A5009011499- Antifungal resistance and susceptibility
- MicroRNA in disease regulation
- Enzyme Structure and Function
- Cancer-related molecular mechanisms research
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Mitochondrial Function and Pathology
- Advanced Nanomaterials in Catalysis
- Biochemical and Molecular Research
- Metal-Catalyzed Oxygenation Mechanisms
Hubei University
2025
Shandong First Medical University
2024
Second Affiliated Hospital of Xi'an Jiaotong University
2023
N–N bond formation plays a critical role in the synthesis of organic compounds and has broad applications producing dyes, pharmaceuticals, functional materials. However, is challenging due to nucleophilicity nitrogen. Here, we determined crystal structures heme-dependent enzyme, KtzT, which catalyzes cyclization l-N5-hydroxyornithine (l-N5-OH-Orn) yield l-piperazate (l-piz) by linking two intramolecular nitrogen atoms. The complex structure KtzTC197A with l-N5-OH-Orn reveals...
Echinocandin B (ECB) is a key precursor of the antifungal drug anidulafungin and its biosynthesis occurs via ani gene cluster in Aspergillus nidulans NRRL8112. Strain improvement for industrial ECB production has mainly relied on mutation breeding due to lack genetic tools. Here, CRISPR-base-editing tool was developed A. NRRL8112 simultaneous inactivation nkuA two marker genes, pryoA riboB, which enabled efficient manipulation. Then, in-vivo plasmid assembly harnessed expression screening,...