A5102730817- Oral microbiology and periodontitis research
- Oral Health Pathology and Treatment
- HIV/AIDS oral health manifestations
- Streptococcal Infections and Treatments
- Oral and gingival health research
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Salivary Gland Disorders and Functions
- Rheumatoid Arthritis Research and Therapies
- Neonatal and Maternal Infections
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Inflammatory mediators and NSAID effects
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Immune Response and Inflammation
- Drug-Induced Adverse Reactions
- Probiotics and Fermented Foods
- Machine Learning in Bioinformatics
King's College London
2019-2024
Guy's Hospital
2019
Queen Mary University of London
1997
One of the hallmark features destructive periodontal disease, well documented over last 50 y, is a change to quantitative and qualitative composition associated microbiology. These alterations are now generally viewed as transformational shifts microbial populations with health leading emergence bacterial species, which only present in low abundance proportionate decrease others. The role this dysbiosis microbiota development disease remains controversial: altered microbiology driving agent...
The arginine‐specific protease activity of Porphyromonas gingivalis is considered to be an important factor in the pathogenic potential this organism destructive periodontal disease. Multiple forms closely related Arg‐x proteases are present culture supernatants P. W50. RI a heterodimer (α/β) which catalytic α chain associated with second β functions as haemagglutinin. RIA single‐chain enzyme (α) and RIB highly post‐translationally lipid‐modified (LPS‐α) reduced solubility compared other two...
Cargo proteins of the type IX secretion system (T9SS) in human pathogens from Bacteroidetes phylum invariably possess a conserved C-terminal domain (CTD) that functions as signal for outer membrane (OM) translocation. In Porphyromonas gingivalis, CTD cargos is cleaved off after translocation, and anionic lipopolysaccharide (A-LPS) attached. This transpeptidase reaction anchors secreted to OM. PorZ, cell surface-associated protein, an essential component T9SS whose function was previously...
A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The comprises collection nearly full-length sequences from cultured isolates draft genomes representative taxa collected range sources, including specific-pathogen-free laboratory mice, wild Mus musculusdomesticus formerly wood mouse Apodemus sylvaticus At present, it 103 (MOT) spanning...
Rheumatoid arthritis (RA), a chronic inflammatory disease affecting primarily the joints, is frequently characterized by presence of autoimmune anticitrullinated protein antibodies (ACPA) during preclinical stages and accumulation hypercitrullinated proteins in arthritic joints. A strong association has been reported between RA periodontal disease, Porphyromonas gingivalis, known driver periodontitis, proposed as microbial link underlying this association. We recently demonstrated P....
Periodontal disease is accompanied by alterations to cellular profiles and biological activities of both the subgingival microbiome host tissues. Although significant progress has been made in describing molecular basis homeostatic balance host–commensal microbe interactions health compared destructive imbalance disease, particularly with respect immune inflammatory systems, few studies have attempted a comprehensive analysis diverse models. Here, we describe development application...
Abstract The periodontal pathogen Porphyromonas gingivalis is genetically heterogeneous. However, the spontaneous generation of phenotypically different sub‐strains has also been reported. McKee et al. (1988) cultured P. W50 in a chemostat during investigations into growth and properties this bacterium. Cell viability on blood agar plates revealed two types non‐pigmenting variants, beige (BE1), brown (BR1), samples grown high‐hemin medium after day 7, population these variants increased to...
Periodontal disease is considered to arise from an imbalance in the interplay between host and its commensal microbiota, characterized by inflammation, destructive periodontal bone loss, a dysbiotic oral microbial community. The neutrophil key component of defense periodontium: defects their number or efficacy function predisposes individuals development disease. Paradoxically, activity, as part deregulated inflammatory response, important element process. In this investigation, we examined...
Porphyromonas gingivalis is a gram-negative oral anaerobic pathogen and one of the key causative agents periodontitis. P. utilises range virulence factors, including cysteine protease RgpB, to drive pathogenesis these are exported attached cell surface via type IX secretion system (T9SS). All cargo proteins possess conserved C-terminal signal domain (CTD) which recognised by T9SS, outer membrane β-barrel protein PorV (PG0027/LptO) can interact with as they bacterial surface. Using...
It is presently unclear why there a high prevalence of periodontal disease in individuals living with chronic kidney (CKD). Whilst some have argued that causes CKD, we hypothesised alterations saliva and the oral microenvironment organisms may initiate by causing dysbiosis microbiota.
Abstract Cargo proteins of the type IX secretion system (T9SS) in human pathogens from phylum Bacteroidetes invariably possess a conserved C-terminal domain (CTD) that functions as signal for outer membrane (OM) translocation. In Porphyromonas gingivalis , CTD selected cargos is cleaved off after translocation, and anionic lipopolysaccharide (A-LPS) attached. This transpeptidase reaction anchors secreted to OM. PorZ, cell surface-associated protein, an essential component T9SS whose function...
Abstract It is presently unclear why there a high prevalence of periodontal disease (PD) in individuals living with chronic kidney (CKD). By employing three different models rats and mice, we demonstrate that experimental uremia causes bone loss. Uremia alters the biochemical composition saliva induces progressive dysbiosis oral microbiota, microbial samples from uremic animals displaying reduced overall bacterial growth, increased alpha diversity, abundance key components healthy microbiota...
Periodontal disease is a chronic inflammatory in which the oral pathogen
Abstract Periodontal disease is a common chronic inflammatory disease. Porphyromonas gingivalis an important bacterium in the development of and expresses variety virulence determinants. Hemin (iron [III] protopotphyrin IX), essential nutrient this organism, whose concentration increases with increasing inflammation, global regulator P. : high hemin levels increase expression several However, mechanism through which influences bacterial gene poorly understood. Bacterial DNA methylation has...