A5072984677- Laser Design and Applications
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Lipoproteins and Cardiovascular Health
- Laser-Matter Interactions and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Organophosphorus compounds synthesis
- Chromosomal and Genetic Variations
- Advanced Fiber Laser Technologies
- Photochemistry and Electron Transfer Studies
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Solid State Laser Technologies
- Phosphorus compounds and reactions
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Luminescence Properties of Advanced Materials
- Coronary Interventions and Diagnostics
- Human Health and Disease
- Spectroscopy and Laser Applications
Institute of Cytology and Genetics
2010-2024
Meshalkin National Medical Research Center
2013-2022
Institute of Chemical Biology and Fundamental Medicine
2013-2022
Siberian Branch of the Russian Academy of Sciences
2013-2022
Ministry of Health of the Russian Federation
2013-2022
Moscow State Pedagogical University
2002-2003
National Academy of Sciences of Belarus
1970-1992
Glucocorticoids (GC) are included in the pathogenetic therapy for severe complicated forms of neuroinfections due to their anti-inflammatory, anti-edematous, and anti-shock effects. GCs have a suppressive effect on hypothalamic-pituitary-adrenal system, mainly as result long-term treatment courses. At same time, secretion cortisol by adrenal glands decreases. The article shows short courses glucocorticoid serum level at different periods bacterial meningitis. It was found that introduction...
Familial hypercholesterolaemia is a common monogenic disorder characterized by high plasma cholesterol levels leading to chronic cardiovascular disease with risk and often early manifestation due atherosclerotic lesions of the blood vessels. The in familial are mainly caused pathogenic variants low-density lipoprotein receptor ( LDLR ) gene, which plays an important role metabolism. Normally, cholesterol-laden lipoproteins bind on surface liver cells be removed from bloodstream...
X inactivation, the transcriptional silencing of one two chromosomes in female mammals, achieves dosage compensation X-linked genes relative to XY males. In eutherian mammals inactivation is regulated by X-inactive specific transcript (Xist), a cis-acting non-coding RNA that triggers chromosome from which it transcribed. Marsupial also undergo but mechanism relatively poorly understood. We set out analyse Monodelphis domestica and Didelphis virginiana, focusing on characterizing interval...
Endothelial and smooth muscle cells are considered promising resources for regenerative medicine cell replacement therapy. It has been shown that both types of heterogeneous depending on the type vessels organs in which they located. Therefore, isolation endothelial from tissues relevant to area research is necessary adequate study specific pathologies. However, sources specialized human limited, search new still relevant. The main goal our demonstrate functional can be obtained an available...
Rat pluripotent stem cells, embryonic cells (ESCs), and induced (iPSCs) as mouse human ones have a great potential for studying mammalian early development, disease modeling, evaluation of regenerative medicine approaches. However, data on pluripotency realization self-renewal maintenance in rat are still very limited, differentiation protocols ESCs (rESCs) iPSCs to study development obtain specific cell types biomedical applications poorly developed. In this study, the RNA-Seq technique was...
The LDLR locus has clinical significance for lipid metabolism, Mendelian familial hypercholesterolemia (FH), and common metabolism-related diseases (coronary artery disease Alzheimer’s disease), but its intronic structural variants are underinvestigated. aim of this study was to design validate a method nearly complete sequencing the gene using long-read Oxford Nanopore technology (ONT). Five PCR amplicons from three patients with compound heterozygous FH were analyzed. We used standard...
X chromosome inactivation is a complex process that occurs in marsupial and eutherian mammals. The thought to have arisen during the differentiation of mammalian sex chromosomes achieve an equal dosage genes males females. differences processes mammals are considered, hypotheses on its origin evolution discussed this review.
Defects in the low-density lipoprotein receptor (LDLR) are associated with familial hypercholesterolemia (FH), manifested by atherosclerosis and cardiovascular disease. LDLR deficiency hepatocytes leads to elevated blood cholesterol levels, which damage vascular cells, especially endothelial through oxidative stress inflammation. However, distinctions between cells from individuals normal defective not yet fully understood. In this study, we obtained examined derivatives of induced...
In our previous study, we showed that discarded cardiac tissue from the right atrial appendage and ventricular myocardium is an available source of functional endothelial smooth muscle cells for regenerative medicine engineering. aimed to find out what benefits are given by vascular explants used seeding on patches engrafted repair defects in vivo. Additionally, make application these safer tested vitro approach arrested mitotic division avoid potential tumorigenic effect dividing cells. A...
Familial hypercholesterolemia (FH) is a monogenic disease, leading to atherosclerosis due high level of low-density lipoprotein cholesterol. Most cases the disease are based on pathological variants in LDLR gene. Hepatocyte-like and endothelial cells derived from individual iPSCs good model for developing new approaches therapy. We obtained an iPSC line peripheral blood mononuclear patient with compound heterozygous p.Ser177Leu/p.Cys352Arg mutation using non-integrating vectors. The...
For the first time, two types of hybrid cells with embryonic stem (ES) cell-like and fibroblast-like phenotypes were produced through fusion mouse ES fibroblasts. Transcriptome analysis 2,848 genes differentially expressed in parental demonstrated that 34-43% these are cells, consistent their phenotypes; 25-29% display intermediate levels expression, 12-16% maintained expression at cell level, inconsistent phenotype cell. Approximately 20% analyzed displayed unexpected patterns differ from...
Familial hypercholesterolemia (FH) is an autosomal dominant disorder increasing premature cardiovascular diseases risk due to atherosclerosis. Pathogenic mutations in the LDLR gene cause most FH cases. Available treatments are effective not for all mutations. Testing drugs on cell models help develop new efficient treatments. We obtained iPSC line from peripheral blood mononuclear cells of patient with heterozygous p.Trp443Arg mutation. The iPSCs confirmed patient-specific express...
Defects in low-density lipoprotein receptor (LDLR) are associated with familial hypercholesterolemia (FH), manifested by atherosclerosis and cardiovascular disease. Defective LDLR hepatocytes leads to increased blood cholesterol level that damage vascular cells, especially endothelial through oxidative stress inflammation. However, the distinctions between cells from individuals normal defective not yet fully comprehended. In this study, we obtained examined derivatives of induced...
The development of cellular models for familial hypercholesterolemia (FH) is an important direction creating new approaches to atherosclerosis treatment. Pathogenic mutations in the LDLR gene are main FH source. We generated iPSC line from peripheral blood mononuclear cells patient with compound heterozygous c.1246C > T/c.940 + 3_940 6del mutation. resulting confirmed patient-specific maintains a normal karyotype and typical undifferentiated state, including morphology, pluripotent...
Objective. The study focused on the production and evaluation of small-diameter vascular grafts (less than 6 mm) by using an electrospinning approach. Methods. protocols (VG) to be produced from solutions synthetic polymers their blends with gelatin including those a semipermeable inner layer were developed. comparative in vitro vivo was carried out. resistance VG displacement, suture retention, layers snagging compatibility native artery walls studied. Also assessed time hemostasis when...
<p>Характерный маркер кардиальных стволовых клеток – тиразинкиназный рецептор c-kit. Клетки с фенотипом c-kit+ легко выделяются и культивируются in vitro. Однако истинным кардиомиогенным потенциалом обладают только кардиальные стволовые клетки, полученные из сердца постнатальных животных. Исследователи предполагают две популяции в сердце различного происхождения эмбриогенезе. c-kit+, способные дифференцироваться кардиомиоциты, происходят первичного кардиального поля исчезают вскоре...