A5034201355- Zebrafish Biomedical Research Applications
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Neurobiology and Insect Physiology Research
- Genetics and Neurodevelopmental Disorders
- Nicotinic Acetylcholine Receptors Study
- Neurogenetic and Muscular Disorders Research
- Cardiomyopathy and Myosin Studies
- Neural dynamics and brain function
- Physiological and biochemical adaptations
- Genomics and Phylogenetic Studies
- Cellular transport and secretion
- Molecular Biology Techniques and Applications
- Retinal Development and Disorders
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Elder Abuse and Neglect
- Workplace Health and Well-being
- Single-cell and spatial transcriptomics
- Heat shock proteins research
Aoyama Gakuin University
2016-2025
Hokuriku University
2021
Pharmac
2021
University of Shiga Prefecture
2014-2018
National Institute of Genetics
2010-2017
Japan Science and Technology Agency
2011-2017
The Graduate University for Advanced Studies, SOKENDAI
2010-2017
Nagoya University
2007-2013
University of Michigan
2004-2011
Dokkyo University
2011
Transcription of messenger RNAs (mRNAs) for Notch signaling molecules oscillates with 2-hour cycles, and this oscillation is important coordinated somite segmentation. However, the molecular mechanism such remains to be determined. Here, we show that serum treatment cultured cells induces cyclic expression both mRNA protein effector Hes1, a basic helix-loop-helix (bHLH) factor, periodicity. Cycling cell-autonomous depends on negative autoregulation hes1 transcription...
The developing nervous system consists of a variety cell types. Transgenic animals expressing reporter genes in specific classes neuronal cells are powerful tools for the study network formation. We generated wide transgenic zebrafish that expressed neurons or progenitors. These include lines which neurotransmitter phenotypes fluorescent proteins Gal4, and subsets dorsal progenitor domain spinal cord proteins. Using these, we examined organization cord, found there six domains zebrafish, is...
Hes7 , a bHLH gene essential for somitogenesis, displays cyclic expression of mRNA in the presomitic mesoderm (PSM). Here, we show that protein is also expressed dynamic manner, which depends on proteasome-mediated degradation. Spatial comparison revealed and Lunatic fringe ( Lfng ) transcription occurs protein-negative domains. Furthermore, constitutively up-regulated absence down-regulated by stabilization protein. Thus, periodic repression critical this negative feedback represents...
A new monoclonal antibody (MoAb) HA58 (IgG1) was prepared, which recognizes the binding site on intercellular adhesion molecule-1 (ICAM-1) antigen to lymphocyte function-associated antigen-1 (LFA-1). The double-determinant immunoassay (DDIA) established with use of MoAb and another anti-ICAM-1, CL207, detect soluble, shedding ICAM-1 antigen. Human recombinant interferon-gamma (IFN-gamma) induced not only expression cell surface ICAM-1, but also in an IFN-gamma concentration-dependent...
A transcriptional regulator, Hes1, plays crucial roles in the control of differentiation and proliferation neuronal, endocrine, T-lymphocyte progenitors during development.Mechanisms for regulation cell by however, remain to be verified.In embryonic carcinoma cells, endogenous Hes1 expression was repressed retinoic acid concord with enhanced p27 Kip1 cycle arrest.Conversely, conditional a moderate but not maximal level HeLa cells tetracyclineinducible system resulted reduced expression,...
The potassium-chloride co-transporter KCC2, encoded by SLC12A5, plays a fundamental role in fast synaptic inhibition maintaining hyperpolarizing gradient for chloride ions. KCC2 dysfunction has been implicated human epilepsy, but to date, no monogenic KCC2-related epilepsy disorders have described. Here we show recessive loss-of-function SLC12A5 mutations patients with severe infantile-onset pharmacoresistant syndrome, of infancy migrating focal seizures (EIMFS). Decreased surface...
Mysterin, also known as RNF213, is an intracellular protein that forms large toroidal oligomers. Mysterin was originally identified in genetic studies of moyamoya disease (MMD), a rare cerebrovascular disorder unknown etiology. While mysterin to exert ubiquitin ligase and putative mechanical ATPase activities with RING finger domain two adjacent AAA+ modules, its biological role poorly understood. Here, we report targeted lipid droplets (LDs), ubiquitous organelles specialized for neutral...
Neural precursor cells proliferate in the ventricular zone while giving rise to neurons of deep layers first, then those superficial layers, and lastly, glial brain. Thus, it is essential maintain neural until late stages development for generation a wide variety cell types. Here, we found that Hes-related basic helix-loop-helix (bHLH) genes Hesr1/Hey1 Hesr2/Hey2 are expressed zone, which contains cells. Misexpression Hesr1 Hesr2 by electroporation mouse brain at embryonic day 13.5...
Wild-type zebrafish embryos swim away in response to tactile stimulation. By contrast, relatively relaxed mutants slowly due weak contractions of trunk muscles. Electrophysiological recordings from muscle showed that output the CNS was normal mutants, suggesting a defect muscle. Calcium imaging revealed Ca(2+) transients were reduced mutant fast Immunostaining demonstrated ryanodine and dihydropyridine receptors, which are responsible for release following membrane depolarization, severely...
Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin and has multiple regulatory functions in cellular redox, growth, differentiation, apoptosis, aging. To investigate the function TBP-2 vivo, we generated mice with targeted inactivation (TBP-2-/- mice). Here, show that expression markedly up-regulated during fasting wild-type mice, while TBP-2-/- were predisposed to death bleeding tendency, as well hepatic renal dysfunction result 48 h fasting. The fasting-induced...
Bilateral alternation of muscle contractions requires reciprocal inhibition between the two sides hindbrain and spinal cord, disruption this should lead to simultaneous activation bilateral muscles. At 1 day after fertilization, wild-type zebrafish respond mechanosensory stimulation with multiple fast alternating trunk contractions, whereas bandoneon ( beo ) mutants contract muscles on both simultaneously. Similar are observed in embryos treated strychnine, a blocker inhibitory glycine...
Abstract Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological behavioral traits. Motor traits, however, not explored in strains. We here applied a treadmill fish (swimmill) measured swimming capability of adult by critical speed, which is the maximum water velocity can keep swimming. First, we confirmed that does vary between female male. Second, found appropriate...
Impairment of glycinergic neurotransmission leads to complex movement and behavioral disorders. Patients harboring glycine receptor autoantibodies suffer from stiff-person syndrome or its severe variant progressive encephalomyelitis with rigidity myoclonus. Enhanced internalization was proposed as the common molecular mechanism upon autoantibody binding. Although functional impairment receptors following binding has recently been investigated, it is still incompletely understood.A cell-based...
Regulation of the intercellular adhesion molecule-1 (ICAM-1) expression on human renal tubular epithelial cells in culture (hKEC-1) was investigated. A large proportion hKEC-1 from primary cultures expressed ICAM-1 antigen. Supernatants mixed lymphocyte reaction (MLR) both specific and third-party combinations augmented antigen, a dose-dependent manner. kinetic study revealed maximal augmentation by MLR supernatant first day, with gradual decrease thereafter. Among several recombinant...
shocked ( sho ) is a zebrafish mutation that causes motor deficits attributable to CNS defects during the first2dof development. Mutant embryos display reduced spontaneous coiling of trunk, diminished escape responses when touched, and an absence swimming. A missense in slc6a9 gene encodes glycine transporter (GlyT1) was identified as cause phenotype. Antisense knock-down GlyT1 wild-type phenocopies , injection mRNA into mutants rescues them. comparison glycine-evoked inward currents Xenopus...
Mutations in the gene encoding TRPM7 ( trpm7 ), a member of Transient Receptor Potential (TRP) superfamily cation channels that possesses an enzymatically active kinase at its C terminus, cause touch-unresponsive zebrafish mutant touchdown . We identified and characterized new allele , as well two previously reported alleles, found all three alleles harbor mutations abolish channel activity. Through selective restoration expression sensory neurons, we TRPM7's activity selectivity for...
Pathogenic variants in the X-linked gene ZC4H2, which encodes a zinc-finger protein, cause an infrequently described syndromic form of arthrogryposis multiplex congenita (AMC) with central and peripheral nervous system involvement. We present genetic detailed phenotypic information on 23 newly identified families simplex cases that include 19 affected females from 18 14 males nine families. Of note, 15 deleterious de novo ZC4H2 presented phenotypes ranging mild to severe, their clinical...
ABSTRACT The 18th International Zebrafish Conference (IZFC2024) took place from August 17 to 21, 2024, at Miyako Messe in Kyoto, Japan. This conference attracted 641 researchers around the world along with 83 virtual participants, making it largest gathering since COVID‐19 pandemic. event featured two keynote lectures, three award 36 plenary talks, 90 oral presentations, and 374 poster presentations. Participants also engaged luncheons, workshops, community sessions, all aimed exploring...