A5073796755- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Plant Virus Research Studies
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Postharvest Quality and Shelf Life Management
- Fungal Plant Pathogen Control
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Horticultural and Viticultural Research
- Nematode management and characterization studies
- Antimicrobial Peptides and Activities
- Plant Gene Expression Analysis
- Biochemical and Structural Characterization
- Banana Cultivation and Research
- Legume Nitrogen Fixing Symbiosis
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Resistance
- Toxin Mechanisms and Immunotoxins
- Plant responses to water stress
Appalachian Fruit Research Laboratory
2011-2023
United States Department of Agriculture
2008-2023
Agricultural Research Service
2011-2021
University of Nebraska at Kearney
2019-2020
Pennsylvania State University
2006
Cornell University
1991-2003
New York State Department of Agriculture and Markets
1990-2002
Plant (United States)
2000
High-throughput amplicon sequencing spanning conserved portions of microbial genomes (16s rRNA and ITS) was used in the present study to describe endophytic microbiota associated with three apple varieties, "Royal Gala," "Golden Delicious," "Honey Crisp," two rootstocks, M.9 M.M.111. The objectives were (1) determine if differs different rootstocks varieties (2) specific rootstock-scion combinations influence composition either component. Results indicated that Ascomycota (47.8%), Zygomycota...
The goal of this research was to improve scab resistance apple by transformation with genes encoding chitinolytic enzymes from the bio-control organism Trichoderma harzianum. endochitinase gene, as cDNA and genomic clones, transferred into cv. Marshall McIntosh Agrobacterium-transformation. A total 15 lines were identified transgenic NPTII enzyme-linked immunosorbent assay polymerase chain reaction confirmed Southern analysis. Substantial differences in activity detected among different...
The timing of cold acclimation and deacclimation, dormancy, budbreak play an integral role in the life cycle woody plants. molecular events that regulate these parameters have been subject much study, however, most studies investigated independently each other. Ectopic expression a peach CBF (PpCBF1) apple increases level both non-acclimated acclimated freezing tolerance relative to non-transformed control, also inhibits growth, induces early bud set leaf senescence, delays break spring....
Summary Plants evolved in association with a diverse community of microorganisms. The effect plant phylogeny and domestication on host–microbiome co‐evolutionary dynamics are poorly understood. Here we examined the lineage composition endophytic microbiome 11 Malus species, representing three major groups: domesticated apple ( M . domestica ), wild progenitors, species. its progenitors showed higher microbial diversity abundance than Heirloom modern cultivars harbored distinct composition,...
A pretreatment of the yeast, Candida oleophila, with 5 mM H(2)O(2) for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels oxidative stress (50 H(2)O(2)), high temperature (40 °C), and low pH (pH 4). Compared non-stress-adapted cells, stress-adapted cells exhibited better control apple fruit infections by Penicillium expansum Botrytis cinerea had initially higher growth rates in wounds. Suppression subtractive hybridization analysis was used identify genes expressed...
Breeding of fire blight resistant scions and rootstocks is a goal several international apple breeding programs, as options are limited for management this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) resistance has been reported on linkage group 3 Malus 'Robusta 5'. In study we identified markers derived from putative genes associated with QTL integrating further genetic mapping studies bioinformatics analysis...
The C-repeat binding factor (CBF) transcription is involved in responses to low temperature and water deficit many plant species. Overexpression of CBF genes leads enhanced freezing tolerance growth inhibition overexpression a peach (PpCBF1) gene transgenic line own-rooted apple (Malus×domestica) M.26 rootstock (T166) trees was previously reported have additional effects on the onset dormancy time spring budbreak. In current study, commercial cultivar 'Royal Gala' (RG) grafted onto either...
In response to environmental cues plants undergo changes in gene expression that result the up- or down-regulation of specific genes. To identify genes peach [ Prunus persica (L.) Batsch.] trees whose transcript levels are specifically affected by low temperature (LT) short day photoperiod (SD), we have created suppression subtractive hybridization (SSH) libraries from bark tissues sampled kept at 5 °C and 25 under (SD) exposed a night break (NB) interruption during dark period SD cycle...
Leaf, bark, xylem and root tissues were used to make nine cDNA libraries from non‐stressed (control) ‘Royal Gala’ apple trees, trees exposed either low temperature (5°C for 24 h) or water deficit (45% of saturated pot mass 2 weeks). Over 22 600 clones the subjected 5′ single‐pass sequencing, clustered annotated using blastx . The number clusters in ranged 170 1430. Regarding annotation sequences, analysis indicated that within 65–72% had a high similarity known function genes, 6–15% no...
CBF (C-repeat Binding Factor) transcription factors are part of the AP2/ERF (Apetala2-ethylene responsive factor) domain family DNA-binding proteins that recognize a C-repeat response cis-acting element regulates number cold-responsive genes (CBF regulon). Induction gene expression by low temperature in Arabidopsis has been shown to be gated circadian clock. In peach (Prunus persica L.), five arranged tandem on scaffold (linkage group) 5 genome. Since regulation more complex woody plants...
Fire blight (Erwinia amylovora) is a devastating bacterial disease in apple that results severe economic losses. Epidemics are becoming more common as susceptible cultivars and rootstocks being planted, control difficult antibiotic-resistant strains develop. Resistant germplasm currently utilized by breeding programs tend to have small fruit size poor flavor characteristics. Malus sieversii, progenitor species of domestic apple, notable for its relatively large, palatable some accessions...
Malus sieversii from Central Asia is a progenitor of the modern domesticated apple (Malus × domestica). Several accessions M. are highly resistant to postharvest pathogen Penicillium expansum. A previous study identified qM-Pe3.1 QTL on LG3 for resistance P. expansum in mapping population GMAL4593, developed using accession, -PI613981, and susceptible cultivar 'Royal Gala' (M. domestica), as parents. The goal present was characterize transcriptomic response PI613981 fruit wounding...