A5102260080- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- HIV/AIDS drug development and treatment
- Cell death mechanisms and regulation
- Genetic and Kidney Cyst Diseases
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- interferon and immune responses
- Sarcoma Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Tumors and Oncological Cases
- Animal Virus Infections Studies
- Neuroscience and Music Perception
- Poxvirus research and outbreaks
- Abdominal Trauma and Injuries
- Polyomavirus and related diseases
- Organ Transplantation Techniques and Outcomes
- Drug-Induced Hepatotoxicity and Protection
- Soft tissue tumor case studies
- NF-κB Signaling Pathways
- Immunotherapy and Immune Responses
- Hepatitis Viruses Studies and Epidemiology
Konkuk University
2010-2024
Bio-Medical Science (South Korea)
2023
Jeonbuk National University
2023
Objective Interferons (IFNs) mediate direct antiviral activity. They play a crucial role in the early host immune response against viral infections. However, IFN therapy for HBV infection is less effective than other Design We explored cellular targets of to IFNs using proteome-wide screening. Results Using LC-MS/MS, we identified proteins downregulated and upregulated by treatment X protein (HBx)-stable control cells. found several IFN-stimulated genes HBx, including TRIM22 , which known as...
Liver regeneration after liver damage caused by toxins and pathogens is critical for homeostasis. Retardation of proliferation was reported in hepatitis B virus (HBV) X protein (HBx)-transgenic mice. However, the underlying mechanism HBx-mediated disturbance unknown. We investigated molecular inhibition using cell lines a mouse model. The model acute HBV infection established hydrodynamic injection viral DNA. partial hepatectomy significantly inhibited DNA-treated Mechanism studies have...
Cytokines are involved in early host defense against pathogen infections. In particular, tumor necrosis factor (TNF) and interferon-gamma (IFN-γ) have critical functions non-cytopathic elimination of hepatitis B virus (HBV) hepatocytes. However, the molecular mechanisms mediator molecules largely unknown. Here we show that interleukin-32 (IL-32) is induced by TNF IFN-γ hepatocytes, inhibits replication HBV acting intracellularly to suppress transcription replication. The gamma isoform IL-32...
ABSTRACT Clevudine (CLV) is a nucleoside analog with potent antiviral activity against chronic hepatitis B virus (HBV) infection. Viral resistance to CLV in patients receiving therapy has not been reported. The aim of this study was characterize CLV-resistant HBV viral breakthrough (BT) during long-term therapy. gene encoding reverse transcriptase (RT) analyzed from BT Sera collected the at baseline and time were studied. To mutations isolated patients, we subjected mutants vitro drug...
The emergence of drug-resistant hepatitis B virus (HBV) is a major problem for antiviral treatment in chronic infection. In this study, we analyzed the evolution mutations and characterized effects rtA181T rtI233V on viral replication drug resistance. We performed clonal analysis HBV polymerase gene from serum samples during breakthrough treated with agents. A series mutant clones containing and/or were constructed determined effect these ability An vitro study revealed that mutation...
Smallpox, caused by the variola virus belonging to genus Orthopoxvirus, is an acute contagious disease that killed 300 million people in 20th century. Since it was declared be eradicated and national immunization program against stopped, has become a prospective bio-weapon. It necessary develop safe vaccine protects from terrorism using this biological weapon can administered immunocompromised people. Our previous study reported on development of attenuated smallpox (KVAC103). This evaluated...
Sustained activation of NF-κB is one the causative factors for various liver diseases, including inflammation and hepatocellular carcinoma (HCC). It has been known that activating signal by hepatitis B virus X protein (HBx) implicated in development HCC. However, despite numerous studies on HBx-induced activation, detailed mechanisms still remain unsolved. Recently, p22-FLIP, a cleavage product c-FLIPL, reported to induce through interaction with IκB kinase (IKK) complex primary immune...
The emergence of compensatory mutations in the polymerase gene drug resistant hepatitis B virus (HBV) is associated with treatment failure. We previously identified a multi-drug HBV mutant, which displayed resistance towards lamivudine (LMV), clevudine (CLV), and entecavir (ETV), along strong replication capacity. aim this study was to identify unknown mutations, determine clinical relevance mutation during antiviral therapy. In vitro mutagenesis, susceptibility assay, molecular modeling...
Hand-foot-and-mouth disease (HFMD) is a viral infectious that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there no efficient therapeutics for HFMD, vaccines effective preventing the disease. To develop broad coverage against CV EV, development bivalent vaccine form needed. The Mongolian gerbil an suitable animal model EV71 C4a CVA16 infection used to investigate efficacy following direct immunization. In this study, gerbils...
In chronic hepatitis B virus (HBV) infection, quantitative HBV surface antigen (qHBsAg) is useful for monitoring viral replication and treatment responses. We aimed to determine whether pre-S mutations have any effect on circulating qHBsAg.Plasmids expressing 1–8 amino acid deletion in pre-S1 ("pre-S1Δ1-8") 3-25 pre-S2 ("pre-S2Δ3-25") were constructed. At 72 h posttransfection into Huh7 cells, qHBsAg measured using electrochemiluminescence immunoassay analyzer. To mimic milieus of...
See the commentary-article "Is tenofovir based therapy almighty for previous treatment failure in chronic hepatitis B?" on page 238.
Direct sequencing is the gold standard for detection of drug-resistance mutations in hepatitis B virus (HBV); however, this procedure time-consuming, labor-intensive, and difficult to adapt high-throughput screening. In study, we aimed develop a dendron-modified DNA microarray genotypic resistance evaluate its efficiency. The specificity, sensitivity, selectivity slides representative were evaluated compared those conventional slides. diagnostic accuracy was validated using sera obtained...
Abstract Background and Aim Since polymerase surface genes overlap in hepatitis B virus (HBV), an antiviral‐induced mutation the gene may alter antigenicity patients with chronic (CHB), but this possibility has not been clearly confirmed. This study aimed to determine drug susceptibility of patient‐derived mutants. Patients Methods Full‐length HBV genomes isolated from four entecavir‐resistant CHB were cloned sequenced. Around 10 clones full‐length obtained each patient analysed registered...
Hepatitis B virus (HBV) is a sex-specific pathogen that more severe in males than females. Sex disparities HBV infection have been attributed to hormonal differences between and However, whether affects the metabolic signatures of steroid hormones how these influences viral replication remains unclear. In this study, we investigated alters metabolism its effects on replication. Serum samples from male female mice obtained after hydrodynamic injection replication-competent plasmids were...