A5011345774- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Molecular Biology Techniques and Applications
- Research on Leishmaniasis Studies
- Enzyme Catalysis and Immobilization
- Insect Pest Control Strategies
- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Plant biochemistry and biosynthesis
- Biosensors and Analytical Detection
- Aquaculture disease management and microbiota
- Toxoplasma gondii Research Studies
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Phytase and its Applications
- Malaria Research and Control
- Bacterial Genetics and Biotechnology
- biodegradable polymer synthesis and properties
- Influenza Virus Research Studies
- Microbial Metabolites in Food Biotechnology
National Institutes of Biotechnology Malaysia
2017-2025
Malaysia Genome Institute
2019-2023
Ministry of Science, Technology and Innovation
2016-2019
Universiti Sains Malaysia
2019
Universiti Putra Malaysia
2011-2012
Lignocellulose constitutes a major component of discarded wastes from various industries viz. agriculture, forestry and municipal waste treatment. The potential use lignocellulose such types biomass can be maximized by enzymatic degradation using glycoside hydrolases (GHs) oxidative enzymes to produce renewable fuels. Nonetheless, besides the slow rate low yields, is also physicochemically recalcitrant costly process, further limiting its mass utilization. Therefore, bioprospecting for...
The mesocarp of Elaeis guineensis is blessed with an abundance oil proven to be graced by the presence naturally occurring diverse family lipase, many which have yet studied. These lipases influenced innate quality while also being responsible for other biochemical reactions in fruit. To accurately determine capabilities these lipase species, single protein isolation was necessary. PLA1-3; a potentially important palm selected preliminary expression and purification facilitate later...
A series of new imidazole-phenazine derivatives were synthesized via a two-step process. The condensation 2,3-diaminophenazine and benzaldehyde proceeds with intermediate formation an aniline Schiff base, which undergoes subsequent cyclodehydrogenation in situ. structures the compounds characterized by 1D 2D NMR, FTIR HRMS. total thirteen imidazole phenazine validated for their inhibitory activity as anti-dengue agents vitro DENV2 NS2B-NS3 protease assay using fluorogenic Boc-Gly-Arg-Arg-AMC...
The chemical route of producing geranyl propionate involves the use toxic chemicals, liberation unwanted by-products as well problematic separation process. In view such problems, Rhizomucor miehei lipase (RML) covalently bound onto activated chitosan–graphene oxide (RML-CS/GO) support is suggested. Following analyses using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission and thermogravimetry, properties RML-CS/GO were characterized. A...
For decades, a Pap smear test has been applied as conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it method. Current biosensor such Hybrid (II) Capture resulted higher time consumption and cost. s Meanwhile, this study we provided facile, mini, rapid, highly sensitive, eco-friendly, cost-effective sensing system focusing on HPV strain 58 (HPV58) nano-size lab-on-chip technology genosensor. 30-mer of virus...
PpCHS is a member of the type III polyketide synthase family and catalyses synthesis flavonoid precursor naringenin chalcone from p-coumaroyl-CoA. Recent research reports production pyrone derivatives using either hexanoyl-CoA or butyryl-CoA as starter molecule. The Cys-His-Asn catalytic triad found in other plant predicted polypeptides conserved PpCHS. Site directed mutagenesis involving these amino acids residing active-site cavity revealed that volume plays significant role selection...
The rise in dengue cases tropical and sub-tropical areas has become a significant health concern. At present, there is no definitive cure for fever, which underscores the importance of identifying potent inhibitors. Dengue NS2B-NS3 protease prime drug target due to its vital function replication. Quercetin, flavone, anti-dengue virus properties but limited by low bioavailability. Previous studies have shown that methoxy substitution flavones improves bioavailability metabolic stability....
Dengue virus (DENV) is a globally significant human pathogen that annually infects more than ∼300 million of the Earth's population. While clinically approved anti-dengue vaccine, Dengvaxia available for treating such infection, its treatment limited to those infected with DENV serotypes 3 and 4 only. However, vaccine no longer treat dengue infections following suspended use after several discoveries on side effects humans. In view this recent development, collection plant extracts in...
A halophilic marine bacterial strain, PS-C1, was isolated from Sekinchan beach in Selangor, Malaysia. The 16S rRNA gene sequence analysis indicated that strain PS-C1 associated with the genus
Toxoplasma gondii is the etiologic agent of toxoplasmosis, a disease which can lead to morbidity and mortality fetus immunocompromised individuals. Due limited effectiveness or side effects existing drugs, search for better drug candidates still ongoing. In this study, we performed structure-based screening potential dual-targets inhibitors active sites T. targets such as uracil phosphoribosyltransferase (UPRTase) adenosine kinase (AK). First virtual compounds from National Cancer Institute...
An oligonucleotide DNA probe has been developed for the application in electrochemical biosensor early diagnosis of coronavirus disease (COVID-19). Here, virus microRNA from N-gene severe acute respiratory syndrome-2 (SARS-CoV-2) was used first time as a specific target detecting and became framework developing complementary probe. The sequence analysis carried out using bioinformatics tools including basic local alignment search tools, multiple CLUSTLW, database (miRbase), database, gene...
The widespread use of herbicides that may contain the recalcitrant halogenated compound 3-chloropropionic acid poses significant environmental hazards and prove detrimental to human kind.Therefore, it is important an environmentally friendly bio-based method detoxify such substance developed.Consequently, research detailed here investigated isolation identification a bacterial strain could degrade (3CP) as its sole carbon source.A dehalogenase producing bacteria capable utilizing 3CP was...
AbstractDengue virus (DENV) infection remains a formidable public health concern, causing significant fatalities worldwide. The unavailability of specific anti-DENV therapeutics and poor response towards dengue vaccine have impeded efforts to prevent control this tropical disease, thereby, there is pressing need for effective alternative therapeutics. Curcuma species including aeruginosa long history traditional use the prevention treatment various ailments, viral infections. While it...
ABSTRACT Vibrio sp. PBL-C16 is a bacterium that was isolated from Batu Laut Beach in Selangor, Malaysia. Here, we present high-quality annotated draft genome of strain and suggest its potential glycoside hydrolase enzymes for polysaccharide degradation.
Abstract α-Amylase plays a crucial role in the industrial degradation of starch. The genus Jeotgalibacillus underexplored marine bacteria family Caryophanaceae has not been investigated terms α-amylase production. Herein, we report comprehensive analysis an (AmyJM) from malaysiensis D5 T (= DSM28777 = KCTC33550 ). Protein phylogenetic indicated that AmyJM belongs to glycoside hydrolase 13 subfamily 5 (GH13_5) and exhibits low sequence identity with known α-amylases, its closest counterpart...
New advancements in developing sensitive and selective biosensors have demonstrated outstanding potential for Deoxyribonucleic Acid (DNA biosensors). The detection mode of DNA primary depends on a particular hybridization that precisely occurs the surface physical transducer can only be detected using high-performance assays due to slight current changes. analytical performance (sensitivity) biosensor is conclusively rely confluence constructing sensing surface, which must optimized. Thus,...
Aims: This study aims to describe the biochemical and kinetic properties of a dehalogenase produced by bacterium, Bacillus cereus WH2 (KU721999), that is uniquely adept at degrading β-haloalkanoic acid, i.e., 3-chloropropionic acid (3-CP), using it as bacterium's sole carbon source.The bacterium was isolated from abandoned agricultural land in Universiti Teknologi Malaysia previously exposed herbicides pesticides.Methodology results: The B. impressively removed 97% 3-CP after 36 h...
Roseovarius sp. strain PS-C2 is a bacterium that was isolated from Sekinchan Beach in Selangor, Malaysia, using an ex situ cultivation technique. Here, we present high-quality annotated draft genome of and suggest potential applications this bacterium.
The E6 region has higher protuberant probability annealing than consensus probe focusing on another in the human papillomavirus (HPV) genome terms of detection and screening method. Here, we designed first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for detections an early phase cervical cancer became a fundamental evolution electrochemical HPV biosensor. Gene profiling ssDNA sequences been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment...