A5035892565- Enzyme Structure and Function
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Traditional and Medicinal Uses of Annonaceae
- Wood and Agarwood Research
- Bioactive Compounds and Antitumor Agents
- Medicinal Plants and Neuroprotection
- Bacterial Genetics and Biotechnology
- Natural product bioactivities and synthesis
- Identification and Quantification in Food
- Ion channel regulation and function
- Morinda citrifolia extract uses
- Bacteriophages and microbial interactions
- Phytochemical compounds biological activities
- Cocoa and Sweet Potato Agronomy
- Plant pathogens and resistance mechanisms
- Natural Antidiabetic Agents Studies
- Calcium Carbonate Crystallization and Inhibition
- Food Allergy and Anaphylaxis Research
- Bacterial biofilms and quorum sensing
- Biofuel production and bioconversion
- Gout, Hyperuricemia, Uric Acid
- Phytochemistry and biological activity of medicinal plants
- Tea Polyphenols and Effects
- Computational Drug Discovery Methods
Sultan Idris Education University
2014-2025
Kyoto University
2017
Universiti Putra Malaysia
2010-2013
The mesocarp of Elaeis guineensis is blessed with an abundance oil proven to be graced by the presence naturally occurring diverse family lipase, many which have yet studied. These lipases influenced innate quality while also being responsible for other biochemical reactions in fruit. To accurately determine capabilities these lipase species, single protein isolation was necessary. PLA1-3; a potentially important palm selected preliminary expression and purification facilitate later...
Significance Statement TRPC6 is a receptor-activated nonselective cation channel. Naturally occurring mutations in this channel are associated with FSGS. FSGS-associated appear to have “gain-of-function” mechanism, but the exact mechanism remains unclear. The authors show that negative feedback regulation induced by cellular calcium levels, called Ca 2+ -dependent inactivation (CDI), impaired mutations. Dysfunctional coiled-coil assembly mutated disrupts calmodulin bridging which essential...
Xanthine oxidase is an enzyme responsible for the build-up of excess uric acid that causes gout, one most common inflammatory arthritis found in Malaysia. The present study investigate silico xanthine inhibitory activities alkaloids isolated from Alphonsea cylindrica and elliptica. Serial column chromatography led to isolation compounds which were characterized as kinabaline, atherospermidine, cyathocaline, N-methylouregidione on basis various spectroscopic techniques. Binding orientation...
Aminoanthraquinones were successfully synthesized via two reaction steps. 1,4-Dihydroxyanthraquinone (1) was first subjected to methylation, reduction and acylation give an excellent yield of anthracene-1,4-dione (3), 1,4-dimethoxyanthracene-9,10-dione (5) 9,10-dioxo-9,10-dihydroanthracene-1,4-diyl diacetate (7). Treatment 1, 3, 5 7 with BuNH2 in the presence PhI(OAc)2 as catalyst produced seven aminoanthraquinone derivatives 1a, b, 3a, 5a–d. Amination 3 afforded three new...
PT7 (A1/O4/O3); repression; over
Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with final yield 48% 25-fold increase in specific activity. The protein had exhibited first ever reported homodimer size 65 kDa SDS-PAGE MALDI-TOF, which is totally distinct that typically 33 monomer P. aeruginosa. organic solvent stability experiment demonstrated pattern completely opposed the rules laid...
The structural gene of elastase strain K (elastase from Pseudomonas aeruginosa K), namely HindIII1500PstI, was successfully sequenced to contain 1497 bp. amino acid sequence, deduced the nucleotide revealed that mature consists 301 acids, with a molecular mass 33.1 kDa, and contains conserved motif HEXXH, zinc ligands residues involved in catalysis K. cloned shuttle vector, pUCP19, transformed into Escherichia coli strains TOP10, KRX, JM109 Tuner™ pLacI as well P. PA01 (A.T.C.C. 47085) S5,...
A phytochemical study of Alphonsea cylindrica King (unreported) has led to the isolation six alkaloids. The compounds were identified as kinabaline (1; azafluorenone alkaloid), muniranine (2), O-methylmoschatoline (3; oxoaporphine lysicamine (4), atherospermidine (5) and N-methylouregidione (6; 4, 5-dioxoaporphine alkaloid). structures isolated determined based on spectroscopic techniques by comparison with data reported in literature. Alkaloid 2 was a new derivative while alkaloids 1, 3 – 6...
Obesity is one of the serious health condition in world and contributes to many chronic illnesses augmenting high risk death rate.With purpose obesity treatment, natural sources come be significant replace commercial drugs due their harmful effect body.Therefore, a lot studies were conducted on potential as medicinal herbs.Aquilaria sp.plants are well known traditional medicine sedative, analgesic digestive.Even though there no reports applications Aquilaria sp.towards this plant has...
The study aimed to isolate and identify the phytochemical components of Polyalthia lateriflora leaves evaluate antimicrobial activity. Six well-known compounds, including three triterpene lupeol (1) betulinic acid (2), β-Sitosterol-β-D-glucoside (3) oxoaporphine alkaloids O-methylmoschotaline (4), liriodenine (5) atherosperminine (6). Structural elucidation compounds were established through spectroscopic techniques such as 1D 2D NMR (1H 13C, DEPT, COSY, NOESY, HMBC, HMQC), IR LC-MS....
Obesity is one serious health condition that contributes to various chronic diseases. The inhibition of pancreatic lipase a promising treatment for obesity.The present study was designed investigate anti-porcine effect isolated compounds from Aquilaria subintegra and its mechanism.Compounds were with serial column chromatography their structure identified using spectroscopic methods. Isolated tested anti-lipase potential activity colorimetric assay. prediction energy binding between enzyme...
A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes species. The gene, which consisted 1440 bp nucleotides deduced 479 amino acid residues, successfully expressed in pGEX-4T-1 expression system the presence 1.0 mM IPTG, after incubation 6 h at 37 degrees C. Under these conditions, recombinant was, subsequently,...
Preservatives are usually added to food products ensure longer shelf life and prevent decomposition process microbial growth. However, synthetic preservatives can also give negative side effect health harmful human animal physiology. Based on the potential of herbs spices as antimicrobial agent, purpose this study is identify antibacterial activity from extracts some local spices: Phaeomeria speciosa (P. speciosa), Aquilaria subintegra (A. subintegra), Polygonum minus minus), Syzygium...
A new series of aminoanthraquinone were successfully synthesized via two step reaction.Firstly 1,4-(dihyroxy)anthracene-9,10-dione was treated with butylamine in the presence iodobenzene-diacetate to gives 2-(butylamino)-1,4dihydroxyanthraquinone (1) (90%).In second 1 subjected reduction, methylation and acylation.Reduction using NaBH 4 result 2-(butyamino)anthracene-1,4-dione (2) where as give a mixture 2-(butyamino)-1-hydroxy-4methoxyanthracene-9,10-dione (3a)...
Background:Pseudomonas protein expression in E. coli is known to be a setback due significant genetic variation and absence of several elements for regulation activation Pseudomonas proteins. Modifications promoter/repressor system shuttle plasmid maintenance have made the stable active possible both sp. coli. Objectives: Construction vectors overexpression proteins Materials Methods:Pseudomonas-Escherichia vectors, pCon2(3), pCon2(3)-Kan pCon2(3)-Zeo as well pCon4 pCon5 were constructed...
Protein expression by means of vector construction may facilitate in the synthesis protein sufficient amount for characterization, quorum sensing and molecular diagnostic studies. However, significant also can be expressed even under non-inducing repressed conditions when using many vectors available nowadays. In this communication, we report on design several genetic elements which resulted more regulable thus tightly repressible system grown conditions. To construct tight regulation...