The advent of on-line multidimensional liquid chromatography-mass spectrometry has significantly impacted proteomic analyses complex biological fluids such as plasma. However, there is general agreement that additional advances to enhance the peak capacity platforms are required accuracy and coverage proteome maps fluids. Here, we describe combination strong-cation-exchange reversed-phase chromatographies with ion mobility mass a means characterizing mixture proteins associated human plasma...

The synthesis of increasingly complex polymers has created daunting, sometimes insurmountable problems for their chemical analysis. importance is magnified by outsourcing production and use in consumer products, including medical devices food storage, therefore requires a new generation technology quality assurance. Here, we report capturing subtle differences at the molecular level polymer mixtures nearly instantaneously using prototype multidimensional ion mobility spectrometry/mass...

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTMass Spectrometry of Synthetic PolymersSteffen M. Weidner† and Sarah Trimpin*‡View Author Information Federal Institute for Materials Research Testing (BAM), D-12489 Berlin, Richard-Willstaetter-Strasse 11, Germany, Department Chemistry, Wayne State University, 5101 Cass Avenue, 33 Detroit, Michigan 48202* To whom the correspondence should be addressed. E-mail: [email protected]†Federal (BAM).‡Wayne University.Cite this: Anal. Chem. 2010, 82, 12,...

Ion mobility and mass spectrometry techniques, combined with electrospray ionization, have been used to examine distributions of poly(ethylene glycols) (PEG) average molecular masses 6550 17900 Da. The analysis provides information about the polymer size as well smaller oligomers existing over a wide range charge states sizes (i.e., [HO(CH2CH2O)xH + nCs]n+, where x ranges from 21 151 n = 2 11 for Da sample; and, 362 23 17 900 sample). present data show that oligomer also fall into families,...

The first example of a matrix-assisted laser desorption/ionization (MALDI) process producing multiply charged mass spectra nearly identical to those observed with electrospray ionization (ESI) is presented. MALDI noted for its ability produce singly ions, but in the experiments described here ions are produced by ablation analyte incorporated into common matrix, 2,5-dihydroxybenzoic acid, using standard solvent-based sample preparation protocols. Laser known matrix clusters provided...

Mechanistic arguments relative to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) address observations that predominately singly charged ions are detected. However, recently a matrix assisted ablation method, laserspray ionization (LSI), was introduced can use the same sample preparation and as MALDI, but produce highly from proteins. In generated neutral molecules by photon energy provided matrix, while in LSI produced inside heated inlet tube linking atmospheric...

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTAdvances in Ionization for Mass SpectrometryPatricia M. Peacock†, Wen-Jing Zhang‡, and Sarah Trimpin*‡View Author Information† First State IR, LLC, 118 Susan Drive, Hockessin, Delaware 19707, United States‡ Department of Chemistry, Wayne University, 5101 Cass Avenue, Detroit, Michigan 48202, States*E-mail: [email protected]Cite this: Anal. Chem. 2017, 89, 1, 372–388Publication Date (Web):November 15, 2016Publication History Published online6...

A method of solvent-free sample preparation is shown to be universal applicability for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Results obtained were compared with those traditional solvent-based MALDI-MS in order demonstrate their similarities respect accuracy, sensitivity and resolution polymers such as polystyrene poly(methyl methacrylate) a range from 2 100 kDa. The results revealed that there fundamentally no difference the quality spectra, we conclude...

A dry sample preparation strategy was previously established as a new method for matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), so-called solvent-free MALDI-MS. In this contribution, we examine systems that have been shown problematic with conventional solvent-based MALDI approaches. Problems frequently encountered are solubility, miscibility, and segregation effects during crystallization result of unfavorable analyte polarities. all cases studied, MALDI-MS...

Laserspray ionization (LSI) is a new approach to producing multiply charged ions from solids on surfaces by laser ablation of matrixes commonly used in matrix-assisted desorption/ionization (MALDI). We show that the only necessity for deliver particles or droplets matrix/analyte mixture an zone which simply heated inlet vacuum mass spectrometer. Several other methods delivering sample are demonstrated produce nearly equivalent results. One example shows use air gun replacing and spectra...

An astonishingly simple new method to produce gas-phase ions of small molecules as well proteins from the solid state under cold vacuum conditions is described. This matrix assisted ionization (MAIV) mass spectrometry (MS) produces multiply charged similar those that typify electrospray (ESI) and uses sample preparation methods are nearly identical matrix-assisted laser desorption/ionization (MALDI). Unlike these established methods, MAIV does not require a or voltage for ionization, unlike...

An extra dimension of polymer analysis: ion mobility spectrometry-mass spectrometry (IMS-MS) separates ions according to their size in the gas phase, allowing differentiation linear and cyclic polymeric isomers. This analytical technique is a rapid sensitive method for assessing purity one step. As highly pure polymers are crucial adequately architecture dependent properties, IMS–MS offers great promise characterization this unique class polymers.

The introduction of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) for the mass spectrometric analysis peptides proteins had a dramatic impact on biological science. We now report that wide variety compounds, including peptides, proteins, protein complexes, are transported directly from solid-state small molecule matrix to gas-phase ions when placed into vacuum spectrometer without use high voltage, laser, or added heat. This process produces having...

The systematic study of the temperature and pressure dependence matrix-assisted ionization (MAI) led us to discovery seemingly impossible, initially explained by some reviewers as either sleight hand or misinterpretation an overzealous young scientist results reported many years before having little utility. "magic" that we were attempting report was with matrix assistance, molecules, at least large bovine serum albumin (66 kDa), are lifted into gas phase multiply charged ions simply...

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTMass Spectrometry of Synthetic PolymersSteffen M. Weidner† and Sarah Trimpin*‡View Author Information Federal Institute for Materials Research Testing (BAM), D-12489 Berlin, Richard-Willstaetter-Strasse 11, Germany, Department Chemistry, Wayne State University, 5101 Cass Avenue, 33 Detroit, Michigan 48202* To whom the correspondence should be addressed. E-mail: [email protected]†Federal (BAM).‡Wayne University.Cite this: Anal. Chem. 2008, 80, 12,...

Matrix assisted inlet ionization (MAII) is a method in which matrix:analyte mixture produces mass spectra nearly identical to electrospray without the application of voltage or use laser as required laserspray (LSI), subset MAII. In MAII, sample introduced by, for example, tapping particles dried into spectrometer and, therefore, permits study conditions pertinent formation multiply charged ions need absorption at wavelength. Crucial production highly are desolvation remove matrix molecules...

Matrix-assisted ionization (MAI)-mass spectrometry (MS) eliminates the need for high voltage, a heat source, lasers, and compressed gases in process uses minimal solvents sample preparation, thus making MAI ideal field-portable mass spectrometers. The broad applicability of is demonstrated by simple, rapid, robust positive negative detection mode analyses low compounds including some pesticides, dyes, drugs, lipids, proteins (186 Da to 8.5 kDa) from various materials urine, biological tissue...

The application of solvent-free sample preparation for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allowed the characterization an insoluble fraction poly(9,9-diphenylfluorene) that was previously hindered by lack suitable methods. MALDI spectrometric analysis gives valuable mechanistic information about heterogeneous polymerization process high molecular weight polymer. fragmentation appearing even under moderate desorption and ionization...

Laserspray ionization (LSI) mass spectrometry (MS) allows, for the first time, analysis of proteins directly from tissue using high performance atmospheric pressure spectrometers. Several abundant and numerous lower protein ions with molecular masses up to ∼20,000 Da were detected as highly charged delipified mouse brain mounted on a common microscope slide coated 2,5-dihydroxyacetophenone matrix. The ability LSI produce multiply by laser ablation at allowed 100,000 resolution an Orbitrap...

The ability to analyze complex (macro) molecules is of fundamental importance for understanding chemical, physical, and biological processes. Complexity may arise from small differences in structure, large dynamic range, as well a vast range solubility or ionization, imposing daunting tasks areas different lipidomics proteomics. Here, we describe rapid matrix application that permits the deposition matrix-assisted laser desorption/ionization (MALDI) solvent-free. This solvent-free one-step...

Multiply charged ions, similar to those obtained with electrospray ionization, are produced at atmospheric pressure (AP) using standard MALDI conditions of laser fluence and reflective geometry. Further, the charge state can be switched singly ions nearly instantaneously by changing voltage applied target plate. Under normal AP-MALDI operating in which a is plate, primarily observed, but or near zero volts, highly observed for peptides proteins. Thus, switching between multiply requires only...