A5071731539- Microstructure and Mechanical Properties of Steels
- DNA Repair Mechanisms
- Metallurgy and Material Forming
- Lung Cancer Diagnosis and Treatment
- Magnetic Properties and Applications
- Workplace Health and Well-being
- Metal Alloys Wear and Properties
- Sleep and Work-Related Fatigue
- Employment and Welfare Studies
- Tracheal and airway disorders
- Occupational Health and Safety Research
- Noise Effects and Management
- Indoor Air Quality and Microbial Exposure
- DNA and Nucleic Acid Chemistry
- Lung Cancer Treatments and Mutations
- Metallurgical Processes and Thermodynamics
- Lung Cancer Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer-related Molecular Pathways
- Ergonomics and Musculoskeletal Disorders
- Medical Imaging and Pathology Studies
- Sleep and related disorders
- Air Quality and Health Impacts
- Health and Wellbeing Research
- Hydrogen embrittlement and corrosion behaviors in metals
Samsung Medical Center
2012-2024
Sungkyunkwan University
2012-2024
Ajou University
2013-2023
Ajou University Hospital
2008-2023
Korean Industrial Economic Association
2023
Hanyang University
2005-2022
Materials Science & Engineering
2001-2020
Park University
2018
The University of Texas Southwestern Medical Center
2006-2016
Radiation Oncology Associates
2009-2013
Abstract Akt phosphorylation has previously been described to be involved in mediating DNA damage repair through the nonhomologous end-joining (NHEJ) pathway. Yet mechanism how stimulates DNA-protein kinase catalytic subunit (DNA-PKcs)-dependent double-strand break (DNA-DSB) not so far. In present study, we investigated by which can interact with DNA-PKcs and promote its function during NHEJ process. The results obtained indicate a prominent role of Akt, especially Akt1 regulation for...
Epidemic outbreaks of multi-drug resistant (MDR) Acinetobacter baumannii (AB) in intensive care units (ICUs) are increasing. The incidence MDR AB bacteremia, which develops as a result colonization, is increasing through widespread dissemination the pathogen, and further colonization. We sought to determine risk factors for bacteremia patients colonized with ICU.We conducted retrospective, observational study 200 ICU at Severance Hospital, South Korea during outbreak period between January...
Multiple DNA double-strand break (DSB) repair pathways are active in S phase of the cell cycle; however, DSBs primarily repaired by homologous recombination (HR) this cycle phase. As non-homologous end-joining (NHEJ) factor, Ku70/80 (Ku), is quickly recruited to phase, we hypothesized that an orchestrated mechanism modulates pathway choice between HR and NHEJ via displacement Ku heterodimer from allow HR. Here, provide evidence phosphorylation at a cluster sites junction pillar bridge...
Phosphorylation of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) upon ionizing radiation (IR) is essential for cellular radioresistance and nonhomologous-end-joining-mediated DNA double-strand break repair. In addition to IR induction, we have previously shown that DNA-PKcs phosphorylation increased camptothecin treatment, which induces replication stress replication-associated breaks. To clarify involvement in this process, analyzed response UV irradiation, causes activates...
The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) plays an important role in DNA double-strand break (DSB) repair as the underlying mechanism non-homologous end joining pathway. When DSBs occur, DNA-PKcs is rapidly phosphorylated at both Thr-2609 and Ser-2056 residues, such phosphorylations are critical for DSB repair. In this study we report that, addition to responding DSBs, activated normal cell cycle progression through mitosis. Mitotic induction phosphorylation closely...
The Cryptochrome (CRY) proteins are critical components of the mammalian circadian clock and act to rhythmically repress activity transcriptional activators CLOCK BMAL1 at heart mechanism. CRY part a large repressive complex, which not completely known. Using mass spectroscopy, we identified catalytic subunit DNA-dependent protein kinase as CRY-interacting found that loss or inhibition this results in rhythms with abnormally long periods. We then serine 588 C-terminal tail mouse CRY1...
Background: Presenteeism, a concept that has recently undergone active study, is the act of attending work while sick.This study investigates association between presenteeism and various psychosocial factors within workplaces.Methods: This analyzed 29246 wage earners from third Korean Working Conditions Survey (KWCS, 2011) data using logistic regression analysis to investigate workplaces.Results: Among earners, 6347 (21.7 %) showed presenteeism.Those who experienced age discrimination at...
Abstract Background Interferon-γ release assay (IGRA) may improve diagnostic accuracy for latent tuberculosis infection (LTBI). This study compared the performance of tuberculin skin test (TST) with that IGRA diagnosis LTBI in immunocompromised patients an intermediate TB burden country where BCG vaccination is mandatory. Methods We conducted a retrospective observational given TST and IGRA, QuantiFERON-TB Gold In-Tube (QFT-IT), at Severance Hospital, tertiary hospital South Korea, from...
DNA double strand breaks (DSB) are repaired by nonhomologous end-joining (NHEJ) or homologous recombination (HR). Recent genetic data in yeast shows that the choice between these two pathways for repair of DSBs is via competition NHEJ protein, Ku, and HR Mre11/Rad50/Xrs2 (MRX) complex. To study interrelationship human Ku Mre11 Mre11/Rad50 (MR), we established an vitro end resection system using a forked model dsDNA substrate purified Ku70/80, Mre11, Mre11/Rad50, exonuclease 1 (Exo1). Our...
To investigate the association between serum perfluorooctanoic acid (PFOA) concentration and cardiovascular disease, as measured by homocysteine level blood pressure in a representative sample of US adults.A cross-sectional study 2934 adults (≥20 years) who participated 2003-2004 2005-2006 National Health Nutrition Examination Survey had detectable levels PFOA their serum. The health effects analysed potentially associated with exposure included pressure.The geometric mean value (95% CI)...
XLF physically interacts with XRCC4 and Ku by anti tag coimmunoprecipitation (View interaction).
Patient- and procedure-related factors associated with postoperative pulmonary complications (PPCs) have changed over the last decade. Therefore, we sought to identify independent risk of PPCs develop a clinically applicable scoring system. We retrospectively analyzed clinical data from 2,059 patients who received preoperative evaluations respiratory physicians between June 2011 October 2012. A new system for estimating was developed using beta coefficients final multiple regression models....
The heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) has been implicated in telomere protection and telomerase activation.Recent evidence further demonstrated that hnRNP-A1 plays a crucial role maintaining newly replicated telomeric 3 overhangs facilitating the switch from replication protein A (RPA) to of telomeres 1 (POT1).The also involves DNA-dependent kinase catalytic subunit (DNA-PKcs), although detailed regulation mechanism not clear.Here we report is phosphorylated by DNA-PKcs...
Repair of DNA double-strand breaks in mammalian cells occurs via a direct nonhomologous end-joining pathway. Although this pathway can be studied vivo and crude cell-free systems, deeper understanding the mechanism requires reconstitution with purified enzymes. We have expressed complex two proteins that are critical for break repair, ligase IV (DNL IV) XRCC4. The is homogeneous, molecular mass about 300,000 Da, suggestive mixed tetramer containing copies each polypeptide. presence multiple...
We evaluated the utility of a combined approach using endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and transesophageal bronchoscopic fine-needle (EUS-FNA-B/E) for mediastinal staging lung cancer.An EBUS-TBNA database was analyzed retrospectively. EUS-FNA-B/E performed after when lymph nodes were not accessible or tissue sampling alone inadequate.During study period, 44 patients enrolled. on 79 52 nodes, respectively. The sensitivity, specificity, accuracy...
Non-homologous end-joining (NHEJ) and homologous recombination (HR) are the two prominent pathways responsible for repair of DNA double-strand breaks (DSBs). NHEJ is not restricted to a cell-cycle stage, whereas HR active primarily in S/G2 phases suggesting there cell cycle-specific mechanisms that play role choice between HR. Here we show attenuated S phase via modulation autophosphorylation status factor DNA-PKcs at serine 2056 by pro-HR BRCA1. BRCA1 interacts with cycle-regulated manner...
XLF/Cernunnos is a core protein of the nonhomologous end-joining pathway DNA double-strand break repair. To better define role Cernunnos in end joining, whole-cell extracts were prepared from Cernunnos-deficient human cells. These effected little joining ends with cohesive 5′ or 3′ overhangs, and no at all partially complementary overhangs that required gap filling prior to ligation. Assays which gap-filled but unligated intermediates trapped using dideoxynucleotides revealed there was on...