A5087809978- Influenza Virus Research Studies
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Protein Structure and Dynamics
- Viral gastroenteritis research and epidemiology
- Heat shock proteins research
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Tea Polyphenols and Effects
- Bacterial Genetics and Biotechnology
- Toxin Mechanisms and Immunotoxins
- Virus-based gene therapy research
- Protein purification and stability
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Synthesis and Analysis
- Viral Infections and Immunology Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
Yonsei University
2015-2024
Government of the Republic of Korea
2020
Weatherford College
2010-2013
Protein Express (United States)
2012
Howard Hughes Medical Institute
2006
Yale University
2006
Konkuk University
2006
Kyungpook National University
2006
Korea Institute of Science and Technology
1981-2006
Green Cross (South Korea)
1996
Here, we report for the first time cell-permeable and biocompatible polymeric nanoparticles consisting of a polymer conjugated to near-infrared (NIR) fluorescence (Cy5.5)-linked effector caspase-specific peptide. The close spatial proximity NIR fluorochromes in results an autoquenched state, but give rise strong signal under apoptotic cells. Thus, smart nanoparticle developed here is attractive probe real-time imaging apoptosis single
Type I interferon (IFN-I) plays a critical role in the homeostasis of hematopoietic stem cells and influences neutrophil influx to site inflammation. IFN-I receptor knockout (Ifnar1−/−) mice develop significant defects infiltration Ly6Chi monocytes lung after influenza infection (A/PR/8/34, H1N1). wild-type (WT) are main producers MCP-1 while alternatively generated Ly6Cint Ifnar1−/− mainly produce KC for influx. As consequence, recruit more neutrophils than do WT mice. Treatment IFNAR1...
The folding of monomeric antigens and their subsequent assembly into higher ordered structures are crucial for robust effective production nanoparticle (NP) vaccines in a timely reproducible manner. Despite significant advances silico design structure-based assembly, most engineered NPs refractory to soluble expression fail assemble as designed, presenting major challenges the manufacturing process. failure is due lack understanding kinetic pathways enabling technical platforms ensure...
We have generated mutants of Escherichia coli formylmethionine initiator tRNA in which one, two, and all three G X C base pairs the GGGCCC sequence anticodon stem are changed to those found E. elongator methionine tRNA. Overproduction mutant tRNAs using M13 recombinants as an expression vector development a one-step purification scheme allowed us purify, characterize, analyze function tRNAs. After aminoacylation formylation, formylmethionyl was analyzed MS2 RNA-directed vitro...
Recent studies have revealed that innate immunity is involved in the development of adaptive immune responses; however, its role protection not clear. In order to elucidate exact Toll-like receptor (TLR) or RIG-I-like (RLR) signaling on immunogenicity and protective efficacy against influenza A virus infection (A/PR/8/34 [PR8]; H1N1), we adapted several signal-deficient mice (e.g., TRIF(-/-), MyD88(-/-), MyD88(-/-) TLR3(-/-) TLR7(-/-), IPS-1(-/-)). this study, found MyD88 was required for...
Objective Interferons (IFNs) mediate direct antiviral activity. They play a crucial role in the early host immune response against viral infections. However, IFN therapy for HBV infection is less effective than other Design We explored cellular targets of to IFNs using proteome-wide screening. Results Using LC-MS/MS, we identified proteins downregulated and upregulated by treatment X protein (HBx)-stable control cells. found several IFN-stimulated genes HBx, including TRIM22 , which known as...
We show that the structure and/or sequence of first three base pairs at end amino acid acceptor stem Escherichia coli initiator tRNA and discriminator 73 are important for its formylation by E. methionyl-tRNA transformylase.This conclusion is based on mutagenesis gene followed measurement kinetic parameters mutant tRNAs in vitro function protein synthesis vivo.The pair found all other replaced a C.A "mismatch" tRNA.Mutation this to U:A, weak pair, or U.G, mismatch, has little effect...
We show that the absence of a Watson-Crick base pair at end amino acid acceptor stem, which is hallmark all prokaryotic initiator tRNAs, one key features prevents them from acting as an elongator in protein synthesis. generated mutants Escherichia coli formylmethionine tRNA have stem. The were C1----T1, had U.A pair, A72----G72, C.G and C1A72----T1G72 double mutant, lacked pair. After aminoacylation, activity these other mutant methionyl-tRNAs (Met-tRNAs) elongation assayed MS2 RNA-directed...
Pandemic influenza poses a serious threat to global health and the world economy. While vaccines are currently under development, passive immunization could offer an alternative strategy prevent treat virus infection. Attempts develop monoclonal antibodies (mAbs) have been made. However, based on mAbs may require cocktail of with broader specificity in order provide full protection since generally specific for single epitopes. Chicken immunoglobulins (IgY) found egg yolk used mainly...
Influenza virus continues to emerge and re-emerge, posing new threats for humans. Here we tested various Korean medicinal plant extracts potential antiviral activity against influenza viruses. Among them, an extract of Agrimonia pilosa was shown be highly effective all three subtypes human viruses including H1N1 H3N2 A B virus. The EC(50) value virus, as by the plaque reduction assay on MDCK cells, 14-23 microg/ml. also exhibited a virucidal effect at concentration 160-570 ng/ml when were...
Background The ectodomain of matrix protein 2 (M2e) influenza A virus is a rationale target antigen candidate for the development universal vaccine against as M2e undergoes little sequence variation amongst human strains. Vaccine-induced M2e-specific antibodies (Abs) have been shown to display significant cross-protective activity in animal models. M2e-based constructs be more protective when administered by intranasal (i.n.) route than after parenteral injection. However, i.n....
Polyphenolic compounds present in green tea, particularly catechins, are known to have strong anti-influenza activity. The goal of this study was determine whether tea by-products could function as an alternative common antivirals animals compared original tea. Inhibition viral cytopathic effects ascertained by neutral red dye uptake examined with 50% effective (virus-inhibitory) concentrations (EC50) determined. Against the H1N1 virus A/NWS/33, we found activity (EC50 = 6.36 µg/mL) be...
The non-specific effects (NSE) of vaccines have been discussed for their potential long-term beneficial beyond direct protection against a specific pathogen. Cold-adapted, live attenuated influenza vaccine (CAIV) induces local innate immune responses that provide broad range antiviral immunity. Herein, we examined whether X-31ca, donor virus CAIVs, provides cross-protection respiratory syncytial (RSV). degree RSV replication was significantly reduced when X-31ca administered before infection...
Viral infections often pose tremendous public health concerns as well economic burdens. Despite the availability of vaccines or antiviral drugs, personal hygiene is considered effective means first-hand measure against viral infections. The green tea catechins, in particular, epigallocatechin-3-gallate (EGCG), are known to exert potent activity. In this study, we evaluated extract a safe Using influenza virus A/Puerto Rico/8/34 (H1N1) model, examined duration inactivating activity (GTE)...
Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) influenza provides crucial means for immune evasion viral fitness in a host population. However, time-dependent dynamics each glycosylation sites have not been addressed. We monitored potential N-linked (NLG) over 10,000 HA NA H1N1 subtype isolated from human, avian, swine species past century. The results show shift as hallmark 1918 2009 pandemics, also 1976 “abortive pandemic”. Co-segregation particular was identified...
Abstract Membrane-disrupting agents that selectively target virus versus host membranes could potentially inhibit a broad-spectrum of enveloped viruses, but currently such antivirals are lacking. Here, we develop nanodisc incorporated with decoy receptor inhibits infection. Mechanistically, nanodiscs carrying the viral sialic acid bind to influenza virions and co-endocytosed into cells. At low pH in endosome, rupture envelope, trapping RNAs inside endolysosome for enzymatic decomposition. In...
Japanese encephalitis (JE), caused by the virus (JEV), is a highly threatening disease with no specific treatment. Fortunately, development of vaccines has enabled effective defense against JE. However, re-emerging genotype V (GV) JEV poses challenge as current are III (GIII)-based and provide suboptimal protection. Given isolation GV JEVs from Malaysia, China, Republic Korea, there concern about potential for broader outbreak. Under hypothesis that GV-based vaccine necessary JEV, we...