A5090648674- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Phytochemicals and Medicinal Plants
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Gas Sensing Nanomaterials and Sensors
- Genetic and Environmental Crop Studies
- Animal Nutrition and Physiology
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- IL-33, ST2, and ILC Pathways
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Plant Genetic and Mutation Studies
- ZnO doping and properties
- Light effects on plants
- Medicinal Plants and Neuroprotection
- Transgenic Plants and Applications
- Lipid Membrane Structure and Behavior
- Parathyroid Disorders and Treatments
- Pharmacovigilance and Adverse Drug Reactions
- Coconut Research and Applications
- Nuclear Structure and Function
Universitat de València
2024
All India Institute of Medical Sciences Raipur
2024
Banaras Hindu University
1968-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2023
Institut de Biosciences et Biotechnologies
2023
Institute of Medical Sciences
2023
Centre National de la Recherche Scientifique
2022
Institut de Biologie Moléculaire des Plantes
2021-2022
Université de Strasbourg
2021-2022
Central Avian Research Institute
2021
A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieria trifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails some highly viscous material on walls suspension vessels or developed thick overlay semisolid media without adversities plant growth. FTIR identified this substance be an extracellular polysaccharide. Various morphological, biochemical tests, and molecular analyses using 16S rRNA, atpD, recA genes...
Background Sterol glycosyltrnasferases (SGT) are enzymes that glycosylate sterols which play important role in plant adaptation to stress and medicinally plants like Withania somnifera. The present study aims find the of WsSGTL1 is a sterol glycosyltransferase from W. somnifera, plant’s abiotic stress. Methodology gene was transformed Arabidopsis thaliana through Agrobacterium mediated transformation, using binary vector pBI121, by floral dip method. phenotypic physiological parameters...
In the intestine, interaction between epithelial cells and macrophages (MΦs) create a unique immunoregulatory microenvironment necessary to maintain local immune tissue homeostasis. Human intestinal (IECs) have been shown express interleukin (IL)-10, which keeps integrity. We demonstrated that bacterial signaling through Toll-like receptor (TLR) 4 induces 15-deoxy-Δ-12,14-prostaglandin J2 (15d-PGJ2) synthesis in MΦs by cyclooxygenase (Cox)-2 expression. Here, we show TLR4...
Abstract Sterol glycosyltransferases ( SGT s) catalyse transfer of glycon moiety to sterols and their related compounds produce diverse glyco-conjugates or steryl glycosides with different biological pharmacological activities. Functional studies SGTs from Withania somnifera indicated role in abiotic stresses but details about under biotic stress are still unknown. Here, we have elucidated the function by silencing SGTL1 , SGTL2 SGTL4 . Down-regulation artificial miRNAs led enhanced...
Sterol glycosyltransferases regulate the properties of sterols by catalyzing transfer carbohydrate molecules to sterol moiety for synthesis steryl glycosides and acyl glycosides. We have analyzed functional role TTG15/UGT80B1 gene Arabidopsis thaliana in freeze/thaw heat shock stress using T-DNA insertional sgt knockout mutants. Quantitative study spatial as well temporal expression showed tissue-specific dynamic patterns throughout growth stages. Comparative responses Col-0, mutant...
Abstract Target of rapamycin (TOR) functions as a central sensory hub linking wide range external stimuli to gene expression. The mechanisms underlying stimulus-specific transcriptional reprogramming by TOR remain elusive. Here, we describe an in silico analysis Arabidopsis demonstrating that TOR-repressed genes are associated with either bistable or silent chromatin states. Both states regulated the signaling pathway high level histone H3K27 trimethylation (H3K27me3) deposited CURLY LEAF...
Background Childhood malnutrition remains a global concern, especially in low- and middle-income countries, is known to create an intergenerational cycle of illness poverty. Women's empowerment has gained recognition as potential catalyst for improving child nutrition. The objective this research was investigate the association between women's factors nutritional status children under five years age. Methods study used data from National Family Health Survey-5, conducted India 2019 2021 by...
Heat is a major environmental stress factor that confines growth, productivity, and metabolism of plants. Plants respond to such unfavorable conditions through changes in their physiological, biochemical developmental processes. Withania somnifera, an important medicinal plant, grows hot dry conditions, however, molecular mechanisms related adaptive properties are not known. Here, we elucidated members the sterol glycosyltransferases (SGT) gene family play roles survival W. somnifera under...
Stimulation of gastric parietal cells results in exocytic recruitment the proton pump (H+,K+-ATPase) from a pool intracellular membranes (tubulovesicles) to apical plasma membrane. We have previously reconstituted step this process, homotypic fusion tubulovesicles, and shown that they also fuse with liposomes protein-dependent manner [Duman, J. G., Singh, Lee, G. Y., Machen, T. E., Forte, (2002) Traffic 3, 203−17]. Further, lipid composition affects their ability undergo tubulovesicles. In...
Exocytic activation of gastric parietal cells represents a massive transformation. We studied step in this process, homotypic fusion H,K‐ATPase‐containing tubulovesicles, using R18 dequenching. Ca 2+ and Mg /ATP each caused dramatic dequenching, reflecting change distribution from 5% to 65–90% the assay's membranes 2.5 min. These stimuli also triggered between tubulovesicles liposomes. Independent confirmation that dequenching represented membrane was established by separating...
The maintenance of genetic information is important in eukaryotes notably through mechanisms occurring at the nuclear periphery where inner membrane proteins and pore-associated components are key factors regulating DNA damage response (DDR). However, this aspect DDR regulation still poorly documented plants. We addressed here how genomic stability impaired gamma-tubulin complex component 3-interacting protein ( gip1gip2 ) double mutants showing defective shaping. Using neutral comet assays...
This paper analyses the perception of public and private hospital departmental heads chief executives towards management issues compare their perceptions. A survey was conducted on staff members. total 200 members formed sample which 100 belonged to hospitals hospitals. The data were analysed with help t test analysis explanation responses respondents. study reveals that there is no significant difference in perceptions issues. It also revealed though difference, yet scores over Keywords:...