A5037226224- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
- Planarian Biology and Electrostimulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Gut microbiota and health
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Coral and Marine Ecosystems Studies
- Urinary Tract Infections Management
- Urinary Bladder and Prostate Research
- Pancreatic function and diabetes
- Marine Invertebrate Physiology and Ecology
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Marine Sponges and Natural Products
- Zebrafish Biomedical Research Applications
- Marine and coastal plant biology
Centre for Genomic Regulation
2021-2024
Universitat Pompeu Fabra
2021-2023
University of Zagreb
2020
Stony corals are colonial cnidarians that sustain the most biodiverse marine ecosystems on Earth: coral reefs. Despite their ecological importance, little is known about cell types and molecular pathways underpin biology of reef-building corals. Using single-cell RNA sequencing, we define over 40 across life cycle Stylophora pistillata. We discover specialized immune cells, uncover developmental gene expression dynamics calcium-carbonate skeleton formation. By simultaneously measuring...
Cyclic GMP-AMP synthase (cGAS) is an enzyme in human cells that controls immune response to cytosolic DNA. Upon binding DNA, cGAS synthesizes a nucleotide signal 2′3′-cGAMP activates STING-dependent downstream immunity. Here, we discover cGAS-like receptors (cGLRs) constitute major family of pattern recognition innate Building on recent analysis Drosophila, identify >3,000 cGLRs present nearly all metazoan phyla. A forward biochemical screening 150 animal reveals conserved mechanism...
The assembly of the neuronal and other major cell type programs occurred early in animal evolution. We can reconstruct this process by studying non-bilaterians like placozoans. These small disc-shaped animals not only have nine morphologically described types no neurons but also show coordinated behaviors triggered peptide-secreting cells. investigated possible affinities these peptidergic cells using phylogenetics, chromatin profiling, comparative single-cell genomics four found conserved...
Abstract Single-cell sequencing technologies are revolutionizing biology, but they limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and sortable permeable. As proof of principle, provide transcriptomic data different species, using both droplet-based combinatorial barcoding...
Sequence variants in cis-acting enhancers are important for polygenic disease, but their role Mendelian disease is poorly understood. Redundancy between that regulate the same gene thought to mitigate pathogenic impact of enhancer mutations. Recent findings, however, have shown loss-of-function mutations a single near PTF1A cause pancreas agenesis and neonatal diabetes. Using mouse human genetic models, we show this activates an entire cluster early pancreatic multipotent progenitors. This...
Abstract The emergence of new structures can often be linked to the evolution novel cell types that follows rewiring developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared other chordate clades and question remains whether how vertebrate morphological innovations related appearance embryonic populations. We previously proposed, studying mesoderm development in cephalochordate amphioxus, scenario for head mesoderm. To further test this at...
We studied the effect of microbiota on transcriptome and weight urinary bladder by comparing germ-free (GF) specific pathogen-free (SPF) housed mice. In total, 97 genes were differently expressed (fold change > ±2; false discovery rate (FDR) p-value < 0.01) between groups, including regulating circadian rhythm (Per1, Per2 Per3), extracellular matrix (Spo1, Spon2), neuromuscular synaptic transmission (Slc18a3, Slc5a7, Chrnb4, Chrna3, Snap25). The highest increase in expression was observed...
Abstract The emergence of new structures can often be linked to the evolution novel cell types that follows rewiring developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared other chordate clades and question remains whether how vertebrate morphological innovations related appearance embryonic populations. We already proposed, studying mesoderm development in cephalochordate amphioxus, scenario for head mesoderm. To further test this at...