In this work, it is proposed an environmental friendly sonophotocatalytic approach to efficiently treat polluted waters from industrial dyes exploiting ZnO micro- and nano-materials. For the first time, we deeply investigated generation of reactive oxygen species (ROS) under ultrasound stimulation different structures by Electron Paramagnetic Resonance Spectroscopy (EPR). Indeed, five zinc oxide (ZnO) nano-structures, i.e. Desert Roses (DRs), Multipods (MPs), Microwires (MWs), Nanoparticles...

At present, ultrasound radiation is broadly employed in medicine for both diagnostic and therapeutic purposes at various frequencies intensities. In this review article, we focus on therapeutically-active nanoparticles (NPs) when stimulated by ultrasound. We first introduce the different ultrasound-based therapies with special attention to techniques involved oncological field, then summarize NPs used, ranging from soft materials, like liposomes or micro/nano-bubbles, metal oxide NPs....

In the present paper, we use zinc oxide nanoparticles under excitation of ultraviolet (UV) light for generation Reactive Oxygen Species (ROS), with aim further using these species fighting cancer cells in vitro. Owing to difficulties obtaining highly dispersed (NPs) biological media, propose their coating a double-lipidic layer and evaluate colloidal stability comparison pristine NPs. Then, Electron Paramagnetic Resonance (EPR) coupled spin-trapping technique, demonstrate characterize...

The surface chemistry and charge of zinc oxide nanocrystals influence their behaviour in biological fluids. A novel lipid bilayer assembly is developed to shield ZnO improving stability cell internalization.

Herein we report a novel, easy, fast and reliable microwave-assisted synthesis procedure for the preparation of colloidal zinc oxide nanocrystals (ZnO NCs) optimized biological applications. ZnO NCs are also prepared by conventional solvo-thermal approach properties two families compared discussed. All fully characterized in terms morphological analysis, crystalline structure, chemical composition optical properties, both as pristine nanomaterials or after amino-propyl group...

Aim:The effective application of nanoparticles in cancer theranostics is jeopardized by their aggregation biological media, rapid degradation and clearance.The design biomimetic nanoconstructs with enhanced colloidal stability non-immunogenicity therefore essential.We propose naturally stable cell-derived extracellular vesicles to encapsulate zinc oxide (ZnO) nanocrystals as efficacious nanodrugs, obtain highly Trojan nano-horses (TNHs).Materials & methods: Coupling efficiency, biostability,...

In the last years, different nanotools have been developed to fight cancer cells. They could be administered alone, exploiting their intrinsic toxicity, or remotely activated achieve cell death. latter case, ultrasound (US) has recently proposed stimulate some nanomaterials because of US outstanding property deep tissue penetration and possibility focusing. this study, for first time, we report on highly efficient killing capability amino-propyl functionalized ZnO nanocrystals (ZnO NCs) in...

We propose an efficient method to modify B-cell derived EVs by loading them with a nanotherapeutic stimuli-responsive cargo and equipping antibodies for targeting of lymphoma cells.The post-isolation engineering the is accomplished freeze-thaw load therapeutically-active zinc oxide nanocrystals (ZnO NCs), obtaining so-called TrojanNanoHorse (TNH) recall biomimetism cytotoxic potential this novel nanoconstruct. TNHs are further modified at their surface anti-CD20 monoclonal (TNHCD20)...

Nanomaterials for bone tissue engineering are developing to new solutions against infections, antibiotic resistance, promotion of bone. ZnO nanocrystals promising candidates with intrinsic antimicrobial activity and high biocompatibility.

Cellular communications take place thanks to a well-connected network of chemical–physical signals, biomolecules, growth factors, and vesicular messengers that travel inside or between cells. A deep knowledge the extracellular vesicle (EV) system allows for better understanding whole series phenomena responsible cell proliferation death. To this purpose, here, thorough immuno-phenotypic characterization B-cell EV membranes is presented. Furthermore, cellular membrane B lymphocytes, Burkitt...

In recent years, there has been a breakthrough in the integration of artificial nanoplatforms with natural biomaterials for development more efficient drug delivery systems. The formulation bioinspired nanosystems, combining benefits synthetic nanoparticles features biological materials, provides an strategy to improve nanoparticle circulation time, biocompatibility and specificity toward targeted tissues. Among others extracellular vesicles (EVs), membranous structures secreted by many...

Nanoparticles able to promote inertial cavitation when exposed focused ultrasound have recently gained much attention due their vast range of possible applications in the biomedical field, such as enhancing drug penetration tumor or supporting contrast imaging. Due nanometric size, these agents could penetrate through endothelial cells vasculature target tissues, thus enabling higher imaging resolutions than commercial gas-filled microbubbles. Herein, Zinc Oxide NanoCrystals (ZnO NCs),...

Nanomedicine is an emerging treatment approach for many cancers, characterized by having high sensitivity and selectivity tumor cells minimal toxic effects induced the conventional chemotherapeutics. In these context, smart nanoparticles (NPs) are getting increasingly relevant in development of new therapies. NPs with specific chemical composition and/or structure being stimuli-responsive to magnetic, light or ultrasound waves promising tools. present work, amorphous-titania propyl-amine...

Abstract Despite different nanomaterials were developed so far against cancer, their potential drawbacks are still scarcely considered. The off-target delivery of a therapeutic compound, as well the non-specific uptake these by healthy tissues or organs, and immunogenicity some major issues that have to be faced prior successful clinical translation. This work aims develop an innovative theranostic, biocompatible, drug-loaded nanoconstruct based on Gadolinium-doped Zinc Oxide (ZnO-Gd)...

Abstract Ultrasound (US), is gaining considerable interest as therapy and diagnostic tool, being safe, deep‐tissue penetrating, enabling variegate interventions. Although some US applications have already reached the clinical practice, innovative interventions combining them to microbubbles, nanoparticles, scaffolds novel imaging techniques face complex translation. Here technologies are illustrated in 3D cell structures: in‐vitro systems at different levels of complexity, models can fairly...

Colorectal Cancer (CRC) is the second leading cause of death among tumors worldwide. Conventional treatments are often accompanied by emerging immunotherapies, trying to reduce burden advanced and metastatic stages. Recently, nanomedicine therapies have been under intensive research offer new perspectives patients. Motivated this rationale, work proposes formulation biomimetic targeted nanoparticles (NPs) enabling a stimuli responsive localized therapy, triggered safe use acoustic...

In the last few years, nanotechnology has revolutionized potential treatment of different diseases. However, use nanoparticles for drug delivery might be limited by their immune clearance, poor biocompatibility and systemic immunotoxicity. Hypotheses overcoming rejection from body increasing include coating with cell membranes. Additionally, source cell-specific targeting been reported when tumor cells Here we show that mesoporous silica membranes derived preosteoblastic could employed to...