A5088870813- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- TGF-β signaling in diseases
- Spaceflight effects on biology
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Polysaccharides and Plant Cell Walls
- Phagocytosis and Immune Regulation
- Protein Kinase Regulation and GTPase Signaling
- GDF15 and Related Biomarkers
- Metalloenzymes and iron-sulfur proteins
- Genetic factors in colorectal cancer
- Fungal Biology and Applications
- Microtubule and mitosis dynamics
- Enzyme Production and Characterization
- Nanoparticles: synthesis and applications
- Nuclear Receptors and Signaling
- Connective Tissue Growth Factor Research
- Evolution and Genetic Dynamics
- Congenital heart defects research
- Digestive system and related health
Texas Woman's University
2015-2023
Texas A&M Health Science Center
2010-2015
Texas A&M University
2014-2015
College Station Medical Center
2013
Texas A&M University System
2012
Rutgers, The State University of New Jersey
2002-2010
Cold Spring Harbor Laboratory
1999-2001
State University of New York
1999-2001
Stony Brook University
2000-2001
Development of the nematode Caenorhabditis elegans is highly reproducible and fate every somatic cell has been reported. We describe here a previously uncharacterized in C. elegans: we show that germ cells, which hermaphrodites can differentiate into sperm oocytes, also undergo apoptotic death. In adult hermaphrodites, over 300 cells die, using same execution machinery (ced-3, ced-4 ced-9) as described 131 deaths. However, this activated by distinct pathway, loss egl-1 function, inhibits...
Abstract Development of the nematode Caenorhabditis elegans is highly reproducible and fate every somatic cell has been reported. We describe here a previously uncharacterized in C. elegans: we show that germ cells, which hermaphrodites can differentiate into sperm oocytes, also undergo apoptotic death. In adult hermaphrodites, over 300 cells die, using same execution machinery (ced-3, ced-4 ced-9) as described 131 deaths. However, this activated by distinct pathway, loss egl-1 function,...
Abstract Background Bone morphogenetic proteins (BMPs) are members of the conserved transforming growth factor β (TGFβ superfamily, and play many developmental homeostatic roles. In C. elegans , a BMP-like pathway, DBL-1 controls body size is involved in innate immunity. How these functions carried out, though, what most downstream targets this pathway are, remain unknown. Results We performed microarray analysis compared expression profiles animals lacking SMA-6 receptor, which decreases...
In mammals, Bone Morphogenetic Protein (BMP) pathway signaling is important for the growth and homeostasis of extracellular matrix, including basement membrane remodeling, scarring, bone growth. A conserved BMP member in Caenorhabditis elegans, DBL-1, regulates body length a dose-sensitive manner. Loss DBL-1 also results increased anesthetic sensitivity. However, physiological basis these pleiotropic phenotypes largely unknown. We created over-expressing strain show that sensitivity to...
Bone morphogenetic protein (BMP) pathways control an array of developmental and homeostatic events, must themselves be exquisitely controlled. Here, we identify Caenorhabditis elegans SMA-10 as a positive extracellular regulator BMP–like receptor signaling. acts genetically in (Sma/Mab) pathway between the ligand DBL-1 its receptors SMA-6 DAF-4. We cloned sma-10 show that it has fifteen leucine-rich repeats three immunoglobulin-like domains, hallmarks LRIG subfamily transmembrane proteins....
The cuticle of C. elegans is a highly resistant structure that surrounds the exterior animal1-4. not only protects animal from environment, but also determines body shape and plays role in motility4-6. Several layers secreted by epidermal cells comprise cuticle, including an outermost lipid layer7. Circumferential ridges called annuli pattern length are present during all stages development8. Alae longitudinal specific development, L1, dauer, adult stages2,9. Mutations genes affect cuticular...
Programmed cell death, which occurs through a conserved core molecular pathway, is important for fundamental developmental and homeostatic processes. The human iron–sulfur binding protein NAF-1/CISD2 binds to Bcl-2 its disruption in cells leads an increase apoptosis. Other members of the CDGSH iron sulfur domain (CISD) family include mitoNEET/CISD1 Miner2/CISD3. In humans, mutations CISD2 result Wolfram syndrome 2, disease patients display juvenile diabetes, neuropsychiatric disorders...
Abstract Mitogen‐activated protein kinases (MAPK) are critical mediators of cellular responses to pathogens and activated in response infection, but investigation is difficult multi‐cell hosts due developmental lethality mutations. Mycobacterium marinum ( Mm ) an established model for tuberculosis, a disease afflicting nearly one‐third the world's population. We found that ‐infected Caenorhabditis elegans display >80% mortality, nonpathogenic M. smegmatis cause <15% mortality. C....
Cellular responsiveness to environment, including changes in extracellular matrix (ECM), is critical for normal processes such as development and wound healing, but can go awry, oncogenesis fibrosis. One type of molecular pathway contributing this the BMP signaling pathway. Owing their broad potent functions, BMPs pathways are regulated at multiple levels. In Caenorhabditis elegans, ligand DBL-1 a regulator body size. We previously showed that DBL-1/BMP determines size through...
How BMP signaling and other body size regulators interact is not clear. We found interactions between Caenorhabditis elegans DBL-1/BMP ECM, proteins that may modify or secrete DBL-1, the SET domain protein BLMP-1. DBL-1 control downstream targets, some through BLMP-1, affect either directly by feeding back on signaling.
Regulated intercellular signaling is critical for the normal development and maintenance of multicellular organisms. Glypicans have been shown to regulate by TGFβs, hedgehogs Wnts, in several cellular contexts. comprise a conserved family heparan sulfated, glycosylphosphatidylinositol (GPI)-linked extracellular proteins. The structural complexity glypicans may underlie their functional complexity. In recent study(31), we built on previous findings that one two C. elegans glypicans, LON-2,...
Generating specific, robust protective responses to different bacteria is vital for animal survival. Here, we address the role of transforming growth factor β (TGF-β) member DBL-1 in regulating signature host defense Caenorhabditis elegans human opportunistic Gram-negative and Gram-positive pathogens. Canonical signaling required suppress avoidance behavior response Gram-negative, but not bacteria. We propose that absence DBL-1, animals perceive some as more harmful. Animals activate pathway...
C. elegans has proven to be a valuable model system for the discovery and functional characterization of many genes gene pathways. More sophisticated tools resources studies in this are facilitating continued with more subtle phenotypes or roles. Here we present generalized protocol adapted identifying postembryonic interest using RNAi. This procedure is easily modified assay phenotype choice, whether by light fluorescence optics on dissecting compound microscope. screening capitalizes...
The cuticle of C. elegans is a highly resistant structure that surrounds the exterior animal1-4. not only protects animal from environment, but also determines body shape and plays role in motility4-6. Several layers secreted by epidermal cells comprise cuticle, including an outermost lipid layer7. Circumferential ridges called annuli pattern length are present during all stages development8. Alae longitudinal specific development, L1, dauer, adult stages2,9. Mutations genes affect cuticular...
Cell size is an important determinant of body size. While the genetic mechanisms cell regulation have been well studied in yeast, this process has only recently addressed multicellular organisms. One recent report by Wang et al. (2002) shows that nematode C. elegans, TGFbeta-like pathway acts hypodermis to regulate and consequently size.1 This finding exciting step discovering molecular control