A5100424011- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Respiratory viral infections research
- Immune Response and Inflammation
- Transgenic Plants and Applications
- Prostate Cancer Treatment and Research
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Vascular anomalies and interventions
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- Advanced Proteomics Techniques and Applications
- Coronary Artery Anomalies
- Protein purification and stability
- vaccines and immunoinformatics approaches
- Agriculture, Soil, Plant Science
- Wound Healing and Treatments
- Hepatitis B Virus Studies
- Immune cells in cancer
- HIV Research and Treatment
Seoul National University Bundang Hospital
2023
Yonsei University
2013-2022
Ministry of Food and Drug Safety
2021
National Institute of Food and Drug Safety Evaluation
2021
Seoul National University
2014-2021
Kyungpook National University
2014-2019
Miyata Eye Hospital
2018
Incheon National University
2018
St. Vincent's Hospital
2016
Ewha Womans University
2016
Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies shown the beneficial effects of secreted from adult stem cells, such exosomes, on skin aging. However, to date, few reports demonstrated use exosomes derived human pluripotent treatment In this study, we collected conditioned medium induced (iPSCs) investigated effect aged dermal fibroblasts (HDFs). Cell proliferation viability were determined by an MTT assay cell migration capacity was...
Cytotoxic T lymphocyte antigen-4 (CTLA-4) plays a critical role in negatively regulating cell responses and has also been implicated the development function of natural FOXP3+ regulatory cells. CTLA-4–deficient mice develop fatal, early onset lymphoproliferative disease. However, chimeric containing both –sufficient bone marrow (BM)–derived cells do not disease, indicating that CTLA-4 can act trans to maintain self-tolerance. Using genetically mixed blastocyst BM chimaeras as well vivo...
The folding of monomeric antigens and their subsequent assembly into higher ordered structures are crucial for robust effective production nanoparticle (NP) vaccines in a timely reproducible manner. Despite significant advances silico design structure-based assembly, most engineered NPs refractory to soluble expression fail assemble as designed, presenting major challenges the manufacturing process. failure is due lack understanding kinetic pathways enabling technical platforms ensure...
Secretagogin (SCGN), a Ca(2+)-binding protein having six EF-hands, is selectively expressed in pancreatic β-cells and neuroendocrine cells. Previous studies suggested that SCGN enhances insulin secretion by functioning as Ca(2+)-sensor protein, but the underlying mechanism has not been elucidated. The present study explored which glucose-induced NIT-1 insulinoma To determine whether influences first or second phase of secretion, we examined how affects kinetics We found silencing suppressed...
Juxtaposed with another zinc finger protein 1 (Jazf1) is a and known to affect both prostate cancer type 2 diabetes. Jazf1 inhibits testicular nuclear receptor 4 (TR4) activation through protein-protein interaction, which results in weight loss alleviates However, the role of still poorly understood. Hence, we investigated whether expression associated progression. We confirmed upregulation human tissue samples. In addition, using overexpressing cell lines, DU145 LNCaP, found promoted...
Abstract Prior DNA microarray studies suggested that IL-16 mRNA levels decrease following T cell activation, a property unique among cytokines. We examined pro-IL-16 and protein expression in resting anti-CD3 mAb-activated primary murine CD4+ cells. Consistent with the reports, fell within 4 h of this response is inhibited by cyclosporin A. Total cellular also fell, reaching nadir at 48 h. Pro-IL-16 comprises C-terminal cytokine domain an N-terminal prodomain are cleaved caspase-3. expressed...
Ten-eleven translocation methylcytosine dioxygenase 1 (Teil) initiates DNA demethylation by converting 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) at CpG-rich regions of genes, which have key roles in adult neurogenesis and memory. In addition, the overexpression Tet1 with 5-hmC alteration patients psychosis has also been reported, for instance schizophrenia bipolar disorders. The mechanism underlying brain; however, is still elusive. present study, we found that...
Lin28, which is highly expressed during embryogenesis, has been shown to play an important role in cell growth and embryonic development. Meanwhile, Lin28 represses let-7 miRNA biogenesis block pre-let-7 processing the cytoplasm. The family of miRNAs known repress oncogenesis cycle progression by targeting oncogenic genes signalling pathways. Consequently, acts as oncogene upregulating targets through repression biogenesis. A recent genome-wide association study (GWAS) showed that many...
Angiogenesis is an important biological process during development, reproduction, and in immune responses. Placental growth factor (PlGF) a member of vascular endothelial that critical for angiogenesis vasculogenesis. We generated transgenic mice overexpressing PlGF specifically T cells using the human CD2-promoter to investigate effects overexpression.Transgenic were difficult obtain owing high lethality; this reason, we investigated why gestational loss occurred these mice. Here, report...
To explore interactions between pilocarpine and the ROCK inhibitor, ripasudil, on IOP pupil diameter in human eyes, morphological functional changes outflow tissues vitro.IOP were measured after and/or which topically applied healthy subjects. Human trabecular meshwork (HTM) cells used a gel contraction assay, for evaluation of phosphorylation myosin light chain cofilin, immunostaining cytoskeletal proteins. Porcine ciliary muscle (CM) was CM assay. The permeability Schlemm's canal...
Live attenuated vaccine (LAV), mimicking natural infection, provides an excellent protection against microbial infection. The development of LAV, however, still remains highly empirical and the rational design clinically useful LAV is scarcely available. Apoptosis caspase activation are general host antiviral responses in virus-infected cells. Utilizing these tightly regulated defense mechanisms, we present a novel apoptosis-triggered attenuation viral virulence as live with desired levels...
Traditionally, chemical agents such as formalin (FA) and β-propiolactone (BPL) have long been used for the preparation of inactivated vaccines or toxoids. It has shown that FA extensively modifies vaccine antigens thus affects immunogenicity profiles, sometimes compromising protective efficacy even exacerbating disease upon infection. In this study, we show natural catechins from green tea extracts (GT) can be an inactivating agent to prepare viral vaccines. GT treatment resulted in complete...
Abstract Transglutaminase (TGase) induces the cross‐linking of proteins by catalyzing an acyl transfer reaction. TGase is a zymogen, activated removal its pro‐region. Because pro‐region crucial for folding and inhibition activity, recombinant expression mature (mTGase) without pro‐region, usually results in inactive inclusion bodies or low protein yield. Here, Streptomyces netropsis was fused with Escherichia coli lysyl‐tRNA synthetase (LysRS), as module chaperoning activity RNA dependent...
Solubility is the prime criterion for determining quality of recombinant proteins, yet it often fails to represent functional activity due involvement non-functional, misfolded, soluble aggregates, which compromise proteins. However, guidelines assessment proteins have neither been proposed nor rigorously validated experimentally. Using aggregation-prone enhanced green-fluorescent protein (EGFP) folding reporter system, we evaluated status by employing commonly used sonication and mild lysis...
Highly pathogenic avian influenza viruses (HPAIVs) pose a significant threat to human health, with high mortality rates, and require effective vaccines. We showed that, harnessed novel RNA-mediated chaperone function, hemagglutinin (HA) of H5N1 HPAIV could be displayed as an immunologically relevant conformation on self-assembled chimeric nanoparticles (cNP). A tri-partite monomeric antigen was designed including: i) RNA-interaction domain (RID) docking tag for RNA enable chaperna function...
This study investigates explicit and tacit knowledge acquisition influences on group reflexivity innovation behaviour in small medium-sized enterprises (SMEs) examines how relationships are moderated according to learning climate types, for example, structured or autonomous. provides a strategic view link innovative behaviour, types SME contexts. Data were derived from participants employed SMEs, enrolled the Human Resource Development Council, registered as panel informants of Korea...
Activation of different pattern recognition receptors causes distinct profiles innate immune responses, which in turn dictate the adaptive response. We found that mice had higher CD4+ T cell expansion to an immunogen, ovalbumin, when coadministered with CpG than CL097 vivo. To account for this differential adjuvanticity, we assessed activities and on antigen-specific vitro using OT-II cell/bone marrow-derived dendritic (DC) co-culture system. Unexpectedly, ovalbumin-stimulated cells was...