A5075259378- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Microbial Metabolic Engineering and Bioproduction
- Caveolin-1 and cellular processes
- Calcium signaling and nucleotide metabolism
- Enzyme Catalysis and Immobilization
- RNA Interference and Gene Delivery
- Cancer Treatment and Pharmacology
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Algal biology and biofuel production
- Influenza Virus Research Studies
- Erythrocyte Function and Pathophysiology
- CAR-T cell therapy research
- interferon and immune responses
- Biotin and Related Studies
- Antioxidant Activity and Oxidative Stress
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Structural Characterization
- Plant-based Medicinal Research
- Microtubule and mitosis dynamics
- Drug Transport and Resistance Mechanisms
- Cell death mechanisms and regulation
- Vibrio bacteria research studies
Johns Hopkins Medicine
2023-2024
Johns Hopkins University
2023-2024
Kolon Life Science (South Korea)
2020
Sungkyunkwan University
2012-2019
Qilu Hospital of Shandong University
2006
Abstract Membrane-disrupting agents that selectively target virus versus host membranes could potentially inhibit a broad-spectrum of enveloped viruses, but currently such antivirals are lacking. Here, we develop nanodisc incorporated with decoy receptor inhibits infection. Mechanistically, nanodiscs carrying the viral sialic acid bind to influenza virions and co-endocytosed into cells. At low pH in endosome, rupture envelope, trapping RNAs inside endolysosome for enzymatic decomposition. In...
Inositol pyrophosphates such as 5-diphosphoinositol pentakisphosphate (5-IP7) are highly energetic inositol metabolites containing phosphoanhydride bonds. Although known to regulate various biological events, including growth, survival, and metabolism, the molecular sites of 5-IP7 action in vesicle trafficking have remained largely elusive. We report here that elevated levels, caused by overexpression hexakisphosphate (IP6) kinase 1 (IP6K1), suppressed depolarization-induced neurotransmitter...
Anti-allergic effects of dietary polyphenols were extensively studied in numerous allergic disease models, but the molecular mechanisms anti-allergic by remain poorly understood. In present study, we show that release granular cargo molecules, contained distinct subsets granules mast cells, is specifically mediated two sets SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, and various differentially inhibit formation those complexes. Expression analysis...
Vesicle-associated V-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins and target membrane-associated T-SNAREs (syntaxin 4 SNAP-23) assemble into a core trans-SNARE complex that mediates membrane fusion during mast cell degranulation. This plays pivotal roles at various stages of exocytosis from the initial priming step to pore opening expansion, finally resulting in release vesicle contents. In this study, peptides with sequences SNARE motifs were...
Membrane fusion is mediated by the SNARE complex which formed through a zippering process. Here, we developed chemical controller for progress of membrane fusion. A hemifusion state was arrested polyphenol myricetin binds to complex. The arrest rescued an enzyme laccase that removes from metastable and long-lived with decay constant 39 min. This applied delineate how Ca(2+) stimulates fusion-pore formation in millisecond time scale. We found, using single-vesicle assay, such myricetin-primed...
Abstract Acyl myricetins (monopropionyl-, dipropionyl-, and monooctanoyl-myricetin, termed as MP 1 , 2 MO respectively) were synthesized through enzymatic or non-enzymatic esterification reaction of myricetin aglycone. Structure study indicated the hydroxyl group at C4′ in B-ring was highly susceptible to acylation. Over its parental myricetin, acylated compounds showed enhanced lipophilicity (from 7.4- 26.3-fold) oxidative stability 1.9- 3.1-fold) on basis log P decay rate, respectively....
A series of successes in RNA interference (RNAi) therapies for liver diseases using lipid nanoparticles and N-acetylgalactosamine have heralded a current era therapeutics. However, alternative delivery strategies are required to take RNAi out the comfort zone hepatocytes. Here we report SIRPα IgV/anti-CD47 siRNA (vS-siCD47) conjugates that selectively persistently disrupt antiphagocytic CD47/SIRPα axis solid tumors. Conjugation IgV domain protein siRNAs enables tumor dash through...
The objective of this study was to determine whether paclitaxel and a strong antioxidant, pyrrolidinedithiocarbamate (PDTC), can affect the activation nuclear factor-kappa B (NF-kappaB) in SKOV-3 human ovarian cancer cell line effect these two agents on growth apoptosis cells. cells were treated with various concentrations and/or PDTC at time intervals. Following treatments, determined by 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium (WST-8) (WST) assay...
Oncolytic viruses are promising cancer therapies due to their selective killing of tumor cells and ability stimulate the host immune system. As an oncolytic virus platform, vaccinia has unique advantages, including rapid replication, a broad range targets, large capacity for transgene incorporation. In this study, we developed novel with high potency favorable safety profile. We began International Health Department-White (IHD-W) strain, which had strongest cytotoxicity against among four...