A5025004801- Transplantation: Methods and Outcomes
- Oral microbiology and periodontitis research
- Knee injuries and reconstruction techniques
- Sports injuries and prevention
- Mycobacterium research and diagnosis
- Helicobacter pylori-related gastroenterology studies
- Protein Tyrosine Phosphatases
- Polyomavirus and related diseases
- Renal Transplantation Outcomes and Treatments
- Eosinophilic Esophagitis
- Osteoarthritis Treatment and Mechanisms
- Galectins and Cancer Biology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lower Extremity Biomechanics and Pathologies
- Streptococcal Infections and Treatments
- Cellular Mechanics and Interactions
- Bacterial Genetics and Biotechnology
- Total Knee Arthroplasty Outcomes
- Pneumocystis jirovecii pneumonia detection and treatment
- Chronic Myeloid Leukemia Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Bacterial Identification and Susceptibility Testing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Organ and Tissue Transplantation Research
- Gastrointestinal disorders and treatments
Duke University Hospital
2021-2025
Duke University
2020-2025
Duke Medical Center
2025
Stanford University
2004-2024
Babraham Institute
2021-2024
Bioengineering Center
2022
Northeastern University
2022
St. John's University
2022
Loyola University Medical Center
2017-2019
Loyola University Chicago
2019
Acquisition of Helicobacter pylori occurs mainly in childhood. However, the mode transmission remains unclear. To help elucidate this, 100 children attending for upper gastrointestinal endoscopy were investigated presence H. at various sites. was detected antral gastric biopsies by rapid urease test (13 patients), culture histology (15 patients) and PCR (20 patients). Gastric juice positive 3 patients 11 PCR. The dental plaque from 68% biopsy-positive (as determined or PCR) 24%...
The prevailing circuit model predicts that hyperactivity of the striatopallidal pathway and subsequently increased inhibition external globus pallidus (GPe) neurons lead to hypokinetic symptoms Parkinson's disease (PD). It is believed due inactivity dopamine receptors on somatodendritic membrane neurons, but exact cellular underpinnings remain unclear. In this study, we show mouse GPe astrocytes critically control ambient glutamate level, which in turn gates transmission via activation...
Abstract Introduction Previous cardiothoracic surgery (CTS) is associated with a significant risk of perioperative bleeding in lung transplantation (LT). The types prior have not been well‐defined. We aimed to quantify the LT based on history previous CTS. Methods conducted retrospective study adult patients who underwent bilateral and stratified recipients into no CTS (No‐CTS), minimally invasive (Mi‐CTS), or open/invasive (I‐CTS). primary outcome was occurrence severe/massive worse by...
The arginine‐specific protease activity of Porphyromonas gingivalis is considered to be an important factor in the pathogenic potential this organism destructive periodontal disease. Multiple forms closely related Arg‐x proteases are present culture supernatants P. W50. RI a heterodimer (α/β) which catalytic α chain associated with second β functions as haemagglutinin. RIA single‐chain enzyme (α) and RIB highly post‐translationally lipid‐modified (LPS‐α) reduced solubility compared other two...
BACKGROUND: There are alterations in strength relation to menstrual cycle phase but little data attributing these responses female sex hormone levels using a pseudo-menstrual group as control. OBJECTIVE: Examining the effects of
Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked phosphatases (RPTPs) receive cues from extracellular environment are well placed to influence cell signaling. However, direct events downstream these receptors have been challenging resolve. We report here that homophilic receptor PTPRK is stabilized at cell-cell contacts epithelial cells. By combining interaction studies, quantitative...
ABSTRACT PTPRK is a receptor tyrosine phosphatase that linked to the regulation of growth factor signalling and tumour suppression. It stabilized at plasma membrane by trans homophilic interactions upon cell–cell contact. regulates adhesion but also reported regulate numerous cancer-associated pathways. However, mechanism remains be determined. Here, we find cell signalling, suppresses invasion promotes collective, directed migration in colorectal cancer cells. In vivo, supports recovery...
The prpR1 gene of Porphyromonas gingivalis W50 encodes the polyprotein precursor (PrpRI) an extracellular arginine-specific protease. PrpRI is organized into four distinct domains (pro, alpha, beta, and gamma) processed to a heterodimeric protease (RI) which comprises alpha beta components in noncovalent association. component contains active site, whereas appears have role adherence hemagglutination processes. DNA sequences homologous coding region for RI are present at multiple loci on P....
Helicobacter pylori is rarely cultured from sites other than the gastric mucosa. The morphology of H. in stomach and dental plaque adult dyspeptic patients was investigated to determine whether a difference at these could explain inability culture organism oral cavity. Five attending for an upper gastrointestinal endoscopy were investigated. Dental antral biopsy samples analysed by polymerase chain reaction (PCR) both before after immunomagnetic separation using polyclonal rabbit anti-H....
In a previous investigation of the laboratory identification clinical strains genus Peptostreptococcus, several isolates were found to be atypical. this study, we further examined these by using both phenotypic and genotypic methods. Based on our findings, describe following three new species Peptostreptococcus from human specimens: harei, whose type strain is DSM 10020 (isolated sacral sore); ivorii, 10022 leg ulcer); octavius, NCTC 9810 nasal flora). An analysis their 16S rRNA gene...
The prevalence and populations of Porphyromonas Prevotella species were determined in three samples dental plaque from each 34 healthy dogs. gingivalis was present 68 per cent the dogs 47 samples. counts P increased with age (P < 0.1), amount 0.05) degree gingivitis 0.1). intermedia 44 23 correlated canoris, salivosa, cangingivalis, cansulci, crevioricanis denticola isolated only 9, 6, 3, 3 dogs, respectively. gingivicanis not any animals sampled. There a highly significant 0.001)...
The transmission of Helicobacter pylori may occur by spread organisms from gastric juice which has been introduced into the mouth gastro-oesophageal reflux. aim this study was to quantify load H. present in available for transmission. Gastric antral biopsy and samples were collected 108 adult dyspeptic patients undergoing routine upper gastroscopy presence determined. In all, 54 (50%) gave positive results rapid urease test histology. 40 (37%) A gene PCR assay; 34 (31%) these three tests...
Morbidity associated with femur fractures in polytrauma patients is known to be high. The many unsolved clinical questions include the immunological effect of fracture and its fixation, timing management non-union, infection critical size bone defects. aim this study was establish a clinically-relevant reproducible animal model regards histological, biomechanical radiological changes during healing. A custom-designed intramedullary nail interlocking system (RabbitNail, RISystem AG, Davos...
Animal bite wounds are amongst the most common types of traumatic injuries in humans. The organisms isolated from these generally reflect oral flora biting animal, and may be fastidious nature difficult to identify. This study was undertaken determine prevalence Eikenella corrodens, Actinobacillus actinomycetemcomitans, Porphyromonas Prevotella spp. supragingival dental plaque collected right maxillary canine carnassial teeth mandibular tooth dogs. In part one study, 30 dogs were used. E....
Background Injuries to the articular cartilage in knee are common jumping athletes, particularly high‐level basketball players. Unfortunately, these often diagnosed at a late stage of disease process, after tissue loss has already occurred. Purpose/Hypothesis To evaluate longitudinal changes and function National Collegiate Athletic Association (NCAA) players their evolution over competitive season off‐season. Study Type Longitudinal, multisite cohort study. Population Thirty‐two NCAA...
ABSTRACT The prpR1 of Porphyromonas gingivalis codes for three distinct enzymes with specificity arginyl peptide bonds termed RI, RIA, and RIB. These isoforms comprise the majority extracellular, arginine-specific protease activity in P. W50. RI is a heterodimer which catalytic α chain noncovalently associated second involved adherence phenomena. RIA RIB are both monomeric species. represents free chain, highly posttranslationally modified form exclusively vesicle or membrane migrates as...
Abstract Magnetic resonance imaging (MRI) is commonly used to evaluate the morphology of knee in athletes with high‐knee impact; however, complex repeated loading joint can lead biochemical and structural degeneration that occurs before visible morphological changes. In this study, we utilized multiparametric quantitative MRI compare composition articular cartilage subchondral bone shape between young impact (basketball players; n = 40) non‐knee (swimmers; 25). We implemented voxel‐based...