severe tissue degeneration premature death host. unique features this endosymbiont make it an excellent tool to be used for biological control insects, pests, vectors human diseases. To follow dynamics bacterial morphology titer nerve cells we transmission electron microscopy 3-d-old female brains. neurons glial from central brain fly had different titers...

Abstract Wolbachia is one of the most common bacterial endosymbionts, which frequently found in numerous arthropods and nematode taxa. infections can have a strong influence on evolutionary dynamics their hosts since these bacteria are reproductive manipulators that affect fitness life history host species for own benefit. Host–symbiont interactions with perhaps best studied model organism Drosophila melanogaster, naturally infected at least 5 different variants among wMel wMelCS frequent...

Biological activity of antisense oligonucleotides (asON), especially those with a neutral backbone, is often attenuated by poor cellular accumulation. In the present proof-of-concept study, we propose novel delivery system for asONs which implies modified so-called transport (tON), are oligodeoxyribonucleotides complementary to asON conjugated hydrophobic dodecyl moieties. Two types tONs, bearing at 5′-end up three residues attached through non-nucleotide inserts (TD series) or anchored...

Temperature fluctuations are challenging for ectotherms which not able to regulate body temperature by physiological means and thus have adjust their thermal environment via behavior. However, little is yet known about whether microbial symbionts influence preference (T

INT6/eIF3e is a highly conserved component of the translation initiation complex that interacts with both 26S proteasome and COP9 signalosome, two complexes implicated in ubiquitin-mediated protein degradation. The INT6 gene was originally identified as insertion site mouse mammary tumor virus (MMTV), later shown to be involved human tumorigenesis. Here we show depletion Drosophila orthologue (Int6) results short mitotic spindles deformed centromeres kinetochores low intra-kinetochore...

S2 cells are one of the most widely used Drosophila melanogaster cell lines. A series studies has shown that they particularly suitable for RNAi-based screens aimed at dissection cellular pathways, including those controlling shape and motility, metabolism, host-pathogen interactions. In addition, RNAi in been successfully to identify many new mitotic genes conserved higher eukaryotes, analysis several aspects process. However, no detailed complete description mitosis ultrastructural level...

Idiopathic scoliosis is one of the most common disabling pathologies children and adolescents.Etiology pathogenesis idiopathic remain unknown.To study etiology this disease we identified cells' phenotypes in vertebral body growth plates patients with scoliosis.Materials methods: The cells were isolated from convex concave sides deformity harvested intraoperatively 50 scoliosis.Cells cultured by methods morphology, neuromorphology, electron microscopy, immunohistochemistry PCR...

Tsetse flies cause major health and economic problems as they transmit trypanosomes causing sleeping sickness in humans (Human African Trypanosomosis, HAT) nagana animals (African Animal AAT). A solution to control the spread of these their associated diseases is implementation Sterile Insect Technique (SIT). For successful application SIT, it important establish maintain healthy insect colonies produce with competitive fitness. However, mass production tsetse threatened by covert virus...

Graphical abstractAbstractThe Drosophila S2 tissue culture cells are a widely used system for studies on mitosis. particularly sensitive to gene silencing by RNA interference (RNAi), allowing targeted inactivation of mitotic genes. also well suited high-resolution light microscopy analysis mitosis in fixed cells, and can be easily immunostained detect components. In addition, amenable transformation with plasmid encoding fluorescently tagged proteins, vivo their behavior throughout cell...

Abstract The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist two major chromosomal races with different sex chromosome systems, XO/XX XY/XX. Molecular phylogeographic analysis subraces confirms genetic divergence in P. . Here we first report that infected Wolbachia consisting three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation...

Abstract Wolbachia is one of the most common bacterial endosymbionts, which frequently found in numerous arthropods and nematode taxa. infections can have a strong influence on evolutionary dynamics their hosts since these bacteria are reproductive manipulators that affect fitness life history host species for own benefit. Host-symbiont interactions with perhaps best studied model organism Drosophila melanogaster , naturally infected five different types among wMel wMelCS frequent ones....

A new protocol for production of functional endothelial and mural cells from human cardiac explants has been developed. The are characterized by the presence mature markers: CD 31, VE-cadherin VEGFR2. Functionally they could take up ac-LDL, form tube-like structure in matrigel, contains cytoplasmic microvesicles Weibel-Palade bodies, cytoplasm – metabolized acetylated low-density lipoprotein, capillary-like structures Matrigel produces extracellular matrix. smooth muscle expressed a specific...