Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in brain. With advances multi-omics datasets, a major challenge is to validate and integrate results into biological understanding spatial organization functional orientation. Here, we generate transcriptomes metabolites from six patients with brain trauma surgical samples. The resulting marker gene, which highly replicable across analysis methods, sequencing...

Stroke is a leading cause of long-lasting disability in humans. However, currently there are still no effective therapies available for promoting stroke recovery. Recent studies have shown that the adult brain has capacity to regenerate neurons after stroke. Although this neurogenic response may be functionally important repair injury, mechanisms underlying stroke-induced neurogenesis not known. Caspase-3 major executioner and been identified as key mediator neuronal death acute stage...

The study aims to investigate the effects of long noncoding RNA (lncRNA) transmitted nuclear factor-κB interacting lncRNA (NKILA)-containing astrocyte-derived small extracellular vesicles (EVs) on traumatic brain injury (TBI). TBI was modeled in vitro by exposing human neurons mechanical and vivo controlled cortical impact a mouse model. gain- loss-function approaches were conducted injured explore role NKILA, microRNA-195 (miR-195) nucleotide-binding leucine-rich repeat containing family...

Background The poor prognosis of sepsis warrants the investigation biomarkers for predicting outcome. Several studies have indicated that PANoptosis exerts a critical role in tumor initiation and development. Nevertheless, has not been fully elucidated. Methods We obtained Sepsis samples scRNA-seq data from GEO database. PANoptosis-related genes were subjected to consensus clustering functional enrichment analysis, followed by identification differentially expressed calculation score. A...

Rice (Oryza sativa) is the leading source of nutrition for more than half world's population, and by far it most important commercial food crop. one staple crops around world. But, its growth production are significantly hampered bacterial pathogen Xanthomonas oryzae pv. (Xoo) which causes leaf blight diseaserice disease caused (Xoo). Earlier studies have reported antibacterial ability FDA-approved niclosamide drug against Xoo. However, underlying mechanism blocks Xoo remained elusive. In...

Abstract Aims Despite the success of single‐cell RNA sequencing in identifying cellular heterogeneity ischemic stroke, clarifying mechanisms underlying these associations differently expressed genes remains challenging. Several studies that integrate gene expression and quantitative trait loci (eQTLs) with genome wide‐association study (GWAS) data to determine their causal role have been proposed. Methods Here, we combined Mendelian randomization (MR) framework single cell (sc) how (DEGs)...

Abstract Demyelination is a common feature of numerous neurological disorders including multiple sclerosis and leukodystrophies. Although myelin can be regenerated spontaneously following injury, this process often inadequate, potentially resulting in neurodegeneration exacerbating dysfunction. Several drugs aimed at promoting the differentiation oligodendrocyte precursor cells (OPCs) have yielded unsatisfactory clinical effects. A recent study has shifted strategy pro-OPC towards enhancing...

Toripalimab, a humanized anti-PD-1 monoclonal antibody, is widely employed in the treatment of non-small cell lung cancer (NSCLC) and various other malignancies. However, there have been no reported cases linking prolonged administration toripalimab to immune-mediated hepatitis (IMH). Typically, immune checkpoint inhibitor (ICI)-related IMH manifests within first few weeks or months following initiation therapy. In this report, we presented case patient with NSCLC ~ 17 treatment. induced by...

Microglia‑mediated neuroinflammation is key in the pathogenesis of Alzheimer's disease (AD). Several studies have suggested that NADPH oxidase contributes to microglia‑mediated neuroinflammation. Resveratrol, which a natural polyphenolic compound, exerts neuroprotective effects AD due its anti‑inflammatory properties. The present study aimed investigate resveratrol on activation oligomeric amyloid β (oAβ)‑induced BV‑2 microglia, and determine role these effects. Microglial proliferation was...

Air pollution has been a serious health issue in Beijing for years. Airborne antibiotic-resistant bacteria could be potential crisis as reserve of antibiotic resistance transmission environment. The composition and pattern culturable bacterial community how these are affected by air remain unclear. This study aimed to compare the compositions patterns polluted non-polluted weather conditions Beijing. samples were collected indoors outdoors during using six-stage Andersen Samplers. For each...

Microglial activation and neuroinflammation induced by amyloid β (Aβ) play pivotal roles in Alzheimer's disease (AD) pathogenesis. Astragaloside IV (AS-IV) is one of the major active compounds traditional Chinese medicine Astmgali Radix. It has been reported that AS-IV could protect against Aβ-induced cognitive impairment, but underlying mechanisms need to be further clarified. In this study, therapeutic effects were investigated an oligomeric Aβ (oAβ) AD mice model. The on microglial...

Abstract Histologic grade is one of the most important microscopic features used to predict prognosis invasive breast cancer and may serve as a marker for studying driving genomic abnormalities in vivo . We analyzed whole genome sequencing data from 680 cases TCGA ductal carcinomas correlated them corresponding pathology information. Ten genetic were found be statistically associated with histologic grade, including three prevalent driver events, TP53 PIK3CA mutations MYC amplification. A...

Exogenous insulin like growth factor-1 (IGF-1) is known to be neuroprotective in animal models with brain insults, while it can also cause hyperexcitability rodents. In this regard, the role of endogenous IGF-1 responses insults excitotoxicity, a common pathology injuries, remains elucidated. Here, we investigated potential cell-specific kainic acid (KA) -induced degeneration neurons.Kainic was given primary cultured cortical neurons and co-cultured astrocytes were added as supportive...

Introduction Post-traumatic coagulopathy (PTC) is a critical pathology in traumatic brain injury (TBI), however, its potential mechanism not clear. To explore this peripheral samples, we integrated single cell RNA-sequencing and T repertoire (TCR)-sequencing across cohort of patients with TBI. Methods Clinical samples from more severity demonstrated overexpression receptor–encoding genes less TCR diversity. Results By mapping clonality, found PTC have clones, the clones are mainly...

Circular RNAs (circRNAs) are abundant in the brain and contribute to central nervous system diseases; however, exact roles of circRNAs human traumatic injury (TBI) have not been established. In this study, we used a competing endogenous RNA (ceRNA) chipset as well vitro vivo assays characterize differentially expressed TBI. We detected 3035 severe TBI group, 2362 moderate 433 mild group. A ceRNA network was constructed. The circRNA has_circ_0020269 (circHtra1) significantly upregulated after...

Long non-coding RNAs (lncRNAs) and messenger (mRNAs) play an important role in central nervous diseases; however, the exact expression co-expressed profiles human traumatic brain injury (TBI) are still unknown. Therefore, we investigated whole blood 12 patients with TBI 4 healthy controls to observe characteristics different severity. We identified 3,035 lncRNAs 1,204 mRNAs differentially expressed severe group, 2,362 656 moderate 433 100 mild group. Enrichment analyses showed 30 signaling...

Defective brain hormonal signaling and autophagy have been associated with neurodegeneration after insults, characterized by neuronal loss cognitive dysfunction. However, few studies linked them in the context of injury. Insulin-like growth factor-1 (IGF-1) is an important hormone that contributes to growth, cell proliferation, also expressed brain. Here, we assessed clinical data from TBI patients performed both vitro vivo experiments proteomic gene-chip analysis assess functions IGF-1...

Although more and evidence has supported that metabolic syndrome (MS) is linked to ischemic stroke (IS), the molecular mechanism genetic association between them not been investigated. Here, we combined existing single-cell RNA sequencing (scRNA-seq) data mendelian randomization (MR) for understand role of dysregulated metabolism in stroke. The shared hub genes were identified with machine learning WGCNA. A total six upregulated DEGs five downregulated selected subsequent analyses. Nine...