Abstract The SKY hot spring is a unique site filled with thick layer of plant litter. With the advancement next-generation sequencing, it now possible to mine many new biocatalyst sequences. In this study, we aimed (i) identify metataxonomic prokaryotes and eukaryotes in microbial mats using 16S 18S rRNA markers, (ii) explore carbohydrate degrading enzymes (CAZymes) that have high potential for future applications. Green mat, predominantly photosynthetic bacteria, was attached submerged or...

Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-degrading grouped into pullulanases (types I type II) hydrolase I, II III). Generally, these hydrolyse the α-1,6 glucosidic bonds (and α-1,4 for certain enzyme groups) of substrates form reducing sugars such as glucose, maltose, maltotriose, panose or isopanose. This review covers two main aspects: (i) bibliometric analysis publications patents related to pullulan-degrading (ii) biological aspects free...

Abstract Background Hot spring biofilms provide a window into the survival strategies of microbial communities in extreme environments and offer potential for biotechnological applications. This study focused on green brown thriving submerged plant litter within Sungai Klah hot Malaysia, characterised by temperatures 58–74 °C. Using Illumina shotgun metagenomics Nanopore ligation sequencing, we investigated diversity functional metagenome-assembled genomes (MAGs) with specific focus biofilm...

A halo-thermophilic bacterium, Roseithermus sacchariphilus strain RA (previously known as Rhodothermaceae bacterium RA), was isolated from a hot spring in Langkawi, Malaysia. complete genome analysis showed that the harbors 57 glycoside hydrolases (GHs), including multi-domain xylanase (XynRA2). The full-length XynRA2 of 813 amino acids comprises family 4_9 carbohydrate-binding module (CBM4_9), 10 hydrolase catalytic domain (GH10), and C-terminal (CTD) for type IX secretion system (T9SS)....

A bacterial strain, designated RA, was isolated from water sample of a hot spring on Langkawi Island Malaysia using marine agar. Strain RA is an aerophilic and thermophilic microorganism that grows optimally at 50–60°C capable growing in broth containing 1–10% (w/v) NaCl. 16S rRNA gene sequence analysis demonstrated this strain most closely related (< 90% identity) to Rhodothermaceae, which currently comprises six genera: Rhodothermus (two species), Salinibacter (three Salisaeta (one...

Rhodothermaceae bacterium RA is a halo-thermophile isolated from saline hot spring. Previously, the genome of this was sequenced using HiSeq 2500 platform culminating in 91 contigs. In report, we report on resequencing its complete PacBio RSII platform. The has GC content 68.3%, 4,653,222 bp size, and encodes 3711 genes. We are interested understanding carbohydrate metabolic pathway, particular lignocellulosic biomass degradation pathway. Strain harbors 57 glycosyl hydrolase (GH) genes that...

Staphylococcus aureus is a bacterium that causes various diseases in humans. Cold plasma found to be an alternative eliminate S. aureus. Most studies of cold on mainly focus the physiochemical changes cells. So far, biological responses treatment under different durations have not yet been evaluated. In this study, results showed was effective eliminating At initial exposure (1 min), treated cells gene upregulations stress proteins, antioxidants, nitrosative stress, and transporter but no...

Generally, most of the setups plasma jet adopted rare gases (e.g., helium and argon) as working gas. The drawback using is that it cannot be continuously used gas tank volume limited, cost to yield also higher. Thus, a solely air could potentially promising solution. In this study, low-temperature only was developed. device optimized by adjusting control circuit (deadband, current, voltage duty ratio), frequencies (50-350 kHz), pump flow-rate (0-8 L/min), geometric size each component....

A halophilic marine bacterial strain, PS-C1, was isolated from Sekinchan beach in Selangor, Malaysia. The 16S rRNA gene sequence analysis indicated that strain PS-C1 associated with the genus

C old plasma is a disinfection technique widely used in food, agricultural, and medical industries. This work cold to sterilize Pseudomonas aeruginosa cell survivability was determined. RNA sequencing determine the bacterial responses at 1 minute (T1), 3 minutes (T3), 5 (T5) of treatments. The results show that longer treatment leads lower survivability. Cold induced rapid P. aeruginosa. Gene Ontology enrichment analysis showed T5 had most enriched terms compared T1 T3. affected genes were...

Zhouia amylolytica CL16 was isolated from the mangrove soil of Tanjung Piai, Malaysia. The present work reports draft genome sequence this bacterium. consists 113 glycoside hydrolases, 40 glycosyltransferases, 4 polysaccharide lyases, 23 carbohydrate esterases, 5 auxiliary activities, and 27 carbohydrate-binding modules, which warrant further investigation.

Nitrogen cycling, a critical biogeochemical process in ecosystems, involves complex microorganism network. In nitrification, ammonia oxidation is mainly governed by ammonia-oxidizing archaea (AOA) acidic soil. Limited information exists about these taxa tropical peatlands. This genome-centric metagenomic study aimed to identify key and their functional potential driving nitrification After cleaning Illumina reads, draft bins were created, refined, reassembled, decontaminated through various...

Introduction. Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as medicine treating various diseases, including infections caused by Candida albicans , an opportunistic pathogen that overgrow in individuals with weakened immune system. Gap Statement. To date, there has been less molecular study on cold plasma-treated C. . Research Aim. The aims to fill gap understanding response treatment. Methodology. This...

This data report presents prokaryotic metagenome-assembled genomes (MAGs) from a hot spring stream with temperatures between 64 and 100°C. The water was filtered the extracted total DNA sequenced using Illumina HiSeq 2500 platform. Approximately 80 Gb of raw were generated, which subsequently assembled MEGAHIT v1.2.9. MAGs generated MetaWRAP binning approaches MetaBAT2, CONCOCT MaxBin2. We constructed 25 medium-quality 24 high-quality archaeal MAGs, 152 112 bacterial MAGs. fasta files these...

Tropical peatlands significantly influence local and global carbon nitrogen cycles, yet they face growing pressure from anthropogenic activities. Land use changes, such as peatland forests conversion to oil palm plantations, affect the soil microbiome greenhouse gas (GHG) emissions. However, temporal dynamics of microbial community changes their role GHG indicators are not well understood. This study examines peat chemistry, communities, emissions 2016 2020 in a logged-over secondary swamp...

Roseovarius sp. strain PS-C2 is a bacterium that was isolated from Sekinchan Beach in Selangor, Malaysia, using an ex situ cultivation technique. Here, we present high-quality annotated draft genome of and suggest potential applications this bacterium.

The majority of the members in order Rhodothermales are underexplored prokaryotic extremophiles. Roseithermus, a new genus within Rhodothermales, was first described 2019. Roseithermus sacchariphilus is only species this genus. current report aims to evaluate transcriptomic responses R. strain RA when cultivated on beechwood xylan. Strain doubled its growth Marine Broth (MB) containing xylan compared alone. harbors 54 potential glycosyl hydrolases (GHs) that affiliated with 30 families,...

Cellulomonas sp. PS-H5 was isolated from Sekinchan Beach in Selangor, Malaysia, using an ex situ cultivation method. The present work reports a high-quality draft annotated genome sequence of this strain and suggests its potential glycoside hydrolase enzymes for cellulose, hemicellulose, starch degradations.