A5102935011- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Liver physiology and pathology
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Mechanisms and Therapy
- Amino Acid Enzymes and Metabolism
- Cancer Immunotherapy and Biomarkers
- Helicobacter pylori-related gastroenterology studies
- Ferroptosis and cancer prognosis
- Organ Transplantation Techniques and Outcomes
- Immune Response and Inflammation
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Inflammatory mediators and NSAID effects
- Cancer-related gene regulation
- NF-κB Signaling Pathways
- Synthesis and biological activity
- Gastrointestinal Tumor Research and Treatment
- Cell death mechanisms and regulation
- Lymphatic System and Diseases
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Gallbladder and Bile Duct Disorders
Fujian Medical University
2013-2025
Union Hospital
2013-2025
Abstract In recent years, immune checkpoint inhibitor has achieved remarkable success in multiple cancer treatment. However, how to pre‐judge which patients are suitable for is a difficult problem. We use the existing public bioinformatics database comprehensively analyze relationship between clinical data of various cancers with blocking molecules and long non‐coding RNAs (lncRNAs), try find potential predictive value lncRNA immunotherapy inhibitors. this study, we found that: (a) high...
Abstract: Prostaglandin E2 (PGE2) plays a crucial role in inflammation. Non-steroidal anti-inflammatory medications are commonly utilized to alleviate pain and address inflammation by blocking the production of PGE2 cyclooxygenase (COX). However, selective inhibition COX can easily lead series risks for cardiovascular diseases. Hence, it is imperative discover safer more efficient targets reducing Research has demonstrated that mPGES-1 serves as final enzyme controls rate prostaglandin...
The aim of the current study was to investigate potential role microRNA-183-5p (miR-183-5p) in proliferation, invasion and metastasis pancreatic cancer, identify promising target genes oncogenic miR‑183‑5p. Western blotting quantitative polymerase chain reaction (qPCR) were used whether these microRNAs may be useful as biomarkers carcinoma (PaCa). Potential verified using miRDB, PicTar TargetSCAN, qPCR detect expression miR‑183 suppressor cytokine signaling 6 (SOCS‑6; a miR-183) PANC‑1 PaCa...
Tumor necrosis factor-α (TNF-α) has been suggested to be a putative tumor promoter gene, and autocrine of TNF-α expression found in colon cancer ovarian cancer. As the role human gallbladder not yet elucidated, present study examined cancer-derived cell lines. Based on data, mRNA protein differed significantly different between In addition, using siRNA targeting TNF-α, vector, pGPU-GFP-siTNF-α, was constructed then transfected into SGC-996 cells (gallbladder line) which express high levels...
Background 2,5-dimethylcelecoxib (DMC) is a targeted inhibitor of microsomal prostaglandin E synthase-1 (mPGES-1), key enzyme in the PGE2 synthesis pathway inflammatory mediators. Previous studies have confirmed that DMC can inhibit growth hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). However, it not known whether involved changes tumor immune microenvironment. Methods In this study, we explored effects on HBV-related HCC microenvironment, and deeply analyzed its unique...
Background and Aim: Receptor interacting protein(RIP)-1 is thought to have a significant role in inflammation signaling pathways; however, the of RIP-1 malignant tumors largely unknown. Methods: The present study examined functions underlying mechanisms gallbladder cancer vitro vivo. In this we determined expression 60 clinical specimens from patients with 3 cell lines. Using siRNA targeting RIP-1, plasmid vectors (phU6-EGFP-puro/siRIP-1) were constructed transfected into cells characterize...
Abstract Carbamoyl phosphate synthase 1 (CPS1) is the rate‐limiting enzyme in first step of urea cycle and an indispensable metabolism human liver. However, CPS epigenetic regulation involves promoter analysis role liver‐enriched transcription factors ( LETF s), which not fully elucidated. In this work, region hCPS gene was cloned, its activity investigated. An , hepatocyte nuclear factor 3‐beta HNF 3β), found to promote transcriptional expression liver‐derived cell lines. addition,...
Previous studies have shown that transfection of the snake venom cystatin (sv-cystatin) gene can inhibit invasion and metastasis tumor cells. The aim this study was to investigate pharmaceutical applications sv-cystatin in melanoma therapy. We constructed a recombinant adenovirus carrying (Ad/sv-cystatin) control virus (Ad/null). Matrigel assays were used assess cell migration invasiveness vitro. antimelanoma effects Ad/sv-cystatin assessed syngeneic mouse model with an experimental lung...
Abstract Background The predominant immune cells in solid tumors are M2-like tumor-associated macrophages (M2-like TAMs), which significantly impact the promotion of epithelial-mesenchymal transition (EMT) tumors, enhancing stemness and facilitating tumor invasion metastasis. However, contribution TAMs to progression gallbladder cancer (GBC) is partially known. Methods Immunohistochemistry was used evaluate expression stem cell (CSC) markers 24 pairs GBC adjacent noncancerous tissues from...
We investigated the mechanism of caffeine in influencing HBx(+) hepatocytes to synthesize PGE2. The inhibitory effect on hepatocyte proliferation increased with increasing concentrations (200-800 μM) and treatment times (1-7 days), which was first observed at second test time point (caffeine for 4 days). inhibition growth HL7702-HBx HepG2-HBx cells most obvious 800 μM 7 days. PGE2 secretion expression mPGES-1 EGR1 were downregulated, whereas PPARγ upregulated. promoter activity decreased...
Helicobacter pylori is a well-known causative organism of chronic gastric diseases and has been found in many hepatic carcinoma samples. To explore the expression apoptosis-related proteins development H. pylori-infected livers, we utilized BALB/cAnSlac mice to establish an model by oral inoculation orthotopic grafts tumors H22 cells, respectively. We that colonies could not be cultured from all liver tumor However, its 16S rRNA was detectable 85.3% livers 66.7% infected mice. Inflammatory...
Background: HepG2/(ArgI+OTC)4 (previously constructed) is a recombinant human liver cell line with strong ability to reduce ammonia in vitro. However, its application value ex vivo has not been investigated. Objectives: To examine the efficacy of cells micro-bioartificial (micro-BAL) device for vivo. Methods: A simple micro-BAL containing microbioreactor and small-type peristaltic pump was installed. The rats hepatic failure were randomly divided into three groups (n = 10) treated different...