A5044858738- Retinal Diseases and Treatments
- Complement system in diseases
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Retinal Imaging and Analysis
- Mosquito-borne diseases and control
- Microtubule and mitosis dynamics
- Blood Coagulation and Thrombosis Mechanisms
- Enzyme Structure and Function
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
- Mitochondrial Function and Pathology
- Retinal and Macular Surgery
- Blood groups and transfusion
- Adipokines, Inflammation, and Metabolic Diseases
- Kawasaki Disease and Coronary Complications
- Biochemical and Molecular Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Microbial Metabolic Engineering and Bioproduction
- Renal Diseases and Glomerulopathies
- Systemic Lupus Erythematosus Research
- Ocular Diseases and Behçet’s Syndrome
Medical University of South Carolina
2008-2021
Center for Neurosciences
2009
University of California, Santa Barbara
1980-1982
purpose. Polymorphisms in factor H (fH), an inhibitor of the alternative pathway (AP) complement activation, are associated with increased risk for age-related macular degeneration (AMD). The authors investigated therapeutic use a novel recombinant form fH, CR2-fH, which is targeted to sites mouse choroidal neovascularization (CNV). CR2-fH consists N terminus contains AP-inhibitory domain, linked receptor 2 (CR2) targeting fragment that binds activation products. methods. Laser-induced CNV...
During complement activation the C3 protein is cleaved, and fragments are covalently fixed to tissues. Tissue-bound a durable biomarker of tissue inflammation, these have been exploited as addressable binding ligands for targeted therapeutics diagnostic agents. We generated cross-reactive murine monoclonal antibodies against human mouse C3d, final degradation fragment during activation. developed 3 (3d8b, 3d9a, 3d29) that preferentially bind iC3b, C3dg, C3d in solution, but do not intact or...
Neovascular age-related macular degeneration (AMD) is characterized by choroidal neovascularization (CNV). An overactive complement system associated with AMD pathogenesis, and serum pro-inflammatory cytokines, including IL-17, are elevated in patients. IL-17 produced C5a-receptor-expressing T-cells. In murine CNV, infiltrating γδT- rather than Th17-cells produce the measurable lesioned eyes. Here we asked whether C5a generated locally response to CNV recruits IL-17-producing T-cells eye....
Background Age-related macular degeneration (AMD), a complex disease involving genetic variants and environmental insults, is among the leading causes of blindness in Western populations. Genetic histologic evidence implicate complement system AMD pathogenesis; smoking major risk factor associated with increased risk. Although previous studies have demonstrated that cigarette smoke exposure (CE) retinal pigment epithelium (RPE) defects mice, leads to activation patients, it unknown whether...
Autophagy is a lysosomal machinery-dependent process that catabolizes cellular components/organelles and proteins in an autophagic vacuole (AV)-dependent -independent manner, respectively. Short-term exposure of the retina to bright light results shortening outer segments, concomitant with AV formation. also induced by continuous long-term damage, leading photoreceptor cell death. Here authors examined two questions: autophagy during damage proapoptotic or antiapoptotic, are rods cones...
Purpose.: Complement factor B (CFB) is a required component of the alternative pathway (AP) complement, and CFB polymorphisms are associated with age-related macular degeneration (AMD) risk. made in liver, but expression has also been detected retina retinal pigment epithelium (RPE)-choroid. We investigated whether production by RPE can promote AP activation mouse choroidal neovascularization (CNV). Methods.: Transgenic mice expressing under RPE65 promoter were generated crossed onto...
Purpose: Genetic associations and the presence of complement components within pathological structures age-related macular degeneration (AMD) have generated hypothesis that AMD is caused by chronic local activation. Since majority activity in common terminal pathway results from engagement amplification loop, alternative has been proposed as a logical therapeutic target. We recently factor H (fH)-based inhibitor (CR2-fH) with capacity to be "targeted" sites C3 asked whether human (TT30)...
&Very low density lipoproteins (6-VLDL) isolated from cholesterol-fed rhesus monkeys stimulated cholesteryl ester synthesis and accumulation in mouse peritoneal macrophages.The apoprotein specificity requirement for the cell surface uptake of j3-VLDL was investigated by treating with trypsin (8-VLDL (T)), incubating @-VLDL (T) other or apoproteins, reisolating 8-VLDL measuring its biological activity which, this study, is defined as ability lipoprotein to stimulate cholesterol esterification...
purpose. RPE65, a major retinal pigment epithelium protein, is essential in generating 11-cis retinal, the chromophore for all opsins. Without chromophore, cone opsins are mislocalized and cones degenerate rapidly (e.g., Rpe65 −/− mouse). Function, survival, correct targeting of increased on supplying retinal. Here, we determine consequences withdrawal supplementation development all-cone Nrl retina. methods. −/−, wild-type mice were examined. Cone structure was analyzed by using TUNEL...
Abstract Laser-induced photocoagulation of RPE-Bruch's membrane is a conventional method inducing CNV in mice, which resembles many aspects the pathology human exudative age-related macular degeneration (AMD). The presence complement components and inflammatory cells AMD lesions support an "inflammation-related theory AMD". Here we further examined processes experimentally-induced mice. spleens mice were enlarged on day 6, with corresponding increase weight cellularity spleenic mass. In...