A5010080458- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant responses to water stress
- Plant Parasitism and Resistance
- Rice Cultivation and Yield Improvement
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Respiratory Support and Mechanisms
- Pluripotent Stem Cells Research
- Advanced Data Storage Technologies
- Cell death mechanisms and regulation
- Blood disorders and treatments
- Hearing Loss and Rehabilitation
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Mechanical Circulatory Support Devices
- Congenital Diaphragmatic Hernia Studies
- Metabolism and Genetic Disorders
- Infectious Encephalopathies and Encephalitis
- Plant Virus Research Studies
- Blood groups and transfusion
- Osteoarthritis Treatment and Mechanisms
- Neurological Complications and Syndromes
- Neuroscience of respiration and sleep
- Collagen: Extraction and Characterization
Nagoya University
1994-2023
Ajinomoto (Japan)
2022
Tohoku University
2014-2018
Fujita Health University
2001-2012
Asparagine is synthesized from glutamine by the reaction of asparagine synthetase (AS) and major nitrogen form in both xylem phloem sap rice (Oryza sativa L.). There are two genes encoding AS, OsAS1 OsAS2, rice, but functions individual AS isoenzymes largely unknown. Cell type- NH4+-inducible expression as well analyses knockout mutants were carried out this study to characterize AS1. was mainly expressed roots, with situ hybridization showing that corresponding mRNA specifically accumulated...
In the final step of cytokinin biosynthesis, main pathway is elimination a ribose-phosphate moiety from nucleotide precursor by phosphoribohydrolase, an enzyme encoded gene named LONELY GUY (LOG). This reaction accounts for most supply needed regulating plant growth and development. contrast, LOG-independent pathway, in which dephosphorylation deribosylation sequentially occur, also thought to play role but entity physiological contribution have been elusive. this study, we profiled...
The development and elongation of active tillers in rice was severely reduced by a lack cytosolic glutamine synthetase1;2 (GS1;2), and, to lesser extent, NADH-glutamate synthase1 knockout mutants. In situ hybridization using the basal part wild-type seedlings clearly showed that expression OsGS1;2 detected phloem companion cells nodal vascular anastomoses large bundles axillary buds. Accumulation lignin, visualized phloroglucin HCl, also observed these tissues. GS1;2 resulted accumulation...
A mutation abolishing cytosolic glutamine synthetase1;2 (GS1;2) activity impairs assimilation of ammonium into in both roots and basal portions shoots, severely decreases axillary bud outgrowth (tillering) mutant rice seedlings. Although the gs1;2 phenotype is independent strigolactone, which inhibits tillering, it also demonstrates glutamine- or related metabolite-responsive biosynthesis cytokinin (CK), promotes tillering. Here, we examined connection between GS1;2 CK during focusing on...
Asparagine is one of the most dominant organic nitrogen compounds in phloem and xylem sap a wide range plant species. Asparaginase (ASNase; EC, 3.5.1.1) catabolizes asparagine into aspartate ammonium; therefore, it suggested to play key role metabolism within legume sink organs. However, metabolic fate source organs during rice seed production remains be elucidated. Therefore, main objective this study investigate temporal spatial manner production. For purpose, expression genes involved...
Our previous transcriptomic analysis revealed that downregulation of nitrogen and carbon metabolism in the basal portions shoots inhibited cytosolic glutamine synthetase1;2 (GS1;2), which severely reduced rice tiller number. In present study, we used mutants lacking GS1;2 (gs1;2 mutants) to determine contribution growth. Metabolomic indicated effects disorder such as reductions levels sugar metabolites (e.g., sucrose glucose 6-phosphate) shoot gs1;2 mutant seedlings. Decrease caused by lack...
Nitrogen is one of the most important elements for plant growth, and urea frequently used nitrogen fertilizers worldwide. Besides exogenously-supplied to soil, endogenously synthesized during secondary metabolism. Here, we investigated contribution a transporter, DUR3, rice production using reverse genetic approach combined with localization studies. Tos17 insertion lines DUR3 showed 50% yield reduction in hydroponic culture, 26.2% paddy field, because decreased grain filling. Because shoot...
Our previous studies concluded that metabolic disorder in the basal portions of rice shoots caused by a lack cytosolic glutamine synthetase1;2 (GS1;2) resulted severe reduction outgrowth tillers. Rice mutants lacking GS1;2 (gs1;2 mutants) showed remarkable contents both and asparagine shoots. In current study, we attempted to reveal mechanisms for this decrease content using either or synthetase 1 (AS1). The contributions availability tillers were investigated. has two AS genes, enzymes...
Abstract Oryza longistaminata, a wild rice, vegetatively reproduces and forms networked clonal colony consisting of ramets connected by rhizomes. Although water, nutrients, other molecules can be transferred between via the rhizomes, inter-ramet communication in response to spatially heterogeneous nitrogen availability is not well understood. We studied ramet pairs using split hydroponic system that allowed each root exposed different conditions. Ammonium uptake was compensatively enhanced...
Oligonucleotide therapeutics have great potential as a next-generation approach to treating intractable diseases. Large quantities of modified DNA/RNA containing xenobiotic nucleic acids (XNAs) must be synthesized before clinical application. In this study, the ancestral RNA ligase AncT4_2 was designed by sequence reconstruction (ASR) perform conjugation reaction fragments. had superior properties native 2 from T4 phage (T4Rnl2), including high productivity, >2.5-fold-higher turnover number,...
Changes of respiration caused by chest wall vibration were studied in 13 patients with cervical cord lesions. Vibrators applied on the intermittently triggered patient's respiratory movements. The vibrators to be three ways. 1) Upper vibrations bilaterally during inspiration 2nd or 3rd parasternal intercostal spaces (insp "in-phase" vibration). 2) Lower expiration 7th-to 10th anterior midaxillary lines (exp 3) Inspiratory and expiratory combined produce alternating phase vibration. Both...
Abstract Oryza longistaminata , a wild rice, vegetatively reproduces and forms networked clonal colony consisting of ramets connected by rhizomes. Although water, nutrients, other molecules can be transferred between via the rhizomes, inter-ramet communication in response to spatially heterogeneous nitrogen availability is not well understood. We studied ramet pairs using split hydroponic system that allowed each root exposed different conditions. Ammonium uptake was compensatively enhanced...