A5101837289- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Neurotoxicity Research
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Gut microbiota and health
- Chronic Lymphocytic Leukemia Research
- Nerve injury and regeneration
- Radiomics and Machine Learning in Medical Imaging
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Mass Spectrometry Techniques and Applications
- Anorectal Disease Treatments and Outcomes
- Chronic Myeloid Leukemia Treatments
- Enhanced Recovery After Surgery
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
- Functional Brain Connectivity Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Infant Nutrition and Health
- EEG and Brain-Computer Interfaces
- Neuroscience and Neural Engineering
- Virus-based gene therapy research
- Nuclear Physics and Applications
- Analytical Chemistry and Chromatography
- Lung Cancer Diagnosis and Treatment
Beijing Technology and Business University
2024
Fred Hutch Cancer Center
2022-2024
Pennsylvania State University
2015-2022
University of Washington
2022
Adult mammalian brains have largely lost neuroregeneration capability except for a few niches. Previous studies converted glial cells into neurons, but the total number of neurons generated is limited and therapeutic potential unclear. Here, we demonstrate that NeuroD1-mediated in situ astrocyte-to-neuron conversion can regenerate large functional new after ischemic injury. Specifically, using NeuroD1 adeno-associated virus (AAV)-based gene therapy, were able to one third caused by injury...
We have previously developed a cocktail of nine small molecules to convert human fetal astrocytes into neurons, but nine-molecule recipe is difficult for clinical applications. Here, we identify chemical formula with only three four astrocyte-to-neuron conversion. demonstrate that modulation signaling pathways among Notch, glycogen synthase kinase 3, transforming growth factor β, and bone morphogenetic protein sufficient change an astrocyte neuron. The chemically converted neurons can...
Abstract Ectopic expression of a single neural transcription factor NeuroD1 can reprogram reactive glial cells into functional neurons both in vitro and vivo, but the underlying mechanisms are not well understood yet. Here, we used RNA‐sequencing technology to capture transcriptomic changes at different time points during reprogramming process. We found that following overexpression, astroglial genes (ACTG1, ALDH1A3, EMP1, CLDN6, SOX21) were significantly downregulated, whereas neuronal...
Chemical reprogramming of astrocytes into neurons represents a promising approach to regenerate new for brain repair, but the underlying mechanisms driving this trans-differentiation process are not well understood. We have recently identified four small molecules - CHIR99021, DAPT, LDN193189, and SB431542 that can efficiently reprogram cultured human fetal functional neurons. Here we employ next generation RNA-sequencing technology investigate transcriptome changes during...
Shared bacteria between maternal breast milk and infant stool, infers that transfer of microbiota through breastfeeding seeds the establishment gut microbiome. Whether combination antiretroviral therapy (cART) impacts in women living with HIV is unknown. Since current standard care for people includes cART, it has been difficult to evaluate impact cART on Here, we performed a next-generation sequencing retrospective study from pre-ART era clinical trials Nairobi, Kenya (between 2003-2006...
ABSTRACT Mammalian brains have largely lost internal neural regeneration capability except for a few discrete neurogenic niches. After brain injury, the cerebral cortex is especially difficult to repair due its extremely low rate of adult neurogenesis. Previous studies converted glial cells into neurons, but total number neurons generated rather limited, casting doubt about therapeutic potential. Here, we demonstrate that high-efficiency neuroregeneration can be achieved in mammalian by...
The clinical management of patients with indeterminate pulmonary nodules is associated unintended harm to and better methods are required more precisely quantify lung cancer risk in this group. Here, we combine multiple noninvasive approaches accurately identify nodules. We analyzed 94 quantitative radiomic imaging features 41 qualitative semantic variables molecular biomarkers from blood derived an antibody-based microarray platform that determines protein, cancer-specific glycan,...
Abstract Purpose: Fusobacterium nucleatum (Fn) has been associated with colorectal cancer (CRC) etiology and outcomes. However, these relationships may differ by tumor attributes. We examined whether Fn enrichment presence in CRC was attributes survival. Experimental Design: measured the abundance of via droplet digital PCR patient-matched normal tissue samples from a subset participants Puget Sound Cohort (n=526). defined as continuous difference (normalized to counts host housekeeping...