Abstract Hesperidin, a flavonoid derived from citrus fruits, has been reported to show various biological effects including anticancer activity. This study investigated whether hesperidin affected the proliferation of MCF‐7 human breast cancer cells transfected with green fluorescent protein (GFP)/ α ‐tubulin (MCF‐7‐GFP‐Tubulin cells), androgen‐independent PC‐3 and DU‐145 prostate cells, androgen‐dependent LNCaP cells. The results were as follows. (1) Hesperidin inhibited MCF‐7‐GFP‐Tubulin...

Niclosamide, an FDA-approved anthelmintic drug, has been characterized as a potent Wnt inhibitor that can suppress tumor growth and cancer stem-like cell (CSC) populations. However, the underlying molecular mechanisms remain poorly understood. This study aimed to examine how inhibition by niclosamide preferentially targets CSCs.The mechanistic role of in CSC was examined public databases, human colorectal cells, xenografts, azoxymethane/dextran sulfate sodium (AOM/DSS)-induced...

In this study, we investigated the effects of flavonoid galangin on expression mucin 5AC (MUC5AC) gene in airway cells. Human pulmonary epithelial NCI-H292 cells were pretreated with for 30 min and then stimulated phorbol 12-myristate 13-acetate (PMA) 24 h. We also examined PMA-induced nuclear factor-κB (NF-κB) signaling pathway. Galangin inhibited production glycoproteins MUC5AC mRNA induced by PMA via prevention NF-κB inhibitor α degradation p65 translocation. These findings indicated that...

A simplified cochlear implant (CI) system would be appropriate for widespread use in developing countries. Here, we describe a CI that have designed to realize such concept. The implements 8 channels of processing and stimulation using the continuous interleaved sampling (CIS) strategy. generic digital signal (DSP) chip is used processing, filtering functions are performed with fast Fourier transform (FFT) microphone or other input. Data derived from transmitted through an inductive link...

This study investigated whether genistein and curcumin affect epidermal growth factor (EGF)-induced MUC5AC mucin production gene expression from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with each agent for 30 min then stimulated EGF 24 h. The protein measured by reverse transcription - polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent assay (ELISA), respectively. results as follows: (1) inhibited the of induced EGF, dose-dependently; (2) also EGF....

We examined whether apigenin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well vivo production MMP-3 knee joint rat to evaluate potential chondroprotective effects apigenin. Rabbit chondrocytes were a monolayer, reverse transcription - polymerase chain reaction (RT-PCR) was used measure interleukin-1β (IL-1β)-induced expression MMP-3, MMP-1, MMP-13, disintegrin metalloproteinase with...

The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls presence of pungency, in 121 F2 individuals from a cross between 'ECW123R' and 'CM334', both Capsicum annuum. We concluded that CS are tightly linked. Sequence analysis genes four pungent non-pungent pepper lines showed peppers had 2,529 bp-deletion 5′upstream region CS. have developed molecular markers locus to detect pungency at seedling stage. Based on deleted sequence, we five SCAR markers, two...

It is a traditional teaching that the levator aponeurosis main transmitter of palpebrae muscle. However, there are several points raise doubts in this fundamental concept as being primary interconnecting mechanism upper lid elevation. Despite structural integrity complex, drooping eyelids seen to develop situations such Horner's syndrome and times excessive fatigue sleepiness. Amid controversy literature regarding specific role lid-elevating mechanism, we have observed fails make constant...

Bilberry (European blueberry) has been reported to have many biological effects, including anticancer activity. In this study, we investigated the antiproliferative effects of bilberry extract in relation its ability induce apoptosis and affect microtubule assembly organization MCF7 human breast cancer cells. We observed that inhibited cell proliferation a concentration-dependent fashion with 50% inhibitory concentration 0.3-0.4 mg/mL, concert induction apoptotic death. At these...

We investigated whether luteolin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well production MMP-3 rat knee to evaluate potential chondro-protective effects luteolin. Rabbit chondrocytes were a monolayer IL-1β-induced expression levels MMP-3, MMP-1, MMP-13, disintegrin metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 type II collagen measured by reverse transcription -...

In the present study, we investigated whether apigenin significantly affects tumor necrosis factor-α (TNF-α)-induced production and gene expression of MUC5AC mucin in airway epithelial cells. Confluent NCI-H292 cells were pretreated with for 30 min then stimulated TNF-α 24 h or indicated periods. The protein measured by reverse transcription - polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent assay (ELISA), respectively. Apigenin inhibited down-regulated induced To elucidate...

We evaluated the chondroprotective effects of wogonin by investigating its on gene expression and production matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well MMP-3 rat knee. Rabbit chondrocytes were a monolayer, RT-PCR was used to measure interleukin-1β (IL-1β)-induced MMP-3, MMP-1, MMP-13, disintegrin metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), type II collagen. In IL-1β-induced proteolytic activity investigated using western blot...

Abstract Background Lipid rafts (LRs), cholesterol‐enriched microdomains on cell membranes, are increasingly viewed as signalling platforms governing critical facets of cancer progression. The phenotype stem‐like cells (CSCs) presents significant hurdles for successful treatment, and the expression several CSC markers is associated with LR integrity. However, implications in CSCs remain unclear. Methods This study evaluated biological molecular functions LRs colorectal (CRC) by using an...

Baicalein, berberine, curcumin and hesperidin are the major components derived from Scutellaria baicalensis, Coptis japonica, Curcuma longa Poncirus trifoliata, respectively. These plants have been used for treatment of diverse chronic inflammatory diseases including respiratory disease in oriental medicine their respective were reported to various biological effects anti-inflammatory activity. In present study, we investigated whether these four natural products affect mucin release airway...

Background: This study was proposed to evaluate the facial contour and electrophysiologic changes of masseter temporalis muscles before after botulinum toxin A injection in wide lower face (square face). Methods: The injections were performed on 10 patients for treatment square with hypertrophy. To obtain an objective evaluation change contour, physical measurements, cephalometry, clinical photographs taken; function muscles, electromyographic studies 1, 3, 5, 7, 9, 12 months treatment....

In this study, we investigated whether wogonin significantly affects MUC5AC mucin gene expression and production in human airway epithelial cells. Confluent NCI-H292 cells were pretreated with for 30 min then stimulated tumor necrosis factor-α (TNF-α) 24 h or the indicated periods. The protein measured by RT-PCR ELISA, respectively. We found that incubation of inhibited down-regulated induced TNF-α a dose-dependent fashion. To elucidate action mechanism wogonin, effect on TNF-α-induced NF-κB...

Background It is valuable to find the potential activity of regulating excessive mucin secretion by compounds derived from various medicinal plants. We investigated whether aqueous extract root bark Morus alba L. (AMA), kuwanon E, G, mulberrofuran and morusin significantly affect production airway using in vivo vitro experimental models. Methods Effect AMA was examined on hypersecretion sulfur dioxide-induced acute bronchitis rats. Confluent NCI-H292 cells were pretreated with ethanolic...