We have determined the consequences of insulin-like growth factor-binding protein-1 (IGFBP-1) administration alone and in combination with factor-I (IGF-I). Human recombinant IGF-I, infused as a bolus into male Wistar rats, induced fall plasma glucose to 72 +/- 3% baseline 15 min after injection. Co-infusion equimolar concentrations human IGFBP-1 abolished IGF-I-induced (P less than 0.001). Injection caused rise levels 0.002). The half life IGFBP-1, measured using primate-specific RIA, was...

Transgenic mice that constitutively overexpressed rat insulin-like growth factor binding protein-1 (IGFBP-1) were generated to determine the effects of overexpression IGFBP-1 on and development. In offspring three founders showed high levels expression, birth weight was significantly reduced approximately 83-92% their nontransgenic littermates. The transgenic gained less 3.5-8 g lighter than littermates at 40 days age. difference in body between wild-type most apparent around time weaning...

Cultured human fetal liver explants were used to study regulation of the GH-independent insulin-like growth factor-binding protein (BP-28). BP-28 was produced by at approximately 200 ng/mg total tissue protein.day between days 3 and 6 in culture inhibited 60% after 3-day exposure cycloheximide (10 micrograms/mL). production stimulated over 3-fold 20 mmol/L theophylline, 2-fold 30 mumol/L forskolin, 90% 10 micrograms/mL glucagon. The glucose concentration medium inversely regulated...

Low levels of IGF-binding protein 1 (IGFBP1) are associated with metabolic syndrome and predict diabetes development in men. The aim this study was to determine the IGFBP1 women who later develop diabetes, relation abdominal obesity, compare these those men.IGFBP1 were determined at baseline after 8 years a case-control, prospective Swedish aged 35-56 years. Individuals normal oral glucose tolerance test (OGTT) developed abnormal regulation (n=240) pair matched controls for age family...

The liver is the main source and insulin regulator of IGF binding protein 1 (IGFBP-1) in humans. Here we examined how serum IGFBP-1 concentrations are related to directly measured hepatic sensitivity fat content humans.We fasting (fS) by proton magnetic resonance spectroscopy 113 nondiabetic subjects. In addition, was using euglycemic hyperinsulinemic clamp (insulin 0.3 mU/kg.min) technique combination with infusion [3-(3)H]glucose 78 subjects.fS-IGFBP-1 were inversely (r = -0.38, P <...

Abstract Background/Objectives Insulin-like growth factor-binding protein (IGFBP)-1 is a marker of insulin resistance. Lower IGFBP-1 associated with increased adiposity. The aims this study were to determine whether and its ligand, IGF-I, are weight waist measurements across mid-life old age, predict survival. Subjects/Methods Swedish Adoption/Twin Study Aging (SATSA) includes extensive in-person testing same-sex twins over 30-year period. dataset for which baseline fasting ( n = 512; 251...

Insulin-like growth factor-binding protein-3 (IGFBP-3), after first associating with IGF-I or IGF-II, is able to associate the acid-labile subunit (ALS) and form a 140-kDa complex. To investigate factors regulating ternary complex formation in vivo, human (h) IGFBP-3, hIGF-I, hALS were administered various combinations GH-deficient (dw/dw) rats. hIGFBP-3 had pattern of disappearance from rat circulation, an initial phase lasting minutes prolonged phase(s) hours. If coinjected significantly...

Cultured human fetal hepatic explants were used to study the role of hexose uptake in regulating production GH-independent insulin-like growth factor-binding protein (IGFBP-1). Cytochalasin B (100 μM), but not cytochalasin E, inhibited 2-deoxyglucose by liver and increased IGFBP-1 2-fold. Two agents which stimulate cyclic nucleotide-dependent pathways, cholera toxin theophylline, also this system both stimulated production. When added a maximally inhibiting concentration (250 was further...

Insulin-like growth factor-binding protein-3 (IGFBP-3) forms a 140-kilodalton (kDa) complex with an acid-labile glycoprotein after complexing IGF-I or IGF-II. To investigate the bioavailability of circulating IGFs in rat, we determined molecular distribution and disappearance iv bolus human IGFBP-3 (130 micrograms/kg). Serum fractions were assayed size-separation chromatography for (h) rat (r) specific RIAs. Within 2 min injection, 52.9 +/- 1.6% hIGFBP-3 appeared 140-kDa complex. This form...

<b>PURPOSE</b> We investigated the 10-year mortality rates in a trial that tested case-based intervention primary care aimed at reducing gap between evidence-based goals and clinical practice patients with coronary heart disease (CHD). <b>METHODS</b> A prospective randomized controlled pragmatic was undertaken setting. New guidelines, intensified lipid-lowering recommendations CHD, were mailed to all general practitioners region presented lecture 1995. General (n = 54) CHD 88) assigned...

We conducted a double-blind crossover study to determine which patient characteristics best predict beneficial response combined insulin-glyburide therapy. Glyburide (15 mg/day) or placebo was added the treatment regimen of 31 insulin-treated type II (non-insulin-dependent) diabetic subjects. During glyburide therapy, there significant improvement in glycemic control with reduction glycosylated hemoglobin from 9.9 ± 1.3 9.1 1.3% (P &amp;lt; .001). Patients who responded had higher fasting...

Choriocarcinoma is a highly malignant tumor that can arise from trophoblasts of any type gestational event but most often complete hydatidiform mole. IGF-II plays fundamental role in placental development and may play trophoblastic diseases. Several studies have shown expressed at high levels moles choriocarcinoma tissues; however, conflicting data exist on how regulates the behaviour cells. The purpose this study was to determine contribution receptors for IGF-I insulin actions regulation...

ABSTRACT We have determined the effect of human insulin-like growth factor-binding protein-1 (hIGFBP-1) on circulating half-life ( t ½ ) factor-I (hIGF-I) and hIGF-I-stimulated 2-deoxyglucose uptake in tissues cannulated conscious rat. The levels hIGF-I rat serum were measured by radioimmunoassay. assay was carried out presence partially purified IGF to remove interference from any IGFBPs not removed acid–ethanol extraction. An intravenous bolus 12·5 μg hIGF-I, given 12-h fasted rats,...

Kaposi's sarcoma (KS) is a highly vascular tumour and the most common neoplasm associated with human immunodeficiency virus (HIV-1) infection. Growth factors, in particular endothelial growth factor (VEGF), have been shown to play an important role its development. The of insulin-like factors (IGFs) pathophysiology different tumours led us evaluate IGF system KS. IGF-I receptors (IGF-IR) were identified by immunohistochemistry biopsies taken from patients AIDS/HIV-related KS stages on KSIMM...

This paper aims to explore student nurses' experiences of bioscience learning, teaching and application within the practice setting. It draws upon social learning theory communities consider issues raised.The many nursing curricula has shifted from traditional more integrated approaches. Student nurses recognise as a valuable component their studies, but find it challenging. The focus previous research in this area often focussed on theoretical rather than settings.A phenomenological...

Amino acid (AA) levels in plasma and erythrocytes (RBC) were determined rats ( n = 29) fed diets with 6, 21, 35% protein, their association insulin-like growth factor I (IGF-I), insulin, or IGF-binding protein (IGFBP)-1 was studied. Free AA RBC by reversed-phase high-pressure liquid chromotography, IGF-I, IGFBP-1, insulin RIA. Rats the low-protein (6%) diet growth-retarded had lower serum IGF-I higher IGFBP-1 than other two groups P &lt; 0.0001). In diet, most of nonessential (NEAA) both...

Previous studies support a role for insulin-like growth factor-binding protein-1 (IGFBP-1) in modulating factor (IGF) availability glucose homeostasis. We have developed radioimmunoassay (RIA) rat IGFBP-1 (rIGFBP-1) and examined the regulation of circulating levels by nutritional hormonal status. Rabbit antisera were raised against pure rIGFBP-1, an assay was established with sensitivity 50 pg. In rat, serum concentrations decrease increasing developmental age. They highest fetal serum,...

Summary Objective IGF‐I has important actions on cell division, apoptosis, differentiation and metabolism, as well proliferation in vascular smooth muscle. Deficiency of GH, an regulator IGF‐I, is associated with reduced well‐being. levels have been related to cognitive function older individuals. The aim the present study was investigate concentrations a normal population men women aged 20–74 years determine influence variety behavioural psychosocial factors metabolic these concentrations....