A5100627907- Hearing, Cochlea, Tinnitus, Genetics
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Mitochondrial Function and Pathology
- Connexins and lens biology
- Vestibular and auditory disorders
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Congenital heart defects research
- RNA Research and Splicing
- Ion channel regulation and function
- Ear Surgery and Otitis Media
- Genomic variations and chromosomal abnormalities
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Molecular Biology Techniques and Applications
- Multiple Myeloma Research and Treatments
- Nutrition, Genetics, and Disease
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
Chinese PLA General Hospital
2007-2025
Heilongjiang Bayi Agricultural University
2013-2025
National Clinical Research Center for Digestive Diseases
2020-2024
Nankai University
2024
National Clinical Research
2024
Jilin Agricultural University
2024
Sichuan Cancer Hospital
2021
University of Electronic Science and Technology of China
2021
Chinese People's Liberation Army
2007-2021
Ministry of Education of the People's Republic of China
2020-2021
The cancer stem cell hypothesis suggests that malignant growth depends on a subset of tumor cells with cell-like properties self-renewal. Because hedgehog (Hh) signaling regulates progenitor fate in normal development and homeostasis, aberrant pathway activation might be involved the maintenance such population cancer. Indeed, mutational Hh is associated medulloblastoma basal carcinoma; activity also critical for other tumors lacking mutations, although mechanism poorly understood. Here we...
Many DNA-hypermethylated cancer genes are occupied by the Polycomb (PcG) repressor complex in embryonic stem cells (ESCs). Their prevalence full spectrum of cancers, exact context chromatin involved, and their status adult cell renewal systems unknown. Using a genome-wide analysis, we demonstrate that ∼75% hypermethylated marked PcG bivalent both ESCs stem/progenitor cells. A large number these key developmental regulators, subset, which call “DNA hypermethylation module,” comprises portion...
Free range feeding pattern puts the chicken in a mixture of growth materials and enteric bacteria excreted by nature, while it is typically unique condition commercial caged hens production. Thus, gastrointestinal microflora two patterns could be various. However, remains poorly understood how affect development composition layer hens' intestinal microflora. In this study, effect on community gut was investigated using free form. Samples whole small intestines cecal digesta were collected...
Conclusion. This genetic epidemiological study demonstrated that 26.65% of the prelingual deafness in Northern Chinese patients can be detected at younger ages by testing three common hearing loss genes (GJB2, SLC26A4 and mtDNA A1555G), thus, early intervention measures could undertaken to help them language acquisition. Objectives. The GJB2, A1555G mutations are prevalent causes worldwide. Numerous studies have revealed forms frequencies largely dependent on ethnic or geographic origins....
Activation of the Hedgehog pathway drives FLT3-mutated leukemia, and dual inhibition effectively inhibits tumor growth.
Auditory neuropathy (AN) is a unique type of language developmental disorder, with no precise rate genetic contribution that has been deciphered in large cohort. In retrospective cohort 311 patients AN, pathogenic and likely variants 23 genes were identified 98 (31.5% patients), 14 mutated two or more patients. Among subgroups the prevalence was 54.4% 56.2% trios families, while 22.9% cases proband-only; 45.7% 25.6% infant non-infant group; 33.7% 0% bilateral unilateral AN cases. Most OTOF...
Mutations in mitochondrial DNA (mtDNA), particularly those the 12S rRNA gene, have been shown to be associated with sensorineural hearing loss. Recently, a systematic and extended mutation screening of gene has initiated large clinical population Otology Clinic at Chinese PLA General Hospital aim identifying mtDNA mutations Here we report molecular characterization patient auditory neuropathy. Sequence analysis this identified T-to-C transition position 1095 (T1095C) other nucleotide...
The effects of Enterococcus faecalis (E. faecalis) at a concentration 1.0 × 108 CFU/mL on growth performance, hepatic lipid metabolism, and mRNA expression related to intestinal morphology, flora were investigated in geese. A total 60 male geese, aged 30 days similar weight, randomly assigned 2 groups. Each group was divided into six replicates, with five geese per replicate. During the 45-day experiment, control received basal diet, while experimental provided same diet supplemented E....
The Hedgehog (Hh) signaling pathway is aberrantly activated in a wide variety of human cancers, and recent clinical studies have demonstrated that inhibitors are effective advanced basal cell carcinoma (BCC). majority these agents been designed to target SMOOTHENED (SMO), transmembrane regulator Hh signaling, but subsequent mutations SMO found generate drug resistance. In other oncogenic events bypass may activate canonical antagonists not significant activity several diseases. Therefore,...
Background: Auditory brainstem response (ABR) testing is an invasive electrophysiological auditory function test. Its waveforms and threshold can reflect functional changes in the centers are widely used clinic to diagnose dysfunction hearing. However, identifying its mainly dependent on manual recognition by experimental persons, which could be primarily influenced individual experiences. This also a heavy job clinical practice. Methods: In this work, human ABR was recorded. First,...
Mouse cytochrome P450 2A5 (CYP2A5) is upregulated in various liver diseases and a putative common feature for all of these conditions altered cellular redox status. Nuclear factor erythroid 2-like 2 (Nrf2) transcription that post-translationally regulated by oxidative stress controls the protective target genes. In present study, we have characterized regulation CYP2A5 Nrf2 evaluated gene expression, protein content activity anti-oxidant enzymes Nrf2+/+ Nrf2−/− mice model non-alcoholic fatty...
To report two DFNA5 pathogenic splice-site variations and a novel benign frameshift variation to further support the gain-of-function mechanism of related hearing impairment, targeted genes capture next generation sequencing were performed on selected members from Family 1007208, 1007081 sporadic case with sensorineural loss. Reverse transcriptase polymerase chain reaction was conducted proband 1007208 test how affects transcription in RNA level. A heterozygous c.991-3 C > found 1007208;...
Abstract Background: Aberrant HH pathway signaling has been implicated in human malignancies ranging from semi-malignant tumors of the skin to highly aggressive cancers brain, lung, pancreas, breast, prostate, and lymphohematopoietic lineages. Dysregulation this contributes uncontrolled proliferation, invasion, metastasis, evasion apoptosis, resistance chemotherapy. Results: BMS-833923 is a potent inhibitor SMO, GPCR-like 7-transmembrane receptor that critical regulator pathway. In vitro,...
To determine the genetic cause of non-syndromic autosomal dominant deafness segregating in a Chinese Auditory neuropathy (AN) family.AN is genetically related rare disease characterized by sensorineural hearing loss and retention hair cell function. Diaphanous Homolog 1 (DIAPH1) causative gene DFNA1. date, no evidence has been detected to reveal connection between DIAPH1 AN.Audiological imageological examinations, genome-wide linkage analysis, whole exome sequencing (WES) were carried out on...
Auditory neuropathy spectrum disorder (ANSD) is a hearing impairment caused by dysfunction of inner hair cells, ribbon synapses, spiral ganglion neurons and/or the auditory nerve itself. Approximately 1/7000 newborns have abnormal function, accounting for 10%-14% cases permanent loss in children. Although we previously identified AIFM1 c.1265 G > A variant to be associated with ANSD, mechanism which ANSD poorly understood. We generated induced pluripotent stem cells (iPSCs) from peripheral...