A5077759407- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Probiotics and Fermented Foods
- Plant pathogens and resistance mechanisms
- Oral microbiology and periodontitis research
- Plant Pathogens and Fungal Diseases
- Infections and bacterial resistance
- Identification and Quantification in Food
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Insect symbiosis and bacterial influences
- Congenital heart defects research
- Diatoms and Algae Research
- Biosensors and Analytical Detection
- Cassava research and cyanide
- Bacillus and Francisella bacterial research
- Molecular Biology Techniques and Applications
- Parasites and Host Interactions
- Microbial Metabolism and Applications
University of Malaya
2014-2020
Malaysia has a great number of hot springs, especially along the flank Banjaran Titiwangsa mountain range. Biological studies Malaysian springs are rare because lack comprehensive information on their microbial communities. In this study, we report cultivation-independent census to describe communities in six springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Serai (SS), Semenyih (SE), and Ayer Hangat (AH) exhibit circumneutral pH with temperatures ranging from 43°C 90°C. Genomic...
Abstract Jeotgalibacillus malaysiensis , a moderate halophilic bacterium isolated from pelagic area, can endure higher concentrations of sodium chloride (NaCl) than other type strains. In this study, we therefore chose to sequence and assemble the entire J. genome. This is first report provide detailed analysis genomic features perform genetic comparisons between microorganism halophiles. encodes native megaplasmid (pJeoMA), which greater 600 kilobases in size, that absent sequenced species...
Schistosomiasis is a neglected tropical disease that affects more than 200 million people worldwide. The main disease-causing agents, Schistosoma japonicum, S. mansoni and haematobium, are blood flukes have complex life cycles involving snail intermediate host. In Asia, japonicum causes hepatointestinal (schistosomiasis japonica) challenging to control due broad distribution of its hosts range animal reservoir hosts. China, extensive efforts been underway this parasite, but genetic...
Quorum sensing is a well-studied cell-to-cell communication method that involves cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, bacterium isolated from plant material compost pile was found to possess quorum activity based on bioassay screening. Isolate YL12 identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing rpoD gene which the isolate as Aeromonas caviae. High...
Phylogenetic and taxonomic characterization was performed for bacterium RB-25T, which isolated from a soil sample collected in former municipal landfill site Puchong, Malaysia. Growth occurred at 20–37 °C pH 5–8 but not the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C16:0, C18:1ω7c summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). Ubiquinone-8 only isoprenoid quinone detected. Polar lipid analysis revealed phospholipid, phosphoaminolipid, phosphatidylethanolamine,...
A Gram-stain-positive, endospore-forming, rod-shaped bacterial strain, designated D5 T , was isolated from seawater collected a sandy beach in southern state of Malaysia and subjected to polyphasic taxonomic study. Sequence analysis the 16S rRNA gene demonstrated that this isolate belongs genus Jeotgalibacillus with 99.87 % similarity alimentarius JCM 10872 . DNA–DNA hybridization strain J. 26.3 relatedness. The peptidoglycan type A1α linked directly l -lysine as diamino acid. predominant...
A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism isolated from soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using quorum-quenching enrichment medium. Growth occurred at 4-30 °C, pH 6-11 and moderately high salinity (0-15 %, w/v, NaCl), with optimal growth 26 7-8 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that L10.15T...
A bacterial strain, designated RA, was isolated from water sample of a hot spring on Langkawi Island Malaysia using marine agar. Strain RA is an aerophilic and thermophilic microorganism that grows optimally at 50–60°C capable growing in broth containing 1–10% (w/v) NaCl. 16S rRNA gene sequence analysis demonstrated this strain most closely related (< 90% identity) to Rhodothermaceae, which currently comprises six genera: Rhodothermus (two species), Salinibacter (three Salisaeta (one...
Abstract Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found various habitats Antarctica. Quorum quenching (QQ) disruption of cell-to-cell communication (known as quorum sensing), which has previously been described mesophilic bacteria. This study demonstrated QQ activity psychrotolerant strain, versutus strain L10.15 T , isolated from soil sample obtained near an elephant seal wallow Whole genome analysis this revealed presence N -acyl...
Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered consecutive sputum collected a CF patient identified as pnomenusa underwent polyphasic taxonomic analysis. The were found to be Gram-negative, facultative anaerobic motile bacilli subsequently designated strains 6399T (=LMG29626T=DSM103228T) 7641...
Abstract Arthrobacter alpinus R3.8 is a psychrotolerant bacterial strain isolated from soil sample obtained at Rothera Point, Adelaide Island, close to the Antarctic Peninsula. Strain was sequenced in order help discover potential cold active enzymes with biotechnological applications. Genome analysis identified various adaptation genes including some coding for anti-freeze proteins and cold-shock proteins, involved bioremediation of xenobiotic compounds naphthalene, chitinolytic N...
Quorum sensing is a unique bacterial communication system which permits bacteria to synchronize their behaviour in accordance with the population density. The operation of this network involves use diffusible autoinducer molecules, termed N-acylhomoserine lactones (AHLs). Serratia spp. are well known for quorum regulate expression various genes. In study, we aimed characterized AHL production bacterium designated as strain RB-25 isolated from former domestic waste landfill site. It was...
Pandoraea is an emerging respiratory pathogen capable of causing chronic lung infections in people with cystic fibrosis (CF), but the clinical significance this infection ambiguous. We have sequenced and annotated genomes two multidrug-resistant pnomenusa isolates recovered 11 months apart from same CF patient.
We report the first complete genome sequence of Pandoraea sp. strain RB-44, which was found to possess quorum-sensing properties. To best our knowledge, this is documentation both a and properties species.
ABSTRACT In this report, we announce the complete genome sequence of Aeromonas hydrophila strain YL17. Single-molecule real-time (SMRT) DNA sequencing was used to generate and genome-wide methylation profile environmental isolate. A total five unique methyltransferase recognition motifs were reported here.
A Gram-stain-negative, aerobic, rod-shaped, leaf-associated bacterium, designated JS23T, was isolated from surface-sterilized leaf tissue of an oil palm grown in Singapore and investigated by polyphasic taxonomy. Phylogenetic analyses based on 16S rRNA gene sequences 180 conserved genes the genome several members Burkholderiaceae revealed that strain JS23T formed a distinct evolutionary lineage independent other taxa within family Burkholderiaceae. The predominant ubiquinone Q-8. primary...
DATA REPORT article Front. Microbiol., 08 February 2016Sec. Antimicrobials, Resistance and Chemotherapy Volume 7 - 2016 | https://doi.org/10.3389/fmicb.2016.00109
In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair cognate luxI/R homologs was identified where luxI homolog, ppnI, found adjacent to a luxR ppnR1. An additional orphan ppnR2, also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene distinct from those nearest neighbor viz. Burkholderia spp....