Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) intracellular polysaccharide (IPS) production of the G.lucidum optimised based on initial pH, starting glucose concentration agitation rate parameters response surface methodology (RSM). Molecularly, G. strain QRS 5120 generated 637 base pairs, which commensurate with related species. RSM, by...

Medicinal plants have been used in traditional medicine for centuries due to their phytochemical components and medicinal properties. In this study, wild herbs Oldenlandia corymbosa (Lidah Ular), Striga asiatica (Jarum Mas) Phyllanthus niruri (Dukung Anak) were collected from Universiti Sains Islam Malaysia, dried, grounded. The powdered samples extracted using hot water, ethyl acetate ethanol 48 hours. Phytochemical analysis revealed antioxidants such as flavonoids phenolics all extracts,...

In a commercial oyster mushroom farm, fruiting body base (FBB) was not harvested compared to the common cap and stem of parts, thus remained as waste. present study, unused FBB powdered subjected proximate analysis floured (FFBB). FFBB found contain 71.2% carbohydrate, 8.93% moisture, 7.18% fibre, 5.72% ash, 5.57% protein, 1.4% fat, while raw-FBB (RFBB) contained 7.57% 84.4% 5.17% 1.54% 0.85% fat. The high carbohydrate content hot-water extraction yielded 7.40 g polysaccharide (FFBBP). Total...

The depletion of fossil fuels and the emission greenhouse gases have increased demand for new sustainable energy sources, leading to growing interest in using fast-growing filamentous fungi as a source bioenergy. This study aimed optimize production exopolysaccharides (EPS) mycelial biomass (MB) from native medicinal mushroom, Lignosus rhinocerus, through submerged liquid fermentation. Using response surface methodology (RSM), it was found that glucose concentration speed agitation...

The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass production of exopolysaccharide (EPS) Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) applied a polynomial regression model with quadratic term used analyse the experimental data analysis variance (ANOVA). ANOVA showed that very significant (p are as follows: sucrose concentration 114.61 g/L, 1.62 g/L 5.81;...

The pellet morphology and diameter range (DR) of <i>Ganoderma lucidum</i> were observed in a repeated-batch fermentation (RBF) for the trio total production biomass, exopolysaccharide (EPS) endopolysaccharide (ENS). Two factors involved RBF; broth replacement ratio (BRR: 60%, 75% 90%) time point (BRTP: log, transition stationary phase) days. In RBF, 34.31 g/L biomass favoured small-compact pellets with DR 20.67 µm&lt; d &lt; 24.00 µm (75% BRR, day 11 BRTP). EPS 4.34 was prone to...

Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB designed to eliminate the wall growth and clumping mycelium in STRs. Ganoderma lucidum used as model its biomass exopolysaccharide (EPS) production were maximised by optimising agitation rate, glucose concentration, initial pH, aeration via response surface methodology (RSM). system generated 7.8 g/L...

Various crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan carbohydrate compound analysis. Crude extract from ethanol, cold hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, flavonoid content compositions. The activity the determined by 2,2' diphenyl-1-picrylhydrazyl (DPPH) scavenging method assay....

The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. central composite design was applied study significant interactions between chosen factors, if present. Design Expert software generated 20 runs. optimized conditions obtained as follows: rate 129.8 rpm, temperature at 27.8&degC, 6.06. tripled range 980 - 1040 mg/litre/day compared 310 mg/litre/day. From...

06Apr 2018 INCORPORATION OF PALM OIL FOR THE ENHANCEMENT BIOMASS AND POLYSACCHARIDES PRODUCTION THROUGH SUBMERGED FERMENTATION FROM LOCALLY ISOLATED PLEUROTUS SP. MYCELIUM. Rahayu Ahmad , Ingabire Ndagije Clementine Ju Sin Er S.N.A.B. Syed-Mustaffa and Wan Abd Al Qadr Wan-Mohtar. Department of Biotechnology, School Science Engineering, Manipal International University (MIU), 71800 Putra Nilai, Negeri Sembilan, Malaysia. Chemical Institute Biological Sciences, Faculty Science, Malaya, Kuala...

Medicinal plants have been used in traditional medicine for centuries due to their phytochemical components and medicinal properties. In this study, wild herbs Oldenlandia corymbosa (Lidah Ular), Striga asiatica (Jarum Mas) Phyllanthus niruri (Dukung Anak) were collected from Universiti Sains Islam Malaysia, dried, grounded. The powdered samples extracted using hot water, ethyl acetate ethanol 48 hours. Phytochemical analysis revealed antioxidants such as flavonoids phenolics all extracts,...

Kashmir falls in the great north-western complex of Himalayan ranges, at 34o North and 76o East with an average altitude 1850 metres amsl. Indigenous cattle constitute 51% total population State. The evaluation some important blood parameters indigenous for assessment animal health status as well course variation these animals was undertaken during summer to autumn, 2014. Twenty two from different pockets valley were studied haematobiochemical anti-oxidative parameters. values recorded Hb,...

This study investigates the bioactive compounds present in genus Pleurotus sp. mushrooms, with a focus on their antioxidant properties. Hot water extraction of mycelium was performed at three temperatures: 30°C, 65°C, and 100°C. A total 57.48 g biomass obtained through submerged fermentation basal medium. The objective to identify specific phytochemicals evaluate percentage free radical inhibition. initially cultured Potato Dextrose Agar plates later subjected fermentation. Six phytochemical...

This study investigates the bioactive compounds present in genus Pleurotus sp. mushrooms, with a focus on their antioxidant properties. Hot water extraction of mycelium was performed at three temperatures: 30°C, 65°C, and 100°C. A total 57.48 g biomass obtained through submerged fermentation basal medium. The objective to identify specific phytochemicals evaluate percentage free radical inhibition. initially cultured Potato Dextrose Agar plates later subjected fermentation. Six phytochemical...

Abstract The degradation of anthraquinone dye Reactive Blue 19 by using O 3 and / S 2 8 2- in the advanced oxidation processes is studied to investigate performance these two systems. response surface method with a Box-behnken Design was successfully applied identify relationship between operating variables such as initial concentration, dosage contact time order determine optimum condition. quadratic model for percentage COD removal (response) proved be significant dye. efficiency under...