A5042402836- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Testicular diseases and treatments
- Lung Cancer Research Studies
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Lung Cancer Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Sarcoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Occupational and environmental lung diseases
- Oral and Maxillofacial Pathology
- Metastasis and carcinoma case studies
- Cancer Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Neuroblastoma Research and Treatments
- Sinusitis and nasal conditions
- Pleural and Pulmonary Diseases
- T-cell and Retrovirus Studies
- Tuberculosis Research and Epidemiology
- Ethics in Clinical Research
Hospital de Câncer de Barretos
2015-2025
Hospital São Paulo
2023
University of Rochester
2009
Background For non-small cell lung cancer (NSCLC) the most used method for analysing programmed death ligand 1 (PD-L1) expression is Tumor Proportion Score (TPS). Nevertheless, other tumour types, Combined Positive (CPS) has been of choice. Aim Evaluate and compare predictive value both CPS TPS as predictors immunotherapy response in NSCLC, to evaluate agreement intra-observer between methods inter-observer two expert pathologists. Methods 56 NSCLC patients who were treated with...
Testicular germ cell tumors (TGCT) represent the second main cause of cancer-related death in young men. Despite high cure rates, refractory disease results poor prognosis. Epigenetic reprogramming occurs during development seminomas and non-seminomas. Understanding molecular genetic basis these would an important advance search for new TGCT markers. Hence frequency methylation a gene panel (VGF, MGMT, ADAMTS1, CALCA, HOXA9, CDKN2B, CDO1 NANOG) was evaluated 72 primary by quantitative...
Background: Thyroid nodules can be identified in up to 68% of the population. Fine-needle aspiration (FNA) cytopathology classifies 20%-30% as indeterminate, and these are often referred for surgery due risk malignancy. However, histological postsurgical reports indicate that 84% cases benign, highlighting a high rate unnecessary surgeries. We sought develop validate microRNA (miRNA)-based thyroid molecular classifier precision endocrinology (mir-THYpe) with both sensitivity specificity,...
Abstract Lung cancer is the deadliest worldwide. The mutational frequency of EGFR and KRAS genes in lung adenocarcinoma varies worldwide per ethnicity smoking. impact mutations Brazilian remains poorly explored. Thus, we investigated a large series together with patients’ genetic ancestry, clinicopathological sociodemographic characteristics. was 22.7% 20.4%. average ancestry proportions were 73.1% for EUR, 13.1% AFR, 6.5% AME 7.3% ASN. independently associated never-smokers, high-Asian...
Testicular Germ Cell Tumors (TGCTs) are a rare group of neoplasms and the most common solid malignancy arising in young male adults. Despite good response these tumors to platinum-based chemotherapy, some patients refractory treatment present poor clinical outcomes. During carcinogenesis tumor development, cancer cells reprogram energy metabolism towards hyper-glycolytic phenotype, an emerging hallmark cancer. This phenomenon, known as Warburg effect or aerobic glycolysis, involves...
TP53 is the most frequently mutated gene in lung tumors, but its prognostic role admixed populations, such as Brazilians, remains unclear. In this study, we aimed to evaluate frequency and clinicopathological impact of mutations non-small cell cancer (NSCLC) patients Brazil.We analyzed 446 NSCLC from Barretos Cancer Hospital. mutational status was evaluated through targeted next-generation sequencing (NGS) variants were biologically classified disruptive/nondisruptive...
Homeobox A9 promoter methylation (HOXA9) has been reported as a biomarker for early lung adenocarcinoma patients' prognosis. We aim to evaluate its prognostic value, regardless of disease stage. Using droplet digital PCR, we measured HOXA9 in cohort comprising 161 Brazilian patients. Low was associated with higher cancer-specific survival but showed no significance after adjustment clinical covariates. While low earlier stages, association observed this subset Overall, is not an independent...
Immune checkpoint inhibitors (ICIs) revolutionized non-small-cell lung cancer (NSCLC) treatment. However, improving patients' selection for this therapy is needed. Gene expression profile (GEP) a promising biomarker tool. We assessed the predictive value of 48 onco-immune GEPs in an NSCLC real-world scenario.Retrospective cohort Brazilian patients treated with ICIs any line. GEP was FFPE tumor tissue using nCounter PanCancer IO360 panel, comprising 770 immune genes.The median age 135 61...
Testicular germ cell tumors (TGCT) are the most common malignant neoplasm in young men. DNA mismatch repair deficiency can lead to microsatellite instability (MSI), an important mechanism of genetic instability. A mutation BRAF gene has been implicated pathogenesis several solid and recently become therapeutic target. The role MSI TGCT, particularly refractory disease, is poorly understood reported findings controversial. In this study, we aimed determine frequency clinical impact status...
Objective: To review the pathological diagnosis of possible cases and/or hidden malignant mesothelioma (MM) between 2000 and 2012 using Hospital-Based Cancer Registry database in state São Paulo, Brazil. Methods: Possible were retrieved by assessing database. Inclusion criteria being older than 30 years age having ICD-O-3 topography morphology codes related to MM. A board expert pathologists reviewed pathology reports requested paraffin blocks that demanded revision. After staining with...
Abstract Introduction Lung cancer in never-smoker (LCINS) patients accounts for 20% of lung cases, and its biology remains poorly understood, particularly genetically admixed populations. We elucidated the molecular profile driver genes Brazilian LCINS. Methods The mutational gene fusion status 119 adenocarcinomas from self-reported patients, was assessed using targeted sequencing (NGS), nCounter, immunohistochemistry. A panel 46 ancestry-informative markers determined patients’ genetic...
Background: Malignant pleural mesothelioma (MPM) is a highly lethal disease comprising heterogeneous group of tumors with challenging to predict biological behavior. The diagnosis complex, and the histologic classification includes 2 major subtypes MPM: epithelioid (∼60% cases) sarcomatous (∼20%). Its identification depends upon pathological investigation supported by clinical radiological evidence more recently ancillary molecular testing. Treatment options are currently limited, no known...
The abnormal activation of telomerase, codified by the telomerase reverse transcriptase (TERT) gene, is related to one cancer hallmarks. Hotspot somatic mutations in promoter region TERT, specifically c.-124:C>T and c.-146:C>T, were recently identified a range human cancers have been associated with more aggressive behavior. Testicular germ cell tumors frequently exhibit good prognosis; however, development refractory disease still clinical challenge. In this study, we aim evaluate for first...
Microsatellite instability (MSI) in non-small cell lung cancer (NSCLC) is uncommon; however, most studies refer to European and Asian populations. There are currently no data on MSI frequency highly admixed populations, such as the one represented by Brazilian NSCLC patients.This study aimed evaluate of patients.We evaluated 526 patients diagnosed with at Barretos Cancer Hospital (Brazil). The molecular evaluation was performed using a hexa-plex marker panel polymerase chain reaction...
Summary The T‐box transcription factor Brachyury has been considered a cancer‐specific marker and novel oncotarget in solid tumors. overexpression described various cancers, being associated with epithelial–mesenchymal transition, metastasis, poor prognosis. However, its clinical association testicular germ cell tumor is unknown. We analyzed the expression of by immunohistochemistry series well‐characterized samples at transcript level silico analysis. Additionally, we aimed to investigate...
Metabolic reprogramming is one of the hallmarks cancer. The hyperglycolytic phenotype often associated with overexpression metabolism-associated proteins, such as monocarboxylate transporters (MCTs). MCTs are little explored in germ cell tumors (GCTs), thus, opportunity to understand relevance these metabolic markers and their chaperone CD147 this type tumor arises. main aim study was evaluate expression MCT1, MCT2, MCT4 testicular GCT samples clinicopathological significance metabolism...
Recently, the agents AMG-510 (sotorasib, Amgen, Thousand Oaks, CA) and MRTX849 (adagrasib, Mirati Therapeutics, San Diego, were developed to target KRAS p.Gly12Cys mutation. 24,255][26] In a phase I study, 32.2% (19 out of 59) sotorasib-treated patients presented with objective response, 88.1% (52 disease control. 26In II 94% (17 18) adagrasib-treated lung control, response was not yet available (KRYSTAL-1 study; ClinicalTrials.govidentifier: NCT03785249). 25e frequency in admixture NSCLC...
microRNAs (miRNAs) are small endogenous noncoding RNAs, and alterations in their expression may contribute to oncogenesis. Discovering a unique miRNA pattern holds the potential for early detection novel treatment possibilities cancer. This study aimed evaluate pediatric patients with gonadal germ cell tumors (GCTs), focusing on characterizing profiles of each histological subtype identifying distinct signature total 42 samples GCTs. The analysis revealed all types, regardless primary site....
Intratumoral similarities and differences between large-cell neuroendocrine carcinomas (LCNECs) small-cell lung (SCLCs) are determined partially by the Notch signaling pathway, which controls switch from to slight/non-neuroendocrine cell fate. LCNECs divided into two subgroups according genomic alterations: type I exhibit a profile characterized achaete‐scute homolog 1 (ASCL1)high/delta-like protein 3 (DLL3)high/NOTCHlow II show pattern ASCL1low/DLL3low/NOTCHhigh. Here, we used...
Abstract Background Targeted and immunotherapies are currently moving toward early-stage settings for patients with non-small cell lung cancer (NSCLC). Predictive biomarkers data scarce in this scenario. We aimed to describe the frequency of EGFR mutations PD-L1 expression levels non-squamous NSCLC from a large, single Brazilian oncology center. Methods retrospectively evaluated diagnosed at an (IB IIIA-AJCC seventh edition) Barretos Cancer Hospital (n = 302). mutational status was assessed...
Abstract ERBB2 exon 20 insertions may impact the clinical management of lung cancer patients. However, frequency in patients Brazil is scarce. Here, we analyzed 722 Brazilian non‐small cell (NSCLC) from Barretos Cancer Hospital that were indicated to require routine molecular testing. evaluated by a targeted panel using next‐generation sequencing (NGS). Clinicopathological and data collected patient medical records. Among NSCLC patients, 85.2% had adenocarcinomas, 53.9% male, 66.8% quitter...
Gene fusions have been successfully employed as therapeutic targets for lung adenocarcinoma. However, tissue availability molecular testing of multiples alterations is frequently unfeasible. We aimed to detect the presence ALK, RET, and ROS1 rearrangements by a RNA-based single assay in Brazilian adenocarcinomas associate with clinicopathological features genetic ancestry.From FFPE series 444 molecularly characterized adenocarcinomas, 253 EGFR/KRAS wild-type cases were eligible gene...
Cancer of unknown primary origin (CUP) is defined as metastatic cancer without identification the site. Considering that only 15-20% patients with CUP show a favorable outcome, identifying biomarkers may help improve clinical management who do not respond well to conventional therapies. In this context, study metabolic profile pave way establish new and/or therapeutic targets; therefore, aimed characterize expression metabolism-related proteins in CUP. The monocarboxylate transporters MCT1,...
Dentinoid has been mentioned as a frequent component in several types of benign odontogenic tumors; however, there are some other very rare dentinoid-producing tumors that have described, which not recognized the current World Health Organization Histological Classification Odontogenic Tumours. In this context, we report an unusual malignant tumor containing dentinoid located left maxilla 41-year-old man. The lesion was initially diagnosed and treated cemento-ossifying fibroma. After 7...