A5081016203- Parasitic Infections and Diagnostics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Poxvirus research and outbreaks
- Rabies epidemiology and control
- RNA modifications and cancer
- Veterinary medicine and infectious diseases
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
Weizmann Institute of Science
2024-2025
Indian Institute of Science Bangalore
2019-2022
Transfer RNA (tRNA) modifications are crucial for protein synthesis, but their position-specific physiological roles remain poorly understood. Here, we investigate the impact of N 4 -acetylcytidine (ac C), a highly conserved tRNA modification catalyzed by essential acetyltransferase Nat10. By targeting Thumpd1, nonessential adapter required Nat10-catalyzed acetylation, determine that loss acetylation leads to reduced levels Leu , increased ribosome stalling, and activation eIF2α...
We have previously shown that the Heat Shock Protein 90 (Hsp90) gene in G. lamblia is expressed from two ORFs localized 777 kb apart. The pre-mRNAs transcribed these are stitched by a trans-splicing mechanism. Here, we provide mechanistic details of this process reconstituting reaction using vitro synthesized pre-mRNA substrates. Using RT-PCR, northern blot and nanostring technology, demonstrate capability to self-splice absence nuclear proteins. Inhibition ssDNA oligo corresponding...
ABSTRACT Transfer RNA (tRNA) modifications are crucial for protein synthesis, but their position-specific physiological roles remain poorly understood. Here we investigate the impact of N4-acetylcytidine (ac 4 C), a highly conserved tRNA modification, using Thumpd1 knockout mouse model. We find that loss Thumpd1-dependent acetylation leads to reduced levels Leu , increased ribosome stalling, and activation eIF2α phosphorylation. mice exhibit growth defects sterility. Remarkably, concurrent...
We report five canine rabies virus genome sequences from India that were obtained brain samples using Oxford Nanopore Technologies sequencing. The will facilitate understanding of the evolution and transmission rabies.
Hsp90 gene of G . lamblia has a split nature comprising two ORFs separated by 777 kb on chromosome 5. The the 5 undergo transcription to generate independent pre-mRNAs that join unique trans -splicing reaction remains partially understood. canonical cis-acting nucleotide elements such as 5’SS-GU, 3’SS-AG, polypyrimidine tract and branch point adenine are present in therefore must be assisted spliceosomes vivo Using an approach RNA-protein pull down, we show RNA helicase selectively interacts...