A5089576007- Superconducting Materials and Applications
- Particle accelerators and beam dynamics
- Particle Accelerators and Free-Electron Lasers
- Particle physics theoretical and experimental studies
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Cardiac Ischemia and Reperfusion
- Mesenchymal stem cell research
- Ion channel regulation and function
- Photochemistry and Electron Transfer Studies
- Epigenetics and DNA Methylation
- Crystallization and Solubility Studies
- Forensic Toxicology and Drug Analysis
- DNA and Nucleic Acid Chemistry
- X-ray Diffraction in Crystallography
- Cardiovascular Effects of Exercise
- Hormonal Regulation and Hypertension
- Computational Physics and Python Applications
- Cardiac Fibrosis and Remodeling
- Nitric Oxide and Endothelin Effects
- Particle Detector Development and Performance
- Prostate Cancer Treatment and Research
- Receptor Mechanisms and Signaling
- bioluminescence and chemiluminescence research
- Luminescence and Fluorescent Materials
Fermi National Accelerator Laboratory
2003-2024
Hudson Institute of Medical Research
2024
Carney Hospital
2014-2021
Tufts University
1995-2017
Boston University
2000-2015
St. Elizabeth's Medical Center
2006-2015
Swansea Bay University Health Board
2015
Morriston Hospital
2015
Morgan, Lewis & Bockius (United States)
2014
New York Proton Center
2014
Preeclampsia, a syndrome affecting 5% of pregnancies, causes substantial maternal and fetal morbidity mortality. The pathophysiology preeclampsia remains largely unknown. It has been hypothesized that placental ischemia is an early event, leading to production soluble factor or factors cause endothelial dysfunction, resulting in the clinical findings hypertension, proteinuria, edema. Here, we confirm fms-like tyrosine kinase 1 (sFlt1), antagonist VEGF growth (PlGF), upregulated preeclampsia,...
Massive loss of cardiac myocytes after myocardial infarction (MI) is a common cause heart failure. The present study was designed to investigate the improvement function in MI rats embryonic stem (ES) cell transplantation. induced by ligation left anterior descending coronary artery. Cultured ES cells used for transplantation were transfected with marker green fluorescent protein (GFP). Animals treated group received intramyocardial injection injured myocardium. Compared control injected an...
Abstract Many hypertrophic stimuli such as angiotensin II (Ang II) activate phospholipases through G protein–coupled receptors in cardiac myocytes. However, it is not known whether these also the tyrosine phosphorylation–dependent signaling pathway, which plays an essential role growth factor–induced mitogenic responses other cell types. Serine/threonine kinases mitogen-activated protein (MAP) and 90-kD S6 kinase (RSK) are activated response to many important downstream pathways of kinases....
Preeclampsia, a syndrome affecting 5% of pregnancies, causes substantial maternal and fetal morbidity mortality. The pathophysiology preeclampsia remains largely unknown. It has been hypothesized that placental ischemia is an early event, leading to production soluble factor or factors cause endothelial dysfunction, resulting in the clinical findings hypertension, proteinuria, edema. Here, we confirm fms-like tyrosine kinase 1 (sFlt1), antagonist VEGF growth (PlGF), upregulated preeclampsia,...
Vascular endothelial growth factor (VEGF) is an cell mitogen that thought to function by interacting with two high-affinity receptors, flk-1 and flt-1. In adult heart, angiogenesis can occur in a number of pathological conditions, including atherosclerosis, hypertrophy, infarction. To determine the role played VEGF, flk-1, flt-1 this process vivo, we studied expression its receptors rat infarct model. After acute myocardial infarction, observed initial rapid (1h) rise VEGF (275%), (375%),...
Recent evidence indicates that polyunsaturated long-chain fatty acids (PUFAs) prevent lethal ischemia-induced cardiac arrhythmias in animals and probably humans. To increase understanding of the mechanism(s) this phenomenon, effects PUFAs on Na+ currents were assessed by whole-cell patch-clamp technique cultured neonatal rat ventricular myocytes. Extracellular application free 5,8,11,14,17-eicosapentaenoic acid (EPA) produced a concentration-dependent suppression ventricular,...
<b><i>Background:</i></b> Mutations in <i>SCN1A</i>, the gene encoding α1 subunit of sodium channel, have been found severe myoclonic epilepsy infancy (SMEI) and generalized with febrile seizures plus (GEFS<sup>+</sup>). SMEI include missense, nonsense, frameshift mutations more commonly arising de novo affected patients. This finding is difficult to reconcile family history GEFS<sup>+</sup> a significant proportion patients SMEI. Infantile spasms (IS), or West syndrome, epileptic...
Cytomegalovirus (CMV) infection is a common in adults (seropositive 60–99% globally), and associated with cardiovascular diseases, line risk factors such as hypertension atherosclerosis. Several viral infections are linked to hypertension, including human herpes virus 8 (HHV-8) HIV-1. The mechanisms of how contributes or increased blood pressure not defined. In this report, the role CMV cause forming aortic atherosclerotic plaques examined. Using vivo mouse model vitro molecular biology...
Aequorin was used as an indicator of cytoplasmic Ca2+ levels during the action vasodilators in potassium‐depolarized strips ferret portal vein. Moderate concentrations isoprenaline produced either no change or increase smooth muscle relaxation. Only presence very high (greater than 10(‐4) M) a decrease intracellular detectable. Both papaverine and forskolin also caused relaxations while were observed not to increase. When muscles relaxed by decreasing calcium concentration bathing medium...
Studies have shown that fish oils, containing n-3 fatty acids, protective effects against ischemia-induced, fatal cardiac arrhythmias in animals and perhaps humans. In this study we used the whole-cell voltage-clamp technique to assess of dietary, free long-chain acids on Na + current (I Na,α ) human embryonic kidney (HEK293t) cells transfected with α-subunit channel (hH1 α ). Extracellular application 0.01 30 μM eicosapentaenoic acid (EPA, C20:5n-3) significantly reduced I an IC 50 0.51 ±...
The purpose of this study was to test the hypothesis that abnormal intracellular calcium handling characterizes myocardial stunning. Isolated, isovolumic, buffer-perfused ferret hearts were loaded with bioluminescent indicator aequorin for simultaneous measurement individual transients and left ventricular pressure. After 15 minutes global ischemia 20 reperfusion, developed pressure significantly reduced (75 +/- 7 versus 93 6 mm Hg, p < 0.05). During ischemia, [Ca2+]i levels elevated...
To investigate the mechanism of impaired myocardial function after long-term pressure overload, we studied cardiac muscle mechanical contraction and intracellular calcium transients using bioluminescent indicator aequorin. Left ventricular papillary preparations were examined from three groups rats: 1) aging spontaneously hypertensive rats (SHR) with clinical pathological evidence suggesting heart failure (SHR-F group), 2) age-matched SHRs no (SHR-NF 3) normotensive Wistar-Kyoto (WKY group)....
Neuregulins and their erbB receptors are essential for cardiac development postulated to be cardioprotective in the presence of injury postnatal heart. We tested hypothesis that doxorubicin-induced cardiotoxicity vivo is more severe mice with heterozygous knockout neuregulin-1 gene (NRG-1 +/− ) compared wild-type (WT). Three-month old NRG-1 WT were injected a single dose doxorubicin (20 mg/kg ip). Survival was analyzed by Kaplan-Meier approach. Left ventricular (LV) function signaling...
range (mean ± 2 standard deviations) for age each immunoglobulin is given in parentheses and derived from the data of Allanamith, McClellan, Butter- worth, Maloney (1968).
Previous epidemiologic data demonstrate that cardiovascular (CV) morbidity and mortality may occur decades after ionizing radiation exposure. With increased use of proton carbon ion radiotherapy concerns about space exposures to astronauts on future long-duration exploration-type missions, the long-term effects risks low-dose charged particle irradiation CV system must be better appreciated. Here we report whole-body (1H; 0.5 Gy, 1 GeV) iron (56Fe; 0.15 1GeV/nucleon) with without an acute...
Despite considerable advances in medicine, the incidence of heart failure remains high patients after myocardial infarction (MI). This study investigated effects engrafted early-differentiated cells (EDCs) from mouse embryonic stem cells, with or without transfection vascular endothelial growth factor (VEGF) cDNA (phVEGF(165)), on cardiac function postinfarcted mice. EDCs were transfected green fluorescent protein (GFP) and transplanted into infarcted myocardium. Compared MI mice receiving...
Na(+)/H(+) exchange inhibition with HOE642 (cariporide) improves postischemic recovery of cardiac function, but the mechanisms action remain speculative. Because is activated on reperfusion, it was hypothesized that its delays realkalinization and decreases intracellular Na(+) and, via Na(+)/Ca(2+) exchange, Ca(2+) overload. Attenuated overload prolonged acidosis are known to be cardioprotective.Left ventricular developed end-diastolic pressures were measured in isolated buffer-perfused rat...
Recent studies have established the ability of human B lymphocytes to undergo G1-phase cell cycle progression and subsequent DNA synthesis upon exposure factor(s) present in media conditioned by lectin-stimulated mononuclear cells. Procedures for isolation such a cytokine been focus investigation. Conditioned medium from cells stimulated lectin 72 hr was fractionated ammonium sulfate precipitation, ion-exchange chromatography, gel filtration chromatography. During procedure...
Background and Purpose— Stroke can lead to cerebrogenic cardiac arrhythmias. We sought investigate the effect of ischemic stroke on function in a mouse model permanent middle cerebral artery occlusion (pMCAO). Methods— Twenty-four hours after induction focal ischemia, was measured mice by endovascular catheterization heart. Immediately hemodynamic measurements, were euthanized brains excised sectioned measure infarct volume severity insular cortex injury. Myocardial damage evaluated...
Neuregulin-1 (NRG1) is a potential therapeutic agent for the treatment of doxorubicin (Dox)-induced heart failure. NRG1, however, activates erbB2 receptor, which frequently overexpressed in breast cancers. It is, therefore, important to understand how via erbB2, protects against Dox cardiotoxicity. Here, we studied NRG1-erbB2 signaling Dox-treated mice hearts and isolated neonatal rat ventricular myocytes (NRVM). Male C57BL/6 were treated with recombinant NRG1 before daily after single dose...