A5070607734- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Gene Regulatory Network Analysis
- Protein Structure and Dynamics
- Identification and Quantification in Food
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Prion Diseases and Protein Misfolding
- Bat Biology and Ecology Studies
- Computational Drug Discovery Methods
- Neonatal Health and Biochemistry
- Viral Infectious Diseases and Gene Expression in Insects
- Aquaculture disease management and microbiota
- Microbial metabolism and enzyme function
- Lepidoptera: Biology and Taxonomy
- Bioinformatics and Genomic Networks
- Species Distribution and Climate Change
- Analytical Chemistry and Chromatography
- Genetics, Bioinformatics, and Biomedical Research
- vaccines and immunoinformatics approaches
- Environmental DNA in Biodiversity Studies
University of Technology Malaysia
2016-2025
Tun Hussein Onn University of Malaysia
2019-2023
Newcastle University Medicine Malaysia
2019
Natural History Museum Aarhus
2017
Natural History Museum of Denmark
2017
National University of Malaysia
2011
Thomas More Universitas
2009
University of Exeter
2005
Malaysia has a great number of hot springs, especially along the flank Banjaran Titiwangsa mountain range. Biological studies Malaysian springs are rare because lack comprehensive information on their microbial communities. In this study, we report cultivation-independent census to describe communities in six springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Serai (SS), Semenyih (SE), and Ayer Hangat (AH) exhibit circumneutral pH with temperatures ranging from 43°C 90°C. Genomic...
Abstract The SKY hot spring is a unique site filled with thick layer of plant litter. With the advancement next-generation sequencing, it now possible to mine many new biocatalyst sequences. In this study, we aimed (i) identify metataxonomic prokaryotes and eukaryotes in microbial mats using 16S 18S rRNA markers, (ii) explore carbohydrate degrading enzymes (CAZymes) that have high potential for future applications. Green mat, predominantly photosynthetic bacteria, was attached submerged or...
In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to via fermentation. The crucial step in of from is saccharification. Many commercial cellulase enzyme cocktails, such as Spezyme(®) CP (Genencor), Acellerase™ 1000 Celluclast(®) 1.5L (Novozymes), are ineffectively release free glucose pretreated without additional β-glucosidase.In this study, for first time, a β-glucosidase DT-Bgl gene (1359 bp) was...
DNA polymerase β, a member of the X-family polymerases, undergoes complex regulations both in vitro and vivo through various posttranslational modifications, including phosphorylation methylation. The impact these modifications varies depending on specific amino acid undergoing alterations. In vitro, methylation β with enzyme protein arginine methyltransferase 6 (PRMT6) at R83 R152 enhances activity by improving binding processivity. Although studies have shown that improves binding,...
Abstract Southeast (SE) Asia is 1 of the most biodiverse regions in world, and it holds approximately 20% all mammal species. Despite this, majority SE Asia's genetic diversity still poorly characterized. The growing interest using environmental DNA to assess monitor Asian species, particular threatened mammals—has created urgent need expand available reference database mitochondrial barcode complete mitogenome sequences. We have partially addressed this by generating 72 new sequences...
Abstract Background Hot spring biofilms provide a window into the survival strategies of microbial communities in extreme environments and offer potential for biotechnological applications. This study focused on green brown thriving submerged plant litter within Sungai Klah hot Malaysia, characterised by temperatures 58–74 °C. Using Illumina shotgun metagenomics Nanopore ligation sequencing, we investigated diversity functional metagenome-assembled genomes (MAGs) with specific focus biofilm...
A new subfamily of glycosyl hydrolase family GH13 was recently proposed for α-amylases from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo, GtamyII), Bacillus aquimaris (BaqA), 95 other putative protein homologues. To understand this subfamily, we report crystal structures truncated ASKA (TASKA). is a thermostable enzyme capable producing high levels maltose. Unlike GTA, biochemical analysis showed that Ca(2+) ion supplementation enhances the catalytic...
A halo-thermophilic bacterium, Roseithermus sacchariphilus strain RA (previously known as Rhodothermaceae bacterium RA), was isolated from a hot spring in Langkawi, Malaysia. complete genome analysis showed that the harbors 57 glycoside hydrolases (GHs), including multi-domain xylanase (XynRA2). The full-length XynRA2 of 813 amino acids comprises family 4_9 carbohydrate-binding module (CBM4_9), 10 hydrolase catalytic domain (GH10), and C-terminal (CTD) for type IX secretion system (T9SS)....
Population surveys and species recognition for roosting bats are either based on capture, sight or optical-mechanical count methods. However, these methods intrusive, tedious and, at best, provide only statistical estimations. Here, we demonstrated the successful use of a terrestrial Light Detection Ranging (LIDAR) laser scanner remotely identifying determining exact population in caves. LIDAR accurately captured 3D features their spatial distribution patterns minimal light. The...
Abstract Fatal familial insomnia (FFI) and Creutzfeldt‐Jakob disease (CJD) are associated to the same mutation at codon 178 but differentiate into clinicopathologically distinct diseases determined by this a naturally occurring methionine–valine polymorphism 129 of prion protein gene. It has been suggested that clinical pathological difference between FFI CJD is caused different conformations protein. Using molecular dynamics (MD), we investigated effect on conformation normal elevated...
The spread of infectious illnesses has been a significant factor restricting aquaculture production. To maximise aquatic animal health, vaccination tactics are very successful and cost-efficient for protecting fish animals against many disease pathogens. However, due to the increasing number immunological cases their complexity, it is impossible manage, analyse, visualise, interpret such data without assistance advanced computational techniques. Hence, use immunoinformatics tools crucial, as...
Many environmental pollutions are caused by the abundance of xenobiotic compounds in nature. For instance, halogenated released from chemical industries were proven to be toxic and recalcitrant environment. However, haloalkanoic acid dehalogenases can catalyse removal halides organic haloacids thus have gained interest for bioremediation synthesis industrial chemicals. This study presents first structural model key residues non-stereospecific dehalogenase, DehE, Rhizobium sp. RC1. The enzyme...
Eurycoma longifolia Jack (Simaroubaceae) commonly known as Tongkat Ali is one of the most important plants in Malaysia. The plant extracts (particularly roots) are widely used for treatment cough and fever besides having antimalarial, antidiabetic, anticancer aphrodisiac activities.This study assesses extent adulteration E. herbal medicinal products (HMPs) using DNA barcoding validated by HPLC analysis.Chloroplastic rbcL nuclear ITS2 barcode regions were present study. sequences generated...
A bacterial strain, designated RA, was isolated from water sample of a hot spring on Langkawi Island Malaysia using marine agar. Strain RA is an aerophilic and thermophilic microorganism that grows optimally at 50–60°C capable growing in broth containing 1–10% (w/v) NaCl. 16S rRNA gene sequence analysis demonstrated this strain most closely related (< 90% identity) to Rhodothermaceae, which currently comprises six genera: Rhodothermus (two species), Salinibacter (three Salisaeta (one...