A5012993340- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Mast cells and histamine
- Blood Coagulation and Thrombosis Mechanisms
- Vitamin K Research Studies
- Enzyme function and inhibition
- Hemophilia Treatment and Research
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Receptor Mechanisms and Signaling
- Complement system in diseases
- Polyamine Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- Click Chemistry and Applications
- Protease and Inhibitor Mechanisms
- Phytochemical compounds biological activities
- Lipid Membrane Structure and Behavior
- PI3K/AKT/mTOR signaling in cancer
- Amino Acid Enzymes and Metabolism
- RNA Interference and Gene Delivery
- Nuclear Receptors and Signaling
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
University of Cincinnati
2024
Charité - Universitätsmedizin Berlin
2024
Harvard University
2024
Hospital Universitario Fundación Jiménez Díaz
2024
Cochin University of Science and Technology
2023
Medical University of South Carolina
2011-2022
BioCryst Pharmaceuticals (United States)
2019-2022
Vaidyaratnam P.S. Varier Ayurveda College
2019-2021
Pulmonary and Allergy Associates
2005
College of Charleston
2004
High molecular weight kininogen (HK) and factor XII are known to bind human umbilical vein endothelial cells (HUVEC) in a zinc-dependent saturable manner indicating that HUVEC express specific binding site(s) for those proteins. However, identification immunochemical characterization of the putative receptor has not been previously accomplished. In this report, we have identified cell surface glycoprotein is likely candidate HK site on HUVECs. When solubilized membranes were subjected an...
Bradykinin is a major mediator of swelling in C1 inhibitor deficiency as well the angioedema seen with ACE inhibitors and may contribute to bronchial hyperreactivity asthma. Formation bradykinin occurs fluid phase along cell surfaces requiring interaction factor XII, prekallikrein, high M(r) kininogen (HK). Recent data suggest that activation kinin-forming cascade can occur on surface endothelial cells, even absence XII. We sought further define this XII-independent mechanism kinin...
Neovascular age-related macular degeneration (AMD) is characterized by choroidal neovascularization (CNV). An overactive complement system associated with AMD pathogenesis, and serum pro-inflammatory cytokines, including IL-17, are elevated in patients. IL-17 produced C5a-receptor-expressing T-cells. In murine CNV, infiltrating γδT- rather than Th17-cells produce the measurable lesioned eyes. Here we asked whether C5a generated locally response to CNV recruits IL-17-producing T-cells eye....
Summary The physiologic activation of the plasma kallikrein-kinin system requires assembly its constituents on a cell membrane. High-molecular-weight kininogen (HK) and cleaved HK (HKa) both interact with at least three endothelial binding proteins: urokinase plasminogen activator receptor (uPAR), globular C1q (gC1qR,) cytokeratin 1 (CK1). affinity HKa for cells are KD=7–52 nM. contribution each protein is unknown. We examined direct to soluble extracellular form uPAR (suPAR), gC1qR CK1...
Purpose.: Complement factor B (CFB) is a required component of the alternative pathway (AP) complement, and CFB polymorphisms are associated with age-related macular degeneration (AMD) risk. made in liver, but expression has also been detected retina retinal pigment epithelium (RPE)-choroid. We investigated whether production by RPE can promote AP activation mouse choroidal neovascularization (CNV). Methods.: Transgenic mice expressing under RPE65 promoter were generated crossed onto...
Summary Cell surface proteins reported to participate in the binding and activation of plasma kinin-forming cascade includes gC1qR, cytokeratin 1 u-PAR. Each these binds high molecular weight kininogen (HK) as well Factor XII. The studies on interaction proteins, using dot-blot analysis, revealed that both gC1qR u-PAR while do not bind each other. properties were further analyzed by gel filtration. When biotinylated was incubated with either or filtered, a new, higher peak containing biotin...
Cellular expression of the beta(2)-adrenergic receptor (beta(2)-AR) is suppressed at translational level by 3'-untranslated region (UTR) sequences. To test possible role 3'-UTR-binding proteins in suppression beta(2)-AR mRNA, we expressed full-length 3'-UTR or adenylate/uridylate-rich (A+U-rich element (ARE)) RNA from sequences cell lines that endogenously express this receptor. Reversal repression retroviral suggested ARE RNA-binding are involved expression. Using a 20-nucleotide as an...