RNA interference-based gene therapy has led to a strategy for spinal cord injury (SCI) therapy. However, there have been high requirements regarding the optimal delivery vector siRNA-based SCI Here, we developed an injectable and photocurable lipid nanoparticle GelMA (PLNG) hydrogel scaffold controlled dual siRNA at wound site. The prepared PLNG could efficiently protect retain bioactivity of nanocomplex. It facilitated sustainable release along with degradation in 7 days. After loading...

Gene therapy has emerged as a new strategy for cancer therapy. As an alternative nucleic acid material, messenger ribonucleic (mRNA) is being increasingly utilized in gene However, unfulfilled requirements and lack of ideal mRNA delivery vectors persist.We developed advanced system, DMP-039, by fusing cell-penetrating peptide, cRGD-R9, cationic nano-sized DMP backbone together. The vector was synthesized the self-assembly DOTAP lipid mPEG-PCL polymer. Introduction cRGD-R9 peptide onto...

The study addresses the critical issue of accurately predicting ammonia nitrogen (NH3-N) concentration in a sequencing batch reactor (SBR) system, achieving reduced consumption through automatic control technology. NH3-N serves as key indicator treatment efficiency and environmental impact; however, its complex dynamics scarcity measurements pose significant challenges for accurate prediction. To tackle this problem, an innovative Transformer-long short-term memory (Transformer-LSTM) network...

Oral squamous cell carcinoma (OSCC) accounts for nearly 90% of oral and oropharyngeal cancer cases is characterized by high mortality poor prognosis. RNA-based gene therapies have been developed as an emerging option treatment, but it has not widely explored in OSCC. In this work, we efficient siRNA cationic micelle DOTAP-mPEG–PCL (DMP) self-assembling the lipid DOTAP monomethoxy poly(ethylene glycol)-poly(ε-caprolactone) (mPEG–PCL) polymer. We tested characteristics transformation...

Abstract mRNA‐based gene therapy has emerged as an advanced strategy for hepatocellular carcinoma (HCC) treatment. However, one of its main limitations is lacking delivery precision in vivo. Distinguishing HCC cells from other tissues crucial maintaining the high stability, positive therapeutic outcomes, and safety mRNA therapeutics. Here, a novel specific peptide HCC167 developed by phage display. It shown that can specifically recognize variety substrates including patient samples with...

Immunogene therapy provides a new strategy for the treatment of colorectal cancer. Compared to plasmid DNA, mRNA possesses several advantages as therapeutic nucleic acid material and shows high potential in cancer therapy. Although efforts have been made conquer limited efficiency delivery, most current vectors possess complex structures or compositions, which introduces additional toxicity hinders their further clinical application. Hence, it is highly necessary develop potent delivery...

Rheumatoid arthritis (RA) is one of the most common autoimmune diseases worldwide, causing severe cartilage damage and disability. Despite recent progress made in RA treatment, limitations remain achieving early efficient therapeutic intervention. Advanced strategies are high demand, siRNA-based technology with a gene-silencing ability represents new approach for treatment. In this study, we created cationic delivery micelle consisting low-molecular-weight (LMW) polyethylenimine...

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, with more than 300,000 new cases annually. Despite advances in existing treatments, including surgery, radiation, chemotherapy, and immunotherapy, overall survival prognosis have remained poor. However, gene therapy based on non-viral vectors provides ideas for treatment OSCC. Here, we aimed to prepare describe synthesis, biosafety, preclinical efficacy DOTAP-mPEG-PCL (DMP) OSCC therapy.We prepared a nano-sized...

This paper presents a wireless sensor based on spoof surface plasmon polaritons (SSPPs), which can be used to measure liquid level in non-metallic pipe real time. is flexible and bent wound around the at will. A pair of ultra-wideband antennas with orthogonal polarization directions were provided both ends receive excitation signals transmit signals. horn connected two ports VNA stimulate whole system. When pipeline changes, S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML"...

Abstract Purpose There are limited and no standard therapies for recurrent glioblastoma. We herein report the antitumour activity safety of sintilimab, bevacizumab temozolomide (TMZ) in Methods retrospectively analysed eight patients with glioblastoma treated sintilimab (200 mg) every three weeks + (10 mg/kg) TMZ mg/m²orally) (5 days orally 28 a total four weeks). The primary objective was investigator-assessed median progression-free survival(mPFS). Secondary objectives were to assess...

Cold stress can limit the growth and development of grapevines, which ultimately reduce productivity. However, mechanisms by grapevines respond to cold are not yet fully understood. Here, we characterized an APETALA2/ethylene response factor (AP2/ERF) was shown be a target gene our previously identified VaMYB4a from Amur grape. We further investigated molecular interactions between VaERF054-like transcription factors in grapes their role tolerance. Our results demonstrated that directly...

Melatonin (MEL) is an antioxidant molecule that enhances plant tolerance to environmental stress. However, the mechanisms by which MEL regulates cold signaling pathways in grapes under stress remain elusive. Here, we investigated physiological and transcriptomic changes grape seedlings treated with exogenous determine their protective role Results showed 150 μM effectively attenuated cold-induced cell damage reducing reactive oxygen species (ROS) preserving chloroplast structure function....

This study investigated the effect of exenatide on cardiac expression adiponectin receptor 1 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits heart function in streptozotocin-induced diabetic rats.Male Sprague-Dawley rats were randomly divided into four groups, i.e. control group, treated with low doses (2 μg · kg-1.d-1) high (10 kg-1.d-1). Diabetes was induced by intraperitoneal injection streptozotocin (65 mg/kg body weight). At termination after treatment for eight...

In this article, a sensor for real-time online monitoring of the rotor axial displacement an aeroengine based on microwave coaxial resonator (MCR) is proposed. During process, different displacements engine will make MCR introduce additional grounding capacitance, resulting in generating resonance statuses. Therefore, and warning can be achieved by reading characteristics real time. Furthermore, proposed also endowed with low-flow resistance since introducing design diversion hole. Finally,...

Introduction: Cell-membrane nanocarriers are usually constructed by modifying the nanoparticle surface with cell membrane extracts, which has a direct benefit in endowing targeting capacity to based on their original types. However, delivering nucleic acid cargos membrane–based nanoparticles is difficult owing strong negative charge of fraction. In this study, we developed cancer drug delivery system, cMDS, for efficient siRNA delivery. Meanwhile, cancer-specific immune response stimulated...